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pFastBac(His10-Lckg2a,Y394F)

Plasmid

#177893

Purpose

Baculo expression of His10 tag fused to human LckG2A

Depositor

Ron Vale

Article

Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

Insert

Lck (LCK Human)

Tags

10xHis-TEV

Expression

Insect

Mutation

G2A,Y394F

Promoter

Polyhedrin

Availability

Academic Institutions and Nonprofits only

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MS2-adRNA (5'GAC)

Plasmid

#170127

Purpose

Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-ADAR2(5'GAC)-MS2

Use

Lentiviral

Expression

Mammalian

Promoter

Human U6 and mouse U6

Availability

Academic Institutions and Nonprofits only

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MS2-adRNA (3'GAC)

Plasmid

#170129

Purpose

Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-ADAR2(3'GAC)-MS2

Use

Lentiviral

Expression

Mammalian

Promoter

Human U6 and mouse U6

Availability

Academic Institutions and Nonprofits only

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pIVT_goldDn29-P2A-GFP

Plasmid

#247163

Purpose

In vitro transcription of human codon optimized engineered goldDn29-P2A-GFP

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

goldDn29-P2A-GFP

Use

In vitro transcription

Tags

6xHis

Mutation

M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N

Promoter

mutated T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pIVT_Dn29-dCas9-P2A-GFP

Plasmid

#247165

Purpose

In vitro transcription of human codon optimized fusion of Dn29-dCas9-P2A-GFP

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

Dn29-dCas9-P2A-GFP

Use

In vitro transcription

Tags

SV40 NLS

Promoter

mutated T7

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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SpCas9-NG

Plasmid

#138566

Purpose

Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

SpCas9-NG

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…

Promoter

EFS

Available Since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pINDUCER21 (ORF-EG)

Plasmid

#46948

Purpose

Inducible lentiviral gene expression vector. Chloramphenicol cm + ccdB cassette may be removed to clone in gene of interest

Depositor

Stephen Elledge, Thomas Westbrook

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Available Since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only

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pINDUCER11 (miR-RUG)

Plasmid

#44363

Purpose

Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoR1; 1.4kb), can be replaced w mir30 based hairpin of interest

Depositor

Thomas Westbrook

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Insert

PheS Gly294

Use

Lentiviral

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pAV-H1promoter-2xTetO-High_ribosome_load-Tornado-2xALFA-10xSunTag-Xbp1(S255A)

Plasmid

#231595

Purpose

Express high ribosome loading circular RNA translation reporter 2xALFATag-10xSunTag-Xbp1(S255A) pause site under H1 promoter

Depositor

Marvin Tanenbaum

Article

Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

Insert

Kozak sequence-Tornado-2xALFATag-10xSunTag-Xbp1(S255A) pause sequence

Expression

Mammalian

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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phASCL1-N106

Plasmid

#31781

Depositor

Jerry Crabtree

Article

Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

Insert

achaete-scute complex homolog 1 (ASCL1 Human)

Use

Lentiviral

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pRSV-CaMKII(1-290)

Plasmid

#45065

Purpose

Expression of constit active CaMKII

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

CaM Kinase II (Camk2a Rat)

Expression

Mammalian

Mutation

truncated at amino acid 290

Promoter

RSV

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pLEX_305-C-dTAG

Plasmid

#91798

Purpose

Lentiviral/gateway cloning vector for C-terminally tagging proteins of interest with the dTAG system

Depositor

James Bradner, Behnam Nabet

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Type

Empty backbone

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Promoter

hPGK

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-Hsp-Fluc-PGK-Rluc-mCherry

Plasmid

#191863

Purpose

Heat inducible Fluc reporter with constitutive Rluc

Depositor

Yingxiao Wang

Article

Wu et al Nat Biomed Eng. 2021 Nov;5(11):1336-1347

Inserts

Firefly luciferase Fluc
Renilla luciferase Rluc

Use

Lentiviral

Tags

P2A-mCherry

Expression

Mammalian

Promoter

HSPA7 promoter and PGK promoter

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pInducer10-mir-RUP-PheS

Plasmid

#44011

Purpose

Lentiviral shRNA vector (pGIPz based) for inducible knockdown

Depositor

Stephen Elledge

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Insert

PheS Gly294

Use

Lentiviral

Tags

RFP

Expression

Mammalian

Promoter

TRE2

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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DHB-mVenus

Plasmid

#136461

Purpose

Fluorescently tagged segment of DNA Helicase B that translocates from the nucleus to the cytoplasm in response to phosphorylation by CDK2

Depositor

Tobias Meyer, Sabrina Spencer

Article

Spencer et al Cell. 2013 Oct 10;155(2):369-83. doi: 10

Insert

DNA Helicase B (HELB Human)

Use

Lentiviral

Tags

mVenus

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pEF-GFP

Plasmid

#11154

Purpose

Mammalian expression vector for expression of GFP (EF1a promoter)

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Has Service

Cloning Grade DNA

Insert

EF1 alpha promoter (EEF1A1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pET SUMO NL-FKBP-Cas9

Plasmid

#162730

Purpose

Bacterial expression, protein expression

Depositor

Amit Choudhary

Article

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Insert

FKBP F36V

Tags

6xHis and SUMO

Expression

Bacterial

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pET30a-GABAT

Plasmid

#239572

Purpose

Expresses E. coli GABA transaminase (GABA-T/gabT) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography.

Depositor

William Plaxton

Article

Benidickson et al Biol Methods Protoc. 2025 Jun 20;10(1):b

Insert

4-aminobutyrate aminotransferase (gabT E. coli)

Tags

His6

Expression

Bacterial

Available Since

July 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET30a-SSDH

Plasmid

#239573

Purpose

Expresses E. coli succinate semialdehyde dehydrogenase (SSDH/gabD) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography.

Depositor

William Plaxton

Article

Benidickson et al Biol Methods Protoc. 2025 Jun 20;10(1):b

Insert

Succinate-semialdehyde dehydrogenase (gabD E. coli)

Tags

His6

Expression

Bacterial

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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PB-TRE3G-MYCN

Plasmid

#104542

Purpose

PiggyBac vector encoding doxycycline-inducible human MYCN

Depositor

David Vereide

Article

Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

Insert

MYCN proto-oncogene, bHLH transcription factor (MYCN Human)

Expression

Bacterial and Mammalian

Promoter

TRE3G

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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