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pGL3-DICER-Prom

Plasmid

#25851

Depositor

David E. Fisher

Article

Levy et al Cell. 2010 Jun 11. 141(6):994-1005.

Insert

Dicer promoter (DICER1 gene ID 23405, Human)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 25, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-GPD1

Plasmid

#32487

Depositor

Pere Puigserver

Article

Kelly et al Mol Cell Biol. 2011 Jul;31(13):2696-706.

Insert

GPD1 (Gpd1 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only
pSUPER-retro-puro-shSTIM1

Plasmid

#89816

Purpose

Knockdown human STIM1 expression

Depositor

Shengyu Yang

Article

Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

Insert

STIM1 shRNA (STIM1 Human)

Use

Retroviral

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FAM20C:V5

Plasmid

#63584

Purpose

FAM20C can phosphorylate secreted phosphoproteins, and characterization of its activity identifies FAM20C as a Golgi casein kinase.

Depositor

Kenneth Irvine

Article

Ishikawa et al PLoS One. 2012;7(8):e42988. doi: 10.1371

Insert

FAM20C (Fam20c Mouse)

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only
pFB-Flag-MyoD-E12

Plasmid

#25990

Depositor

Jeffrey Dilworth

Article

Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

Insert

MyoD-E12 Heterodimer Fusion (Myod1 Mouse)

Use

Baculovirus transfer vector

Tags

Flag

Available Since

Sept. 8, 2010

Availability

Academic Institutions and Nonprofits only

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GST-PS-Grb2 1-154 (XSB382)

Plasmid

#78510

Purpose

Construct for expressing Grb2 lacking the C-terminal SH3 domain in E. Coli

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

Grb2 1-154 (GRB2 Human)

Tags

GST followed by a prescission protease claevage s…

Expression

Bacterial

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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CMV:eGFP-p2A-HA-GPR151(p.Arg95Ter)

Plasmid

#190134

Purpose

expresses eGFP-p2A-HA-GPR151 (pArg95Ter variant) in mammalian cells

Depositor

Joshua Knowles

Article

Bielczyk-Maczynska et al Nat Commun. 2022 Dec 1;13(1):7408. doi:

Insert

eGFP-p2A-HA-GPR151(p.Arg95Ter)

Use

Lentiviral

Tags

HA, eGFP, and p2A

Expression

Mammalian

Mutation

p.Arg95Ter mutation

Promoter

CMV

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shCcnb2

Plasmid

#162794

Purpose

Ccnb2 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Ccnb2 shRNA (Ccnb2 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_UBA/UIM-mut

Plasmid

#187152

Purpose

mammalian cell expression of FLAG-HUWE1 M1328A/F1330A/A1376G/S1382A

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

1xFLAG

Expression

Mammalian

Mutation

M1328A/F1330A/A1376G/S1382A

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBABE hygro MEN1 A242V

Plasmid

#11022

Depositor

Matthew Meyerson

Article

Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

Insert

MEN1 A242V (MEN1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

A242V

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
pGL3-Basic-hCYP26A1-FL- luciferase

Plasmid

#135566

Purpose

Full Length promoter of the human CYP26A1 gene in pGL3-Basic-luciferase

Depositor

Catharine Ross

Article

Zolfaghari et al J Cell Biochem. 2014 Oct;115(10):1740-51

Insert

Full-length promoter of human retinoic acid receptor-beta gene

Promoter

hHuman CYP26A1 gene promoter

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pFB-Flag-MyoD-MyoD

Plasmid

#25992

Depositor

Jeffrey Dilworth

Article

Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

Insert

MyoD-MyoD homodimer (Myod1 Mouse)

Use

Baculovirus transfer vector

Tags

Flag

Available Since

Sept. 8, 2010

Availability

Academic Institutions and Nonprofits only
MO70-N-Flag-hCRACR2A-isoform-a

Plasmid

#79592

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

Insert

CRACR2A-isoform-a (CRACR2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
GRB2-SHC

Plasmid

#139623

Purpose

StrepIII-HA-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

GRB2 (GRB2 Human)

Tags

StrepIII-HA

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCrysβ:ECFP-LexOP:mCherry-NRasQ61K

Plasmid

#89886

Purpose

Human NRasQ61K fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

Depositor

Tatjana Sauka-Spengler

Article

Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

Insert

mCherry-NRasQ61K

Use

Unspecified

Tags

mCherry

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag MFF iso2 WT

Plasmid

#74389

Purpose

Flag tagged human MFF isoform 2

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Tags

Flag

Expression

Mammalian

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
pShuttle-EGFP-CLASP2 340-1362

Plasmid

#24372

Depositor

Torsten Wittmann

Article

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

CLASP2 (340-1362) (CLASP2 Human)

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Mutation

CLASP2 deletion mutant that retains MT lattice bi…

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

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pCDNA5 TEX264wt-cSSH

Plasmid

#220209

Purpose

Mammalian expression of wild type TEX264, C-terminal twin-Strep_HA tag

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Tags

Strep-strep-HA

Expression

Mammalian

Promoter

CMV/TetO

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR_Mm_CEP55(WT)

Plasmid

#136333

Purpose

Mouse CEP55 cDNA in Gateway pENTR

Depositor

Guillermo de Cárcer, Marcos Malumbres

Article

de Carcer et al Nat Commun. 2018 Aug 1;9(1):3012. doi: 1

Insert

CEP55 (Cep55 Mouse)

Use

Gateway entry vector

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-E6AP Hect

Plasmid

#165102

Purpose

bacterial expression of a GST fusion of Human E6AP Hect domain

Depositor

Arthur Haas

Article

Ronchi et al Methods Mol Biol. 2012;832:197-218. doi:

Insert

E6AP Hect (UBE3A Human)

Tags

GST from plasmid.

Expression

Bacterial

Mutation

E6AP Hect domain Amino acids 495-738 (5'QLNP…

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Lem2

Plasmid

#120245

Purpose

Expresses V5-tagged Lem2 in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Lem2 (Lemd2 Mouse)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-HA-human CMYC (277-439)

Plasmid

#203435

Purpose

Mammalian expression of MYC

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

MYC (MYC Human)

Tags

2 x HA

Expression

Mammalian

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_ELAVL1

Plasmid

#106106

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting ELAVL1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

gRNA targeting ELAVL1 (ELAVL1 Human)

Use

CRISPR

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV neo KD Akt1

Plasmid

#14550

Depositor

Joan Brugge

Article

Irie et al J Cell Biol. 2005 Dec 19. 171(6):1023-34

Insert

Akt1 KD (Akt1 Mouse)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

Kinase Dead Mutant: K179M

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
Arrb1-Q394L

Plasmid

#35402

Depositor

Robert Lefkowitz

Article

Hara et al Nature. 2011 Aug 21;477(7364):349-53. do

Insert

beta-arrestin-1 (Arrb1 Rat)

Tags

Flag

Expression

Mammalian

Mutation

Q394L

Promoter

CMV

Available Since

March 2, 2012

Availability

Academic Institutions and Nonprofits only
AA145

Plasmid

#215937

Purpose

Fragmid fragment: (guide cassette) guide expression; Chen F+E trRNA (preferred)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v4 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pETM33_CLTC_Clathrin propeller

Plasmid

#178471

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

CLTC_Clathrin propeller (CLTC Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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MIGR1-CD5

Plasmid

#159329

Purpose

Over-expression of mouse CD5

Depositor

Nevil Singh

Article

Matson et al Proc Natl Acad Sci U S A. 2020 Jun 23;11

Insert

CD5 (Cd5 Mouse)

Use

Retroviral

Promoter

LTR

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pBactin-Halo-Tag

Plasmid

#196898

Purpose

Expression of Halo-Tag. Use as control for Halo-tag fusions

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Halo-Tag

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pET-45b-RUNX2(WT)-IDR-mCherry

Plasmid

#145286

Purpose

Bacterial Expression of 6xHis RUNX2(WT) IDR mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

RUNX2(WT)-IDR (RUNX2 Human)

Expression

Bacterial

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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