165,335 results

1. pGL3-sgRab18.N-Cas9-T2A-mCherry-P2A-Puro

   Plasmid

   #129418

   Purpose

   Encoding Cas9 and sgRAB18.N for CRISPR/Cas9 mediated HDR tagging of endogenous human RAB18 N-terminus

   Depositor

   Elina Ikonen

   Article

   Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

   Insert

   SpCas9 and sgRAB18.N (RAB18 Human)
   

   Use

   CRISPR

   Tags

   T2A-mCherry-P2A-Puro

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-SENP3

   Plasmid

   #34554

   Depositor

   Mary Dasso

   Article

   Yun et al J Cell Biol. 2008 Nov 17;183(4):589-95.

   Insert

   SENP3 (SENP3 Human)
   

   Tags

   EGFP

   Promoter

   CMV

   Available Since

   Dec. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYZ463

   Plasmid

   #187971

   Purpose

   TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

   Depositor

   Jef Boeke

   Article

   Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

   Insert

   Cas9 (codon optimized for human)
   

   Use

   Synthetic Biology

   Tags

   SV40

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-GGamma9-GFP2

   Plasmid

   #140991

   Purpose

   Encodes a G gamma subunit (GNG9) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma9-GFP2 (GNGT2 Human)
   

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ubi:Zebrabow

   Plasmid

   #118219

   Purpose

   For ubiquitous expression of Brainbow in zebrafish

   Depositor

   Alex Schier, Albert Pan

   Article

   Pan et al Development. 2013 Jul;140(13):2835-46. d

   Insert

   Brainbow 1.0 L
   

   Use

   Zebrafish expression

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCV-Blasticidin

   Plasmid

   #75085

   Purpose

   retrovirus-mediated gene transfer into mammalian cells

   Depositor

   David Mu

   Article

   Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. HA WDR24 pRK5

   Plasmid

   #46335

   Depositor

   David Sabatini

   Article

   Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

   Insert

   WDR24 (WDR24 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV BiP-Myc-KDEL-wt

   Plasmid

   #27164

   Purpose

   Mammalian expression of human Bip with Myc tag near the C-terminus, retaining the C-terminal KDEL ER localization sequence

   Depositor

   Ron Prywes

   Article

   Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

   Insert

   BiP (HSPA5 Human)
   

   Tags

   KDEL and Myc

   Expression

   Mammalian

   Available Since

   Jan. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCdrA-gfpC

   Plasmid

   #111614

   Purpose

   Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

   Depositor

   Tim Tolker-Nielsen

   Article

   Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

   Insert

   PcdrA-RBSII-gfp(Mut3)-T0-T1
   

   Expression

   Bacterial

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pInducer-YAP1

    Plasmid

    #213583

    Purpose

    Doxycycline inducible expression of YAP1 wild type cDNA in mammalian cells

    Depositor

    Channing Der

    Article

    Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. mouse E-cadherin GFP (423)

    Plasmid

    #67937

    Purpose

    Mammalian expression of E-cadherin-GFP

    Depositor

    Alpha Yap

    Article

    Truffi et al J Cell Sci. 2014 Jun 1;127(Pt 11):2420-3

    Insert

    E-cadherin (Cdh1 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCherry- SEC61B

    Plasmid

    #121160

    Purpose

    Mammalian expression of mCherry tagged SEC61B, used as a general ER marker

    Depositor

    Christine Mayr

    Article

    Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

    Insert

    SEC61B (SEC61B )
    

    Expression

    Mammalian

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR-ERKKTRClover

    Plasmid

    #59138

    Purpose

    ORF encoding ERK KTR mClover flanked by Gateway sequences

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    ERK Kinase Translocation Reporter (MAPK1 Human, Mouse)
    

    Available Since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. Halo-Sec61-C-18

    Plasmid

    #123285

    Purpose

    Localization: Endoplasmic Reticulum

    Depositor

    Kevin McGowan

    Insert

    Sec61 (SEC61B Human)
    

    Tags

    HaloTag7

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTKRED

    Plasmid

    #41062

    Depositor

    Edward Cox, Thomas Kuhlman

    Article

    Kuhlman et al Nucleic Acids Res. 2010 Apr;38(6):e92. E

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO.1 3xHA-KillerRed-OMP25

    Plasmid

    #174544

    Purpose

    localizes KillerRed to mitochondria

    Depositor

    Minna Roh-Johnson

    Article

    Kidwell et al eLife. 2023 Mar 6;12:e85494. doi: 10.755

    Insert

    3xHA-KillerRed-OMP25
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-BRCA1-mCherry

    Plasmid

    #195275

    Purpose

    express mCherry-BRCA1 fusion

    Depositor

    Xiaokun Shu

    Article

    Li et al Sci Adv. 2023 Mar;9(9):eade3760. doi: 10

    Insert

    mCherry-BRCA1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. TRE-mCherry-p2A-eNpHR3.0-TS

    Plasmid

    #158703

    Purpose

    Reporter construct

    Depositor

    Alice Ting

    Article

    Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

    Insert

    mCherry-p2A-eNpHR3.0-TS
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-Hb9-RFP

    Plasmid

    #37081

    Depositor

    Fred Gage

    Article

    Marchetto et al Cell Stem Cell. 2008 Dec 4;3(6):649-57.

    Insert

    Hb9 RFP
    

    Use

    Lentiviral

    Promoter

    Hb9

    Available Since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTipi2.1_Anti-Strep [IPI-C23.21] Mouse/Rabbit HC

    Plasmid

    #221355

    Purpose

    Mammalian expression of Anti-Strep [IPI-C23.21] Mouse/Rabbit HC; to be used with Anti-Strep [IPI-C23.21] light chain (Plasmid 221356) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-Strep [IPI-C23.21] Mouse/Rabbit HC
    

    Use

    Affinity Reagent/ Antibody

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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