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pCMV-PfV-Sapphire-IRES-DSRed

Plasmid

#116934

Purpose

Mammalian lenti vector for expression of the 40nm GEM and DSRed

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

Pfv

Use

Lentiviral

Tags

Sapphire

Promoter

CMV

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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Eps15-pmCherryN1

Plasmid

#27696

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Eps15 (EPS15 Human)

Tags

mCherry

Expression

Mammalian

Available Since

May 5, 2011

Availability

Academic Institutions and Nonprofits only

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pMito-HyPerGRt

Plasmid

#173197

Purpose

HyPer7 and tdTomato fusion to detect mitochondrial hydrogen peroxide

Depositor

Huiwang Ai

Article

Li et al ACS Sens. 2022 Feb 28. doi: 10.1021/acss

Insert

Hyper7-tdTomato

Expression

Mammalian

Promoter

CMV IE94 promoter

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-DsRed

Plasmid

#11151

Purpose

Mammalian expression vector for expression of DsRed

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Has Service

Cloning Grade DNA

Insert

DsRed2 from Discosoma sp

Expression

Mammalian

Available Since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

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pET-NLS-Cas9-6xHis

Plasmid

#62934

Purpose

Expression of NLS-Cas9-6xHis in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

NLS-Cas9-6xHis

Tags

6xHis tag and NLS

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSYC-437

Plasmid

#163829

Purpose

Foxj1a:GCaMP6s. Fluorescent reporter for calcium signaling (GCaMP6s) driven by zebrafish foxj1a promoter.

Depositor

Seok-Yong Choi

Article

Zhang et al Nat Commun. 2020 Apr 20;11(1):1860. doi:

Inserts

Foxj1a Promoter
GCaMP6s

Use

Multisite gateway destination vector

Available Since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pSIQ

Plasmid

#216238

Purpose

All-in One IQ transgene switch, empty vector

Depositor

Hyunju Ro

Article

Hong et al Mol Ther Methods Clin Dev. 2024 Feb 2;32

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pMRS-XylS-dCas9

Plasmid

#220196

Purpose

mini-Tn7 dCas9 expression vector - XylS/pM promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9

Use

CRISPR

Expression

Bacterial

Mutation

Pseudomonas aeruginosa codon optimized

Promoter

XylS/pM

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF0001

Plasmid

#141469

Purpose

Lentiviral vector for overexpressing the HIF3A transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

HIF3A (HIF3A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-tdPP7-3xmCherry

Plasmid

#74926

Purpose

Expresses tdPP7-3xmCherry

Depositor

Marvin Tanenbaum

Article

Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

Insert

PP7-3xmCherry

Use

Lentiviral

Expression

Mammalian

Available Since

April 29, 2016

Availability

Academic Institutions and Nonprofits only

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pAM5404

Plasmid

#132660

Purpose

RSF1010-based broad host-range plasmid with additional RK2 bom site

Depositor

Susan Golden

Article

Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pTE_20 - pIVT_CMV-synUTR-inactT7-NLS-Cas9-NLS-XTEN-BARD1-NLS-HbaUTR

Plasmid

#252016

Purpose

In vitro transcription of Cas9-BARD1 TruEditor for mRNA generation

Depositor

Neville Sanjana

Article

Burrell et al bioRxiv 2026.02.19.706875

Insert

Cas9-BARD1

Use

CRISPR

Expression

Mammalian

Mutation

NA

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pTE_21 - pIVT_CMV-synUTR-inactT7-NLS-Cas9-NLS-XTEN-BLM(1-193)-NLS-HbaUTR

Plasmid

#252017

Purpose

In vitro transcription of Cas9-BLM(2-194) TruEditor for mRNA generation

Depositor

Neville Sanjana

Article

Burrell et al bioRxiv 2026.02.19.706875

Insert

Cas9-BLM(2-194)

Use

CRISPR

Expression

Mammalian

Mutation

NA

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pTE_22 - pIVT_CMV-synUTR-inactT7-NLS-Cas9-NLS-XTEN-SLX1A-NLS-HbaUTR

Plasmid

#252018

Purpose

In vitro transcription of Cas9-SLX1A TruEditor for mRNA generation

Depositor

Neville Sanjana

Article

Burrell et al bioRxiv 2026.02.19.706875

Insert

Cas9-SLX1A

Use

CRISPR

Expression

Mammalian

Mutation

NA

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Hygro

Plasmid

#127763

Purpose

Used for expression of sgRNA in mammalian cells

Depositor

Ken-Ichi Takemaru

Type

Empty backbone

Expression

Mammalian

Available Since

June 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-PE6a

Plasmid

#207851

Purpose

Mammalian expression of PE6a prime editor

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6a

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Ec48rt(E60K, K87E, E165D, D243N, R267I, E279K, K3…

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FMR1 isoform1-sh1Resistant

Plasmid

#225462

Purpose

Express mEGFP-fusion protein of FMR1 isoform 1

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FMR1 isoform 1 (FMR1 Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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FRP2350

Plasmid

#127580

Purpose

Tagging plasmid for WTC 846: Encodes the PtetO7.1 promoter and Hyg resistance gene

Depositor

Joerg Stelling

Article

Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

Insert

PtetO7.1_Flagtag_8Glycine linker

Use

Synthetic Biology; Pcr based tagging

Tags

Flag tag, 8glycine linker

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pSM-mStayGold

Plasmid

#234317

Purpose

codon-optimized mStayGold with synthetic introns for the expression in C. elegans

Depositor

Kota Mizumoto

Article

Ko et al MicroPubl Biol. 2025 Jan 18;2025:10.1791

Insert

C. elegans codon optimized mStayGOld

Tags

mStayGold

Expression

Worm

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pEvol-NnSULT1C1-cysDNCQ

Plasmid

#188983

Purpose

biosynthesize sulfotyrosine in E. coli.

Depositor

Han Xiao

Article

Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

Inserts

NnSULT1C1
cysD
cysN
cysC
cysQ

Expression

Bacterial

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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