-
Plasmid#186414PurposeAttR3/R4 destination vector for ascidian electroporation with AttB1/B2 recombined NLSLacZ under control of AttB4/B5-recombined pFOG ectodermal regulatory sequence.DepositorInsertlacZ (lacZ E. coli)
UseGateway destination vectorTagsNuclear localization signalExpressionMutationPromoterBasal promoter of the FOG geneAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH333-1-Tier1-PhCMV-dCas9-3xNLS-VP64
Plasmid#169597PurposeTier-1 vector encoding PhCMV-driven dCas9-3xNLS-VP64 expression (PhCMV-dCas9-3xNLS-VP64-pA).DepositorInsertdead S.pyogenes Cas9 - VP64 fusion
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
BPK880-pCAG-DmrC-NLS-3xFLAG-VP64
Plasmid#136912PurposeDmrC with VP64 fused to it's C-terminus; Mammalian expression vectorDepositorInsertpCAG-DmrC-NLS-3xFLAG-VP64
UseCRISPRTagsExpressionMammalianMutationPromoterChicken Beta ActinAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
Gucy1b2-IRES-nlsCre-IRES-taulacZ-FNF TV
Plasmid#105196Purposetargeting vector to generate a Gucy1b2-IRES-nlsCre-IRES-taulacZ mouse strain.DepositorInsertGucy1b2-IRES-nlsCre-IRES-taulacZ-FNF (Gucy1b2 Mouse)
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJM572 ZF6x6-C intC-ZF1-NLS-HA in TUPV2
Plasmid#161559PurposeInducible expression of intC-ZF1-NLS-HA under the ZF6x6-C promoterDepositorInsertintC-ZF1-NLS-HA
UseSynthetic BiologyTagsHAExpressionMammalianMutationPromoterZF6x6-CAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM589 EF1α intN-ZF10-NLS-HA in TUPV2
Plasmid#161565PurposeConstitutive expression of intN-ZF10-NLS-HA under the EF1α promoterDepositorInsertintN-ZF10-NLS-HA
UseSynthetic BiologyTagsHAExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJB004 CMV 3xFLAG-NLS-VP16-intN
Plasmid#161549PurposeConstitutive expression of 3xFLAG-NLS-VP16-intN under the CMV promoterDepositorInsert3xFLAG-NLS-VP16-intN
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJB005 CMV 3xFLAG-NLS-intC-ZF2
Plasmid#161550PurposeConstitutive expression of 3xFLAG-NLS-intC-ZF2 under the CMV promoterDepositorInsert3xFLAG-NLS-intC-ZF2
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM516 EF1α intC-ZF1-NLS-HA in TUPV2
Plasmid#161551PurposeConstitutive expression of intC-ZF1-NLS-HA under the EF1α promoterDepositorInsertintC-ZF1-NLS-HA
UseSynthetic BiologyTagsHAExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM522 EF1α intC-ZF10-NLS-HA in TUPV2
Plasmid#161552PurposeConstitutive expression of intC-ZF10-NLS-HA under the EF1α promoterDepositorInsertintC-ZF10-NLS-HA
UseSynthetic BiologyTagsHAExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJB002 CMV 3xFLAG-NLS-DsDed-ZF1
Plasmid#161532PurposeConstitutive expression ofCMV 3xFLAG-NLS-DsDed-ZF1 under the CMV promoterDepositorInsert3xFLAG-NLS-DsDed-ZF1
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM503 EF1α 3xFLAG-NLS-ZF1 in TUPV2
Plasmid#161534PurposeConstitutive expression of 3xFLAG-NLS-ZF1 under the EF1α promoterDepositorInsert3xFLAG-NLS-ZF1
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-synapsin-NLS-mTurquoise2-optoNES-WPRE
Plasmid#159941Purposeoptical regulation of nuclear exportDepositorInsertNLS-mTurquoise2-optoNES
UseAdenoviralTagsExpressionMammalianMutationsyntheticPromoterhSyn1Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-mScarlet-3XNLS-optoNES-WPRE
Plasmid#159948Purposeoptical regulation of nuclear exportDepositorInsertmScarlet-3XNLS-optoNES
UseAdenoviralTagsExpressionMammalianMutationsyntheticPromoterhSyn1Available sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-YPET-3XNLS-optoNES-WPRE
Plasmid#159947Purposeoptical regulation of nuclear exportDepositorInsertYPET-3XNLS-optoNES
UseAdenoviralTagsExpressionMammalianMutationsyntheticPromoterhSyn1Available sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-mTFP1.0-optoNES-WPRE
Plasmid#159945Purposeoptical regulation of nuclear exportDepositorInsertNLS-mTFP1.0-optoNES
UseAdenoviralTagsExpressionMammalianMutationsyntheticPromoterhSyn1Available sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-YPET-optoNES-WPRE
Plasmid#159942Purposeoptical regulation of nuclear exportDepositorInsertNLS-YPET-optoNES
UseAdenoviralTagsExpressionMammalianMutationsyntheticPromoterhSyn1Available sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-mScarlet-optoNES-WPRE
Plasmid#159943Purposeoptical regulation of nuclear exportDepositorInsertNLS-mScarlet-optoNES
UseAdenoviralTagsExpressionMammalianMutationsyntheticPromoterhSyn1Available sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-Dronpa-optoNES-WPRE
Plasmid#159944Purposeoptical regulation of nuclear exportDepositorInsertNLS-Dronpa-optoNES
UseAdenoviralTagsExpressionMammalianMutationsyntheticPromoterhSyn1Available sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-mTurquoise2-3XNLS-optoNES-WPRE
Plasmid#159946Purposeoptical regulation of nuclear exportDepositorInsertmTurquoise2-3XNLS-optoNES
UseAdenoviralTagsExpressionMammalianMutationsyntheticPromoterhSyn1Available sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-YPET-optoNES.F434L-WPRE
Plasmid#159975Purposeoptical regulation of nuclear export with shorter relaxation timeDepositorInsertNLS-YPET-optoNES.F434L
UseAdenoviralTagsExpressionMammalianMutationF434LPromoterhSyn1Available sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-YPET-optoNES.V416I-WPRE
Plasmid#159973Purposeoptical regulation of nuclear export with extended relaxation timeDepositorInsertNLS-YPET-optoNES.V416I
UseAdenoviralTagsExpressionMammalianMutationV416IPromoterhSyn1Available sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-YPET-optoNES.I427V-WPRE
Plasmid#159974Purposeoptical regulation of nuclear export with shorter relaxation timeDepositorInsertNLS-YPET-optoNES.I427V
UseAdenoviralTagsExpressionMammalianMutationI427VPromoterhSyn1Available sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-HOXD13(-7A)-IDR-mCherry-CRY2-NLS
Plasmid#145271PurposeMammalian Expression of Nuclear Opto HOXD13(-7A) IDR with SV40 NLSDepositorInsertHOXD13(-7A)-IDR (HOXD13 Human)
UseTagsExpressionMammalianMutationA-repeat deletedPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-HOXA13(+7A)-IDR-mCherry-CRY2-NLS
Plasmid#145266PurposeMammalian Expression of Nuclear Opto HOXA13(+7A) IDR with SV40 NLSDepositorInsertHOXA13(+7A)-IDR (HOXA13 Human)
UseTagsExpressionMammalianMutationA-repeat expandedPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-HOXA13-IDR-mCherry-CRY2-NLS
Plasmid#145265PurposeMammalian Expression of Nuclear Opto HOXA13 IDR with SV40 NLSDepositorInsertHOXA13wt-IDR (HOXA13 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-HOXD13(+8A)-IDR-mCherry-CRY2-NLS
Plasmid#145262PurposeMammalian Expression of Nuclear Opto HOXD13(+8A) IDR with SV40 NLSDepositorInsertHOXD13(+8A)-IDR (HOXD13 Human)
UseTagsExpressionMammalianMutationA-repeat expandedPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-VALENTIA-016 NLSNoLS-Sirius//BsaI
Plasmid#130197PurposeEMMA Kit # 1000000119 extensionDepositorInsertNLSNoLS-Sirius
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-VALENTIA-017 NLSNoLS-SuperNova//BsaI
Plasmid#130198PurposeEMMA Kit # 1000000119 extensionDepositorInsertNLSNoLS-SuperNova
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-VALENTIA-033 NLSNoLS-Eos//BsaI
Plasmid#130214PurposeEMMA Kit # 1000000119 extensionDepositorInsertNLSNoLS-Eos
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA1(CTD)-NLS(SV40) (KAC438)
Plasmid#133796PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA1 (truncated C-terminal domain only) with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA1 (truncated C-terminal domain only)
UseTagsNLS(SV40)ExpressionMammalianMutationC-terminal domain onlyPromoterCMV and T7Available sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA1(T16A)-NLS(SV40) (KAC511)
Plasmid#133797PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA1(T16A) with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA1(T16A)
UseTagsNLS(SV40)ExpressionMammalianMutationT16APromoterCMV and T7Available sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA1-Leu-NLS(SV40) (KAC440)
Plasmid#133799PurposeCMV and T7 promoter expression plasmid for human codon optimized Leu-AcrIIA1 with C-terminal NLS (SV40)DepositorInserthuman codon optimized Leu-AcrIIA1
UseTagsNLS(SV40)ExpressionMammalianMutationn/aPromoterCMV and T7Available sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA1-Eriv-NLS(SV40) (KAC442)
Plasmid#133800PurposeCMV and T7 promoter expression plasmid for human codon optimized Eriv-AcrIIA1 with C-terminal NLS (SV40)DepositorInserthuman codon optimized Eriv-AcrIIA1
UseTagsNLS(SV40)ExpressionMammalianMutationn/aPromoterCMV and T7Available sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8637 PB-PGK-C intein-2XNLS-miniVPR-mCherry-pA
Plasmid#140224PurposeConstitutive PGK driven expression of intein with miniVPR for 3-way split activatorDepositorInsertC-intein with miniVPR
UseTagsmCherryExpressionMammalianMutationPromoterPGKAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-VALENTIA-030 NLSNoLS-mVenus//BsaI
Plasmid#130211PurposeEMMA Kit # 1000000119 extensionDepositorInsertNLSNoLS-mVenus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-VALENTIA-020 NLSNoLS-Neptune//BsaI
Plasmid#130201PurposeEMMA Kit # 1000000119 extensionDepositorInsertNLSNoLS-Neptune
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-VALENTIA-018 NLSNoLS-Eos//BsaI
Plasmid#130199PurposeEMMA Kit # 1000000119 extensionDepositorInsertNLSNoLS-Eos
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
phage - ubc - nls - 2xmcp - egfp - cMycBirA*
Plasmid#131133PurposeBirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.DepositorInsertNLS-HA-2xMCP-EGFP-cMycBirA*
UseLentiviralTagsNLS, HA, cMyc (N-terminal of BirA*)ExpressionMammalianMutationBirA* = BIrA >R118GPromoterhuman ubiquitin C (UBC)Available sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3- myc NES-mCE(K294A) NLS
Plasmid#82467PurposeExpresses cytoplasmic restricted form of myc tagged mRNA capping enzymeDepositorInsertNLS deleted from mouse Capping Enzyme (Rngtt Mouse)
UseTagsExpressionMammalianMutationdeletion of KRKY from 573-657PromoterCMVAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only