We narrowed to 6,530 results for: nls
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Plasmid#132543PurposeExpresses CIRTS-1 (ORF5-TBP6.7-Pin domain-NLS) in mammalian cellsDepositorInsertCIRTS-1: ORF5-TBP6.7-Pin domain-NLS
TagsFLAG tagExpressionMammalianPromotercmv d0Available SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FRB-NLS-3HA-HIS3MX6
Plasmid#228023PurposeTo integrate TID-FRB-NLS fusion at yeast promoter; yeast BioID with HIS3MX6 markerDepositorInsertTurboID-FRB
TagsHA tagExpressionYeastAvailable SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-IRES-3xNLS-mScarlet-I3-pA (JDW 1360)
Plasmid#224536PurposeA Gateway compatible 3' entry clone containing IRES-FLAG- NLS-mScarlet-I3DepositorInsertIRES-FLAG- NLS-mScarlet-I3
UseGateway CloningAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA
Plasmid#192552PurposeExpresses nuclear localized tdTomato under CAG promoterDepositorHas ServiceAAV PHP.eB and AAV9InserttdTomato
UseAAVTagsNLSMutationdeleted E273 and G274 compared to FPbase ID: PGG5SPromoterCAGAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST1374-N-NLS-flag-linker-Cas9-H840A
Plasmid#51129PurposeCas9 nickase optimized for nuclear import.DepositorInsertCas9 nickase
UseCRISPRTagsFlag and NLSExpressionMammalianMutationH840APromoterCMVAvailable SinceMarch 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-P(Cry1)-DIO-intron336-Venus-NLS-D2
Plasmid#110055PurposeCry1 transcription fluorescence reporterDepositorInsertCry1 promoter and Venus (Cry1 Mouse)
UseAAV and Cre/LoxTagsNLS-D2ExpressionMammalianPromoterCry1Available SinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-P(Per2)-DIO-intron2-Venus-NLS-D2
Plasmid#110057PurposePer2 transcription fluorescence reporterDepositorInsertPer2 promoter and Venus (Per2 Mouse)
UseAAV and Cre/LoxTagsNLS-D2ExpressionMammalianPromoterPer2Available SinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn1-GCaMP6f-P2A-nls-dTomato
Plasmid#51085PurposeNeuronal expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophoreDepositorHas ServiceAAV Retrograde and AAV1InsertGCaMP6f
UseAAVTagsP2A-nls-dTomatoExpressionMammalianPromoterhuman Synapsin1Available SinceFeb. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICE-RNaseHI-D10R-E48R-NLS-mCherry
Plasmid#60367PurposePlasmid for expression of an inactive mutant of bacterial RNase HI tagged with NLS-mCherry that can be used to detect R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.DepositorInsertRNase HI-D10R-E48R
TagsNLS from SV40 T antigen and mCherryExpressionMammalianMutationD10R+E48R; catalyticaly inactivePromoterCMV-tetAvailable SinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-sTomato-NLS. PGK-puro
Plasmid#136261PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagssTomato-NLSExpressionMammalianAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSH-EFIRES-P-AtAFB2-mCherry-weak NLS
Plasmid#129717PurposeAAVS1 safe harbor targeting vector expressing AtAFB2-mCherry-weak NLSDepositorInsertAFB2 (AFB2 Mustard Weed)
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsmCherry-weak NLSExpressionMammalianMutationcodon optimizedPromoterEF1aAvailable SinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPV-Dual_promoter-EF1α-2xNLS-Cascade+Cas3-P (RD)
Plasmid#204619PurposeExpression vector of Cascade and Cas3. Puromycin selectable.DepositorInsertCas7, Cas5, Cas8, Cas11, Cas6, and Cas3
UseCRISPRTagsEach protein has C- and N-terminal SV40 NLSPromoterEF1aAvailable SinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAY19 (OsTIR1(F74A) mAID-EGFP-NLS)
Plasmid#140535PurposeOsTIR1(F74A)-P2A-mAID-EGFP-NLS TOL2DepositorInsertmAID-EGFP-NLS TOL2
ExpressionMammalianAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8547 PB-PGK-dLbCpf1-2XNLS-miniVPR-mCherry-pA
Plasmid#140220PurposeConstitutive PGK driven expression of full length dCpf1 (Cas12a) -miniVPR activatorDepositorInsertdLbCpf1
UseCRISPRTagsminiVPR-mCherryExpressionMammalianMutationD832APromoterPGKAvailable SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-3*Flag-APEX2 C-term 3NLS
Plasmid#187588PurposeExpress FLAG epitope and APEX2-tagged 3xNLS fusion protein in mammalian cellsDepositorInsertNLS
TagsAPEX2-FLAGExpressionMammalianPromoterCMVAvailable SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS305-LEU2-pINO4-SV40NLS-PfV-GS-Sapphire
Plasmid#203655PurposeYeast integrative vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the INO4 promoter.DepositorArticleInsertPfV
TagsSapphireExpressionYeastAvailable SinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS403-HIS3-pINO4-SV40NLS-PfV-GS-Sapphire
Plasmid#203658PurposeYeast integrative vector for the expression of the Sapphire-tagged nuclear localizing 40nm GEMs under the HIS3 promoter.DepositorArticleInsertPfV
TagsSapphireExpressionYeastAvailable SinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-CaMKIIa-GCaMP6s-P2A-nls-dTomato
Plasmid#51086PurposeForebrain principle neuron specific expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophoreDepositorInsertGCaMP6s
UseAAVTagsP2A-nls-dTomatoExpressionMammalianPromoterCaMKIIa 1.3 kbAvailable SinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-V5-3xHA-miniTurbo-3XNLS-STOP_IDG-K
Plasmid#158160PurposeGateway destination clone of miniTurbo-3XNLS (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertV5-3xHA-miniTurbo-3XNLS
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianPromoterUbiquitinAvailable SinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-mNeonGreen-NLS. PGK-puro
Plasmid#136262PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagsmNeonGreen-NLSExpressionMammalianAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS305-LEU2-pINO4-SV40NLS-PfV-GS-yEmCherry
Plasmid#203656PurposeYeast integrative vector for the expression of the yEmCherry-tagged nuclear localizing 40nm nucGEMs under the INO4 promoter.DepositorArticleInsertPfV
TagsyEmCherryExpressionYeastAvailable SinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-PGKneo-EF1a-NLS-MARIO-SV40 pA
Plasmid#247646Purposemammalian expression of NLS-MARIO, a genetically-encoded fluorescent Mg2+ sensorDepositorInsertMARIO
TagsNLSExpressionMammalianAvailable SinceNov. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-EGFP-NLS-P2AT2A-mCherry-PTS1
Plasmid#87828PurposeHigh-efficient mammalian expression vector for co-expression of EGFP-tagged and mCherry-tagged proteins using P2AT2A. NLS and PTS1 can be replaced by proteins of interest.DepositorInsertsNLS
PTS1
TagsEGFP and mCherryExpressionMammalianPromoterCMV promoter, tetracycline operatorAvailable SinceApril 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
PHY23-phage-ubc-nls-ha-stdMCP-tdTomato
Plasmid#164044PurposeExpression of stdMCP-tdTomato in mammalian cellsDepositorInsertstdMCP-tdTomato
UseLentiviralExpressionMammalianPromoterhuman ubiquitin C (UBC)Available SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-mScarletI-NLS. PGK-puro
Plasmid#136263PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagsmScarletI-NLSExpressionMammalianAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-mCherry-P2A-NLSFlpN-nMagHigh
Plasmid#180585PurposePA-FlpDepositorInsertSplit FlpO
UseAAVAvailable SinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-CaMKIIa-GCaMP6f-P2A-nls-dTomato
Plasmid#51087PurposeForebrain principle neuron specific expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophoreDepositorInsertGCaMP6f
UseAAVTagsP2A-nls-dTomatoExpressionMammalianPromoterCaMKIIa 1.3 kbAvailable SinceFeb. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pL6puro-JeT-pmRevErbA-mClover3-NLS-Pest
Plasmid#240109PurposeA lentiviral (yellow-green) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by synthtic JeT promoter.DepositorInsertmurine Nr1d1 promoter+mClover3-NLS-Pest-Reporter (Nr1d1 Mouse)
UseLentiviralMutationadded JeT PromoterAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEG302 22aa SunTag NtDRMcd (noNLS) nog
Plasmid#119554PurposeCRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to specific loci of interest (nog=no guide)DepositorInsertNOS_NLS_GB1_noNLS_linker_DRMcd_linker sfGFP_scFv_UBQ10_Insulator UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMVd1-NLS-GAL4-iLight-VP16
Plasmid#219549PurposeNLS-Gal4(1-65)-iLight-VP16 construc expression in mammalian cells. pAAV vector.DepositorInsertNLS-Gal4(1-65)-iLight-VP16
UseAAVExpressionMammalianPromoterCMVd1Available SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hSt3Cas9-NLS(SV40)-3xFLAG (KAC426)
Plasmid#133788PurposeCAG promoter expression plasmid for human codon optimized St3Cas9 nuclease with C-terminal NLS (SV40)DepositorInserthuman codon optimized St3Cas9
TagsNLS(SV40)-3xFLAGExpressionMammalianMutationn/aPromoterCAGAvailable SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-P(Cry1)-forward-intron336-Venus-NLS-D2
Plasmid#110054PurposeCry1 transcription fluorescence reporterDepositorAvailable SinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pL6puro-JeT-pmRevErbA-Electra2-NLS-Pest
Plasmid#240111PurposeA lentiviral (blue) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by synthtic JeT promoter.DepositorInsertmurine Nr1d1 promoter+Electra2-NLS-Pest-Reporter (Nr1d1 Mouse)
UseLentiviralMutationadded JeT PromoterAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
Pub-mCD8:GFP-T2A-DsRed:NLS-SV40
Plasmid#130665PurposePlasmid to test the efficiency of T2A multiplexing in Aedes aegyptiDepositorInsertAaegPUb-mCD8-GFP-T2A-dsRed-NLS-SV40
UseAe. aegypti expressionExpressionInsectPromoterAedes aegypti poly-ubiquitinAvailable SinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pST1374-N-NLS-flag-linker-Cas9-D10A
Plasmid#51130PurposeCas9 nickase optimized for nuclear import.DepositorInsertCas9 nickase
UseCRISPRTagsFlag and NLSExpressionMammalianMutationD10A (nickase mutant)PromoterCMVAvailable SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-eGFP-NLS-RNF168 delta RING
Plasmid#133983Purposemammalian expression vector of eGFP tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)DepositorInsertRNF168 (RNF168 Human)
TagseGFPExpressionMammalianMutationdeletion of RING; siRNA resistant sequence: GAGGA…PromoterCMVAvailable SinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
UAST-FRT-GFP-FRT-uMCas12a+nls1x
Plasmid#230908PurposeDouble inducible expression of Cas12a+ with Gal4 and Flp.DepositorInsertCas12a+
UseCRISPRExpressionInsectAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
Col1A2:TFPnls-T2a-mErt-Cre-Ert
Plasmid#111151PurposeFibroblasts specific inducible CreDepositorInsertTFPnls-T2a-mErt-Cre-Ert
UseCre/LoxMutationwtPromoterCol1A2Available SinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-SpRY-HF1-NLS-6xHis (gbr2102)
Plasmid#181744Purposebacterial expression plasmid for SpRY-HF1 with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tagDepositorInsertbacterial codon optimized SpRY-HF1
UseIn vitro transcription; t7 promoterTagsBPNLS(SV40)-6xHis and MKIEE MBP tagExpressionBacterialMutationSpRY-HF1, SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E…PromoterT7Available SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-ChroME2s-ST-P2A-NLS-mRuby3
Plasmid#170177PurposeCation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vectorDepositorInsertChroME2s-ST-P2A-NLS-mRuby3
UseAAVExpressionMammalianAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only