We narrowed to 52,555 results for: son
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Plasmid#133754PurposeLentiviral expression of human TDP-43 A90V disease mutant in mammalian cells.DepositorInsertTARDBP (TARDBP Human)
UseLentiviralExpressionMammalianMutationChanged alanine 90 to valinePromoterCMVAvailable SinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Chr8q_Centromere-Targeting_gRNA
Plasmid#195128PurposegRNA targeting centromere-proximal location on Chromosome 8q in a third generation Cas9 backbone with GFPDepositorInsertChr8q gRNA
ExpressionMammalianAvailable SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-TDP-43-P112H
Plasmid#133760PurposeLentiviral expression of human TDP-43 P112H disease mutant in mammalian cells.DepositorInsertTDP-43 (TARDBP Human)
UseLentiviralExpressionMammalianMutationChanged proline 112 to histidine.PromoterCMVAvailable SinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
Rosa26_gRNA
Plasmid#196138PurposegRNA targeting the Rosa26 locus in a thid generation Cas9 backbone with TagBFP2DepositorInsertRosa26 gRNA
UseCRISPR and LentiviralExpressionMammalianAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-TDP-43-D169G
Plasmid#133757PurposeLentiviral expression of human TDP-43 D169G disease mutant in mammalian cells.DepositorInsertTDP-43 (TARDBP Human)
UseLentiviralExpressionMammalianMutationChanged aspartate 169 to glycine.PromoterCMVAvailable SinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
OR6K6_Deletion_gRNA2
Plasmid#195196Purposedual gRNAs for deletion of OR6K6 in a third generation Cas9 backbone with GFPDepositorInsertOR6K6 dual gRNA (OR6K6 Human)
ExpressionMammalianAvailable SinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
OR6K6_Deletion_gRNA1
Plasmid#195195Purposedual gRNAs for deletion of OR6K6 in a third generation Cas9 backbone with GFPDepositorInsertOR6K6 dual gRNA (OR6K6 Human)
ExpressionMammalianAvailable SinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
OR6K6_Deletion_gRNA3
Plasmid#195197Purposedual gRNAs for deletion of OR6K6 in a third generation Cas9 backbone with GFPDepositorInsertOR6K6 dual gRNA (OR6K6 Human)
ExpressionMammalianAvailable SinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-luciferase-reporter-POFUT2-SVA
Plasmid#202831PurposepGL3 luciferase reporter with POFUT2 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expressionDepositorInsertPOFUT2 intronic SVA
UseLuciferaseExpressionMammalianPromoterSV40Available SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-luciferase-reporter-HTT-SVA
Plasmid#202833PurposepGL3 luciferase reporter with HTT SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expressionDepositorInsertHTT intronic SVA
UseLuciferaseExpressionMammalianPromoterSV40Available SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-luciferase-reporter-CHAF1B-SVA
Plasmid#202829PurposepGL3 luciferase reporter with CHAF1B SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expressionDepositorInsertCHAF1B intronic SVA
UseLuciferaseExpressionMammalianPromoterSV40Available SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EML4-ALK variant 3a I1171N/D1203N
Plasmid#183833PurposeExpress EML4-ALK fusion variant 3a (E6a;A20) harboring ALK I1171N/D1203N mutation in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of EML4 exon 1-6a and ALK exon…ExpressionMammalianMutationI1171N/D1203NPromoterCMVAvailable SinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
TetO-CDK5RAP2
Plasmid#202840PurposeDoxycycline inducible expression of CDK5RAP2 cDNADepositorAvailable SinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2-sgRNAs-del-ZNF91-BFP
Plasmid#202823PurposeLentiviral vector modified to express two sgRNAs that delete exon 1 within the ZNF91 gene with EF-1apha for Cas9 and BFP expressionDepositorInserttwo sgRNAs (each with a U6 promoter) to delete exon 1 within ZNF91 gene
UseLentiviralExpressionMammalianPromoterU6 - twoAvailable SinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2-sgRNAs-del-ZNF91-mcherry
Plasmid#202822PurposeLentiviral vector modified to express two sgRNAs that delete exon 1 within the ZNF91 gene with EF-1apha for Cas9 and mcherry expressionDepositorInserttwo sgRNAs that delete exon 1 within the ZNF91 gene
UseLentiviralExpressionMammalianPromoterU6 - twoAvailable SinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-5'-aptamer-tagged-SVA-lncRNA-AK057321-mcherry
Plasmid#202818PurposeLentiviral vector to express 5'-aptamer tagged SVA-lncRNA AK057321 with CMV for mcherry expressionDepositorInsertSVA-lncRNA AK057321
UseLentiviralExpressionMammalianMutationaddition of a dual aptamer RNA tag 5' to the…PromoterU6Available SinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-EFalpha-SVA-lncRNA-AK057321-mcherry-puro
Plasmid#202800PurposeLentiviral vector to express SVA-lncRNA AK057321 with ires for mcherry and puro expressionDepositorInsertSVA-lncRNA AK057321
UseLentiviralExpressionMammalianPromoterEFalpha (with intron)Available SinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO GFP11-LRRK2
Plasmid#231174PurposeExpresses GFP-11-LRRK2 (wildtype, full length) in mammalian cellsDepositorAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Chr1q_Cassette-Integration_gRNA
Plasmid#195126PurposegRNA in a third generation Cas9 vector with GFP, targeting location downstream of Chr1q_centromere-targeting_gRNA for positive-negative selection cassette integrationDepositorInsertChr1q gRNA
ExpressionMammalianAvailable SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Orf9b
Plasmid#156485PurposeBacterial expression of Sars-CoV2 Orf9b protein with His-tag and GST-tagDepositorInsertOrf9b
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
OR2M4_Deletion_gRNA
Plasmid#195194Purposedual gRNAs for deletion of OR2M4 in a third generation Cas9 backbone with GFPDepositorInsertOR2M4 dual gRNA (OR2M4 Human)
ExpressionMammalianAvailable SinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Control-cx43.4-48H-pPRISM
Plasmid#117801PurposeControl to test technique on a target that is known to workDepositorInsertTagRFP
UseSynthetic BiologyAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9 (BB)-2A-GFP-RB1sg
Plasmid#229857Purposesingle guide RNA targeting human RB1 geneDepositorInsertRB1sgRNA (RB1 Human)
TagsThe gamma-tubulin sgRNA is coexpressed with 3xFLA…ExpressionMammalianAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL3-luciferase-reporter-CDK5RAP2-SVA
Plasmid#202828PurposepGL3 luciferase reporter with CDK5RAP2 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expressionDepositorInsertintronic SVA within CDK5RAP2 gene
UseLuciferaseExpressionMammalianPromoterSV40Available SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-luciferase-reporter-AGPAT3-SVA
Plasmid#202827PurposepGL3 luciferase reporter with AGPAT3 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expressionDepositorInsertintronic SVA located in AGPAT3 gene
UseLuciferaseExpressionMammalianPromoterSV40Available SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
Chr1q_Cassette-Proximal_gRNA
Plasmid#195127PurposegRNA in a third generation Cas9 vector with GFP, targeting location immediately downstream of Chr1q_cassette-integration_gRNA, to be used for cassette deletion controlsDepositorInsertChr1q gRNA
ExpressionMammalianAvailable SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp3c_SUD-C
Plasmid#156471PurposeBacterial expression of Sars-CoV2 Nsp3c_SUD-C protein with His-tag and GST-tagDepositorInsertNsp3c_SUD-C (ORF1ab Synthetic)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJC20cdc8.27
Plasmid#99362PurposeBacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.27.DepositorInsertcdc8 (cdc8 Fission Yeast)
ExpressionBacterialMutationGlutamic acid 129 to LysinePromoterT7Available SinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCJ136
Plasmid#162665PurposepET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
TagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp16
Plasmid#156482PurposeBacterial expression of Sars-CoV2 Nsp16 protein with His-tag and GST-tagDepositorInsertNsp16 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2-sgRNAs-del-CDK5RAP2-SVA-BFP
Plasmid#202824PurposeLentiviral vector modified to express two sgRNAs that delete the intronic SVA within the CDK5RAP2 gene with EF-1apha for Cas9 and BFP expressionDepositorInserttwo sgRNAs (each with a U6 promoter) to delete SVA within CDK5RAP2 gene
UseLentiviralExpressionMammalianPromoterU6 - twoAvailable SinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2-sgRNAs-del-SCN8A-SVA-BFP
Plasmid#202826PurposeLentiviral vector modified to express two sgRNAs that delete the intronic SVA within the SCN8A gene with EF-1apha for Cas9 and BFP expressionDepositorInserttwo sgRNAs (each with a U6 promoter) to delete intronic SVA within SCN8A gene
UseLentiviralExpressionMammalianPromoterU6 - twoAvailable SinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2-sgRNAs-del-AK057321-SVA-mcherry
Plasmid#202821PurposeLentiviral vector modified to express two sgRNAs that delete the SVA within the SVA-lncRNA AK057321 gene (exon 3) with EF-1apha for Cas9 and mcherry expressionDepositorInserttwo sgRNAs (each with a U6 promoter) to delete SVA within SVA-lncRNA AK057321 gene
UseLentiviralExpressionMammalianPromoterU6 (two)Available SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2-sgRNAs-del-CHAF1B-SVA-BFP
Plasmid#202825PurposeLentiviral vector modified to express two sgRNAs that delete the intronic SVA within the CHAF1B gene with EF-1apha for Cas9 and BFP expressionDepositorInserttwo sgRNAs (each with a U6 promoter) to delete intronic SVA within CHAF1B gene
UseLentiviralExpressionMammalianPromoterU6 - twoAvailable SinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
TP53-KO_gRNA_1
Plasmid#195130PurposeTP53-targeting gRNA in the LRG2.1 backboneDepositorInsertTP53 KO gRNA 1 (TP53 Human)
ExpressionMammalianAvailable SinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp5_Mpro
Plasmid#156475PurposeBacterial expression of Sars-CoV2 Nsp5_Mpro protein with His-tag and GST-tagDepositorInsertNsp5_Mpro (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTER RB1shRNA human
Plasmid#66886PurposeDoxycycline-regulated mammalian expression vector for expressing shRNA against RB1DepositorAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSCVneo-DEST
Plasmid#119746PurposeAllows Gateway cloning of gene of interest into retroviral pMSCV vector (with Neomycin/G418 resistance)DepositorTypeEmpty backboneUseRetroviralExpressionMammalianAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp10
Plasmid#156479PurposeBacterial expression of Sars-CoV2 Nsp10 protein with His-tag and GST-tagDepositorInsertNsp10 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
TP53-KO_gRNA_2
Plasmid#195131PurposeTP53-targeting gRNA in the LRG2.1 backboneDepositorInsertTP53 KO gRNA 2 (TP53 Human)
ExpressionMammalianAvailable SinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only