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We narrowed to 4,645 results for: PEP

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Showing: 1901 - 1950 of 4645 results

  1. mRuby-ER-5

    Plasmid
    #55860
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 558, Emission: 605
    Depositor
    Michael Davidson
    Insert
    ER (CALR Human)
    Tags
    mRuby
    Expression
    Mammalian
    Mutation
    ER Signal Peptide
    Promoter
    CMV
    Available Since
    March 12, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    55860-plasmid-map-sequence-id-174991

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  2. mVenus-ER-5

    Plasmid
    #56611
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 515, Emission: 528
    Depositor
    Michael Davidson
    Insert
    ER (CALR Human)
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    ER Signal Peptide
    Promoter
    CMV
    Available Since
    Dec. 18, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. mCitrine-ER-5

    Plasmid
    #56557
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 516, Emission: 529
    Depositor
    Michael Davidson
    Insert
    ER (CALR Human)
    Tags
    mCitrine
    Expression
    Mammalian
    Mutation
    ER Signal Peptide
    Promoter
    CMV
    Available Since
    Dec. 5, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. CMV-mt4xi-GCaMP6f

    Plasmid
    #192075
    Purpose
    Fluorescent reporter for mitochondrial calcium
    Depositor
    Timothy Ryan
    Insert
    4 repeats of improved COX8 signal peptide plus GCaMP6f
    Expression
    Mammalian
    Available Since
    Dec. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    192075-plasmid-map-sequence-id-378919

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  7. pKB223

    Plasmid
    #170013
    Purpose
    Encodes LacI-controlled expression of surface-displayed C18G and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    C18G
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Available Since
    Aug. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    170013-plasmid-map-sequence-id-335942

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  13. pAAV hPGRN

    Plasmid
    #213682
    Purpose
    Expresses human progranulin (PGRN) with an N-terminal twin-Strep-V5 tag
    Depositor
    Thomas Kukar
    Article
    Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi
    Insert
    Human Progranulin (hPGRN) (GRN Human)
    Use
    AAV
    Tags
    Twin-Strep tag and V5 tag (after signal peptide)
    Promoter
    cytomegalovirus enhancer/chicken β-actin promoter
    Available Since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    213682-plasmid-map-sequence-id-455751

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  15. pHN872

    Plasmid
    #178830
    Purpose
    Bacterial expression for human Saposin A
    Depositor
    Kazuhiro Abe
    Insert
    artificial gene encoding human Saposin A
    Tags
    GST, His8, TEV
    Expression
    Bacterial
    Mutation
    Codon-usage was optimized for bacterial expressio…
    Promoter
    T7
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178830-plasmid-map-sequence-id-351931

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  16. p2Bac.V5.p62

    Plasmid
    #39218
    Depositor
    Aziz Sancar
    Insert
    p62 (GTF2H1 Human)
    Tags
    V5
    Expression
    Insect
    Promoter
    Pp10
    Available Since
    Aug. 24, 2012
    Availability
    Academic Institutions and Nonprofits only

  18. pFP.R270

    Plasmid
    #138219
    Purpose
    Expresses the split mCherry reporter interrupted by the IMPDH-1 intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(GGG).IMPDH-1.(SIC)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138219-plasmid-map-sequence-id-273501

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  19. pFP.R272

    Plasmid
    #138221
    Purpose
    Expresses the split mCherry reporter interrupted by the SspGyrB artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(AGG).SspGyrB.(SAK)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138221-plasmid-map-sequence-id-273511

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  21. pET28a-HsSENP5

    Plasmid
    #71468
    Purpose
    Encodes human full length SENP5 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).
    Depositor
    Jorge Eduardo Azevedo
    Article
    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:
    Insert
    SUMO1/sentrin specific peptidase 5 (SENP5 Human)
    Tags
    Hexahistidine
    Expression
    Bacterial
    Mutation
    Gene optimised for E. coli expression (Genscript)
    Promoter
    T7
    Available Since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  22. pBJKS-mPar4

    Plasmid
    #53232
    Purpose
    mammalian expression of mouse Par4
    Depositor
    Shaun Coughlin
    Insert
    Par4 (F2rl3 Mouse)
    Tags
    FLAG and bovine prolactin signal sequence (MDSKGS…
    Expression
    Mammalian
    Mutation
    Deletion of aa1-17 (native signal peptide)
    Available Since
    Aug. 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  23. USP15

    Plasmid
    #28282
    Depositor
    Cheryl Arrowsmith
    Insert
    USP15 (USP15 Human)
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    Contains dusp and Ubl domains (residues 6-223).
    Available Since
    April 14, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  24. KNS2

    Plasmid
    #28122
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    KNS2:HPC09H-C05:C207000 (KLC1 Human)
    Tags
    His tag with TEV site
    Expression
    Bacterial
    Available Since
    March 22, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  27. pMT-flk1-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80056
    Purpose
    Flk1/Kdrl promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    flk1
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80056-plasmid-map-sequence-id-151372

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  29. pcDNA3-sACE2v5-sfGFP

    Plasmid
    #145176
    Purpose
    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 5
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Linker GSGGSGSGG and superfolder GFP
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available Since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    145176-plasmid-map-sequence-id-292575

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  30. pcDNA3-sACE2v4-sfGFP

    Plasmid
    #145175
    Purpose
    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 4
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Linker GSGGSGSGG and superfolder GFP
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available Since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    145175-plasmid-map-sequence-id-292620

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  39. DPP7 (3N0T)

    Plasmid
    #28114
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DPP7:SDC134-D09:C41107 (DPP7 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    contains amino acid residues 28-492
    Available Since
    March 18, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  44. pMT-MCS-NLS-BirA-2A-mCherry_Ras

    Plasmid
    #80059
    Purpose
    MCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80059-plasmid-map-sequence-id-152593

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  45. pMT-ubb-NLS-BirA-2a-mCherry

    Plasmid
    #79886
    Purpose
    Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    Ubiquitin
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79886-plasmid-map-sequence-id-150062

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  47. p11_AaC60αβ/C20

    Plasmid
    #229506
    Purpose
    Expresses Anthoceros agrestis Chaperonin 60α, Chaperonin 60β, Chaperonin 20
    Depositor
    Laura Gunn
    Article
    Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.
    Inserts
    Chaperonin 60α
    Chaperonin 60β
    Chaperonin 20
    Expression
    Bacterial
    Mutation
    N terminus chloroplast transit peptide truncation
    Promoter
    T7
    Available Since
    June 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    229506-plasmid-map-sequence-id-456756

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  48. pB1H2 UV5 Zif268-5ala (2-hybrid bait plasmid)

    Plasmid
    #128165
    Purpose
    UV5 promoter drives expression of Zif268-5alanine peptide fusion. Pair with pGHUC w-ERBIN as a negative control (doesn't turn on HIS3/GFP).
    Depositor
    Marcus Noyes
    Article
    Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928
    Insert
    10 amino acid glycine/serine linker + 5alanines
    Use
    Synthetic Biology
    Tags
    FLAG and zif268
    Expression
    Bacterial
    Promoter
    UV5
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    128165-plasmid-map-sequence-id-267573

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  49. MSP7-bio

    Plasmid
    #47735
    Purpose
    Expresses enzymatically monobiotinylated full-length MSP7 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised MSP7
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47735-plasmid-map-sequence-id-68747

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  50. pLJSRSF7-T7-6xHis-SUMO-MRT-CBD

    Plasmid
    #253350
    Purpose
    For the expression of group II intron encoded reverse transcriptase MarathonRT with N-terminal SUMO-tag and C-terminal CBD-tag in E. coli.
    Depositor
    Peter Sarin
    Article
    Pedor et al Bio-protocol 2026, 16(11): e5703. DOI: 1
    Insert
    6xHis-SUMO-MarathonRT with added C-terminal CBD-tag originating from the backbone.
    Tags
    6xHis-SUMO and CBD
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    253350-plasmid-map-sequence-id-507308

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Showing: 1901 - 1950 of 4645 results