We narrowed to 79,968 results for: Rest

Showing: 1901 - 1950 of 79968 results

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pTatAkBxCs

Plasmid

#178544

Purpose

pTatABC with KpnI, XbaI, and KpnI restriction sites after TatA, TatB, and TatC, respectively.

Depositor

Siegfried Musser

Article

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

TatA, TatB, TatC

Expression

Bacterial

Promoter

araC

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST-Spe3-RfA

Plasmid

#186370

Purpose

Destination vector for the expression of a protein of interest into embryos, by mRNA microinjection

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSM2

Plasmid

#85753

Purpose

pDONR split marker vector (GenBank accession number: KX904529) for fungal gene deletion. Used with pSM1.

Depositor

Damien Fleetwood

Article

Rahnama et al J Microbiol Methods. 2016 Dec 22. pii: S

Type

Empty backbone

Expression

Bacterial

Promoter

N/A

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pSM1

Plasmid

#85752

Purpose

pDONR split marker vector (GenBank accession number: KX904528) for fungal gene deletion. Used with pSM2.

Depositor

Damien Fleetwood

Article

Rahnama et al J Microbiol Methods. 2016 Dec 22. pii: S

Type

Empty backbone

Expression

Bacterial

Promoter

N/A

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) TRIM30C

Plasmid

#87083

Purpose

Murine homologues of human Trim5

Depositor

Jeremy Luban

Article

Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

Insert

TRIM30C (Trim30c Mouse)

Expression

Mammalian

Available Since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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pRL092 pattB_cryaa:Venus_MCS

Plasmid

#213513

Purpose

attB-containing vector backbone, for use in restriction enzyme- or PCR-based cloning

Depositor

Christian Mosimann

Article

Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

Type

Empty backbone

Use

Zebrafish expression

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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LXRE_Luc

Plasmid

#177622

Purpose

synthetic 3X LXRE promoter linked to luciferase in the pTAL vector

Depositor

Thomas Burris

Article

Griffett et al ACS Chem Biol. 2022 Apr 13. doi: 10.1021

Insert

3X LXRE

Use

Luciferase

Expression

Mammalian

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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HLA_A2402

Plasmid

#165010

Purpose

Expression of HLA-A*24:02 cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

HLA-A*24:02 (HLA-A Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-Tornado-nLuc

Plasmid

#212611

Purpose

For expression of a circular nLuc mRNA. To express your gene of interest clone into the BsiWI, SacII sites and add the GCCATGTcTATGTGG 5' of the SacII site.

Depositor

Samie Jaffrey

Article

Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

Insert

Tornado-CVB3-nLuc

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCI-NLS-BirA-2A-mCherry

Plasmid

#127781

Purpose

BirA construct in a PCI nls backbone driving mCherry activity

Depositor

Tatjana Sauka-Spengler

Article

Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

Insert

BirA

Use

Unspecified

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pHIV-EGFP

Plasmid

#21373

Purpose

self-inactivating lentiviral plasmid for co-expression of your gene of interest and EGFP

Depositor

Bryan Welm, Zena Werb

Article

Welm et al Cell Stem Cell. 2008 Jan 10. 2(1):90-102

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

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pSIP403

Plasmid

#122028

Purpose

Plasmid for expression of gene of interest in Lactobacillus plantarum and L. sakei

Depositor

Lars Axelsson, Geir Mathiesen

Article

Sorvig et al Microbiology. 2005 Jul;151(Pt 7):2439-49

Insert

gusA

Expression

Bacterial

Promoter

sppA

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pRVL-4

Plasmid

#104582

Purpose

Contains human kappa CL domain, for cloning of antibody VL domains using SapI restriction enzyme.

Depositor

Daniel Christ

Article

Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

Insert

Human CL kappa

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pDawn

Plasmid

#43796

Purpose

Empty backbone for blue-light-regulated expression of a gene of interest. Expression is induced under blue illumination.

Depositor

Andreas Moeglich

Article

Ohlendorf et al J Mol Biol. 2012 Mar 2;416(4):534-42. do

Type

Empty backbone

Expression

Bacterial

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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HLA_A0101

Plasmid

#165009

Purpose

Expression of HLA-A*01:01 cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

HLA-A*01:01 (HLA-A Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pREDawn-AmpR-MCS

Plasmid

#188978

Purpose

For red light-induced bacterial expression of gene of interest (AmpR)

Depositor

Heikki Takala

Article

Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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MscL(G22S)-c-term-cpGFP-ERexp

Plasmid

#195346

Purpose

Control plasmid for the MscL tension biosensor

Depositor

Allen Liu

Article

Hsu et al ACS Sens. 2023 Jan 27;8(1):12-18. doi: 1

Insert

MscL(G22S)-c-term-cpGFP-ERexp

Use

Lentiviral

Expression

Mammalian

Promoter

CMVd2

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRL093 pattB_exorh:EGFP_MCS

Plasmid

#213514

Purpose

attB-containing vector backbone, for use in restriction enzyme- or PCR-based cloning

Depositor

Christian Mosimann

Article

Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

Type

Empty backbone

Use

Zebrafish expression

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pDusk

Plasmid

#43795

Purpose

Empty backbone for blue-light-regulated expression of a gene of interest. Expression is repressed under blue illumination.

Depositor

Andreas Moeglich

Article

Ohlendorf et al J Mol Biol. 2012 Mar 2;416(4):534-42. do

Type

Empty backbone

Expression

Bacterial

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pRVL-1

Plasmid

#104579

Purpose

Contains mouse kappa CL domain, for cloning of antibody VL domains using SapI restriction enzyme.

Depositor

Daniel Christ

Article

Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

Insert

Mouse CL kappa

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-mCherry-CDK4KTR

Plasmid

#126680

Purpose

Fluorescent reporter for CDK4/6 activity

Depositor

Hee Won Yang

Article

Yang et al Elife. 2020 Apr 7;9. pii: 44571. doi: 10

Insert

RB cterm

Use

Lentiviral

Expression

Mammalian

Available Since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

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HyperNova/pRSETB

Plasmid

#221456

Purpose

Fast-maturating photosensitizing fluorescent protein for chromophore-assisted light inactivation

Depositor

Kiwamu Takemoto

Article

Shidara et al Commun Biol. 2024 Aug 6;7(1):945. doi: 1

Insert

HyperNova

Expression

Bacterial

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-OmpT

Plasmid

#68862

Purpose

construct for expression of OmpT protease in the pET28a plasmid using the restriction sites NcoI and EcoRI.

Depositor

Neil Kelleher

Article

Wu et al Nat Methods. 2012 Jun 17;9(8):822-4. doi

Insert

OmpT (ompT )

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-Lyn11-FRB

Plasmid

#155228

Purpose

membrane anchor for FKBP-FRB system

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

Membrane targeted (through Lyn11) FRB for use with FKBP-FRB dimerization system

Expression

Mammalian

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pMIAII-Cd4

Plasmid

#194401

Purpose

Retroviral expression of mouse CD4

Depositor

Michael Carroll

Article

Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

Insert

Cd4 (Cd4 Mouse)

Use

Retroviral

Mutation

WT

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pREDawn-StrR-MCS

Plasmid

#188979

Purpose

For red light-induced bacterial expression of gene of interest (StrR)

Depositor

Heikki Takala

Article

Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUS_B2

Plasmid

#31019

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

LacZ + BsaI restriction sites

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pSL1-GFP

Plasmid

#180420

Purpose

Kan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes a GFP promoter.

Depositor

Nancy Moran

Article

Li et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

GFP

Expression

Bacterial

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pSFVCs-lacZ

Plasmid

#92076

Purpose

This is a high level expression plasmid for production of recombinant genomic SFV RNA encoding a C-beta galactosidase fusion protein.

Depositor

Henrik Garoff

Article

Sjoberg et al Biotechnology (N Y). 1994 Nov;12(11):112

Insert

C-lacZ

Use

C-lacz expression plasmid, for run-off transcript…

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pACλCI-β-flap (831-1057)

Plasmid

#53733

Purpose

Expression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.

Depositor

Ann Hochschild

Article

Dove et al Nature. 1997 Apr 10;386(6625):627-30.

Insert

λCI-β-flap fusion

Expression

Bacterial

Promoter

lac UV5

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-CaM-V5-sTb(N)

Plasmid

#219783

Purpose

sTb(N) half of CaST for neuron expression

Depositor

Christina Kim

Article

Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

Insert

CaM-V5-sTb(N)

Use

AAV

Tags

V5

Expression

Mammalian

Promoter

Synapsin

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pSkylan-NS-N1

Plasmid

#86785

Purpose

for fusion of gene of interest with a monomeric reversibly photoswitchable fluorescent protein, Skylan-NS

Depositor

Pingyong Xu

Article

Zhang et al Proc Natl Acad Sci U S A. 2016 Sep 13;11

Type

Empty backbone

Tags

Skylan-NS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pQFT-mNGsacB

Plasmid

#203676

Purpose

pQFT with mNeonGreen and sacB under control of PQJ

Depositor

Urs Jenal

Article

Klotz et al Appl Environ Microbiol. 2023 May 18:e002

Inserts

mNeonGreen
sacB

Expression

Bacterial

Mutation

Has synonymous (silent) mutations to remove BsrGI…

Promoter

PQJ

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR-v2 sgBAK1

Plasmid

#211520

Purpose

Deletes BAK1

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgBAK1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pREDusk-AmpR-MCS

Plasmid

#188974

Purpose

For red light-repressed bacterial expression of gene of interest (AmpR)

Depositor

Heikki Takala

Article

Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBIS-GALkFLP(URA)

Plasmid

#49460

Purpose

overexpression of Flp step-arrest mutant in yeast, URA selection

Depositor

Marc Gartenberg

Article

Tsalik et al Yeast. 1998 Jun 30;14(9):847-52.

Insert

Flp(H305L) (flp Budding Yeast)

Expression

Yeast

Mutation

H305L, ‘step-arrest’ mutant,

Promoter

GAL10

Available Since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
HLA_B5101

Plasmid

#165011

Purpose

Expression of HLA-B*51:01 cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

HLA-B*51:01 (HLA-B Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR-v2 sgCASP8

Plasmid

#211519

Purpose

Deletes CASP8

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgCASP8

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
C1-iRFP-iLid-CaaX

Plasmid

#155229

Purpose

membrane marker

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

iRFP C-term tagged with the ilID motif and the CaaX sequence derived from Kras4b. Membrane control

Tags

iRFP-iLid

Expression

Mammalian

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST-Spe3-RfA-Venus

Plasmid

#186372

Purpose

Destination vector for the expression of a fusion of an N-ter protein of interest with Venus into embryos, by mRNA microinjection

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFN18A-HaloTag-SpyCatcher

Plasmid

#206041

Purpose

HaloTag-Spy0128-(Protein of Interest)-Spy0128-SpyCatcher-8xHisTag

Depositor

Sergi Garcia-Manyes

Article

Tapia-Rojo et al Nat Protoc. 2024 Mar 11. doi: 10.1038/s4

Type

Empty backbone

Tags

HaloTag and SpyCatcher-8His

Expression

Bacterial

Promoter

T7

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pD441_WT_hi

Plasmid

#128857

Purpose

High copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
isce rfp/cfp

Plasmid

#83323

Purpose

fluorescent Isce joint substrate

Depositor

Katheryn Meek

Article

Neal et al J Immunol. 2016 Apr 1;196(7):3032-42. do

Insert

RFP +CFP joining substrate

Use

Synthetic Biology

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR-v2 sgATG7

Plasmid

#211534

Purpose

Deletes ATG7

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgATG7

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJ211_rha_WT_low

Plasmid

#128851

Purpose

Low copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pREDusk-StrR-MCS

Plasmid

#188972

Purpose

For red light-repressed bacterial expression of gene of interest (StrR)

Depositor

Heikki Takala

Article

Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUS_B4

Plasmid

#31021

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

LacZ + BsaI restriction sites

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CMV-CaM-V5-sTb(N)

Plasmid

#219779

Purpose

sTb(N) half of CaST for HEK cell expression

Depositor

Christina Kim

Article

Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

Insert

CaM-V5-sTb(N)

Use

AAV

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
290 RFP/CFP

Plasmid

#83322

Purpose

fluorescent coding joint substrate

Depositor

Katheryn Meek

Article

Neal et al J Immunol. 2016 Apr 1;196(7):3032-42. do

Insert

RFP +CFP joining substrate

Use

Synthetic Biology

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pJMC-L1-Donor-Esp3I

Plasmid

#197842

Purpose

Donor vector to restore L1 destination via Esp3I

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

None

Use

Unspecified

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 79,968 results for: Rest