We narrowed to 16,142 results for: GRN

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pVC-Ds-DrU6a:sgRNA-Ds

Plasmid

#119069

Purpose

To clone sgRNA spacer sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:sgRNA

Expression

Bacterial

Promoter

Zebrafish U6a promoter

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-malG

Plasmid

#89951

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting malG.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

malG gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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px330BRG1 gRNA

Plasmid

#165585

Purpose

Insertion of BRG1 degron

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

BRG1 (Smarca4 Mouse)

Expression

Mammalian

Mutation

NA

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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EPHB4-gRNA1/2

Plasmid

#139974

Purpose

gRNA used for knockin EGFP into EPHB4 allele

Depositor

James Thomson

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jul 25;11

Insert

EPHB4 gRNA

Expression

Mammalian

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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EFNB2-gRNA3/4

Plasmid

#139973

Purpose

gRNA used for knockin tdTomado into EFNB2 allele

Depositor

James Thomson

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jul 25;11

Insert

EFNB2-gRNA

Expression

Mammalian

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA EMX1

Plasmid

#128121

Purpose

Co-expresses sgRNA EMX1, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

sgRNA EMX1 + GFP

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

RSV/U6

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pHU6_Ch13_102010574-gRNA-for

Plasmid

#81218

Purpose

pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 3' region of pCALNL_Ch13 reporter

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pKFSS1_Psyn-sgRNA500

Plasmid

#149557

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Psyn and pSyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-sgRNA_Drosha_4

Plasmid

#79863

Purpose

CRISPR/Cas9 DROSHA

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

sgRNA Drosha

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
pMST665_BB3_L 23_gRNAempty_cas9_BbsI

Plasmid

#90278

Purpose

BB3_L 23_gRNAempty_cas9_BbsI; CRISPR plasmid with empty gRNA spacer to incorporate gRNA, Cas9

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

Cas9

Use

A. niger

Expression

Bacterial

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-sgRNA_Drosha_1

Plasmid

#79860

Purpose

CRISPR/Cas9 DROSHA

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

sgRNA Drosha

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
pX330-sgRNA_Drosha_2

Plasmid

#79861

Purpose

CRISPR/Cas9 DROSHA

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

sgRNA Drosha

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
pX330-sgRNA_Drosha_3

Plasmid

#79862

Purpose

CRISPR/Cas9 DROSHA

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

sgRNA Drosha

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
pKDsgRNA-hisG

Plasmid

#89954

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting hisG.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

hisG gRNA

Expression

Bacterial

Promoter

pTet

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-AasgRNA3.8.3

Plasmid

#121959

Purpose

Mammalian expression, Transcription regulation, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b single chimeric gRNA, MS2 hairpin inserted

Expression

Mammalian

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-sgRNA_Dicer_1

Plasmid

#68807

Purpose

specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.

Depositor

Constance Ciaudo

Article

Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

Insert

sgRNA mouse Dicer1

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pHU6_gRNA_Ch10-rev2

Plasmid

#81207

Purpose

for targeting Ch10 psuedo gix site in PCDH15 gene (five base pairs from the cas9 site and 5' of gix site)

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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MYC sgRNA4

Plasmid

#100556

Purpose

Expresses MYC sgRNA4. Target sequence: GTAATTCCAGCGAGAGGCAG

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

MYC sgRNA4

Promoter

U6

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pEX-A-U6-MiSgRNA_PuroR

Plasmid

#84781

Purpose

Expresses minor satellite-specific sgRNA.

Depositor

Heinrich Leonhardt

Article

Schmidtmann et al Nucleus. 2016 Sep 27:0.

Insert

minor satellite-specific sgRNA

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

Nov. 6, 2019

Availability

Academic Institutions and Nonprofits only

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p426_Cas9_gRNA-ARS720a

Plasmid

#87392

Purpose

All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS720a sequence CAACAATTGTTACAATAGTA in yeast chromosome 7.

Depositor

Aindrila Mukhopadhyay

Article

Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

Insert

Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS720a

Use

CRISPR

Promoter

ADH1, pTyrosine

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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