We narrowed to 14,742 results for: RING

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pLenti-hSynapsin-Cre-WPRE

Plasmid

#86641

Purpose

Expresses Cre in mammalian cells

Depositor

Fan Wang

Article

Sakurai et al Neuron. 2016 Nov 23;92(4):739-753. doi:

Insert

Cre

Use

Cre/Lox, Lentiviral, Mouse Targeting, and Synthet…

Expression

Mammalian

Promoter

hSynapsin

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pFC334

Plasmid

#87846

Purpose

AMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymes

Depositor

Uffe Mortensen

Article

Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

Inserts

Cas9
argB
yA specific sgRNA

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Promoter

A. nidulans gpdA promoter with Hammerhead ribozym…

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pCAT005

Plasmid

#171632

Purpose

2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting B2M and an EF1-a promoter expressing mNeonGreen

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-B2M
mNeon

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1-a and U6

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2

Plasmid

#163403

Purpose

Expresses BMPR-2 in mammalican cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Expression

Mammalian

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-WHaloCaMP1b-EGFP

Plasmid

#205305

Purpose

Expression of WHaloCaMP1b-EGFP fusion in E. coli

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1b-EGFP

Expression

Bacterial

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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L0-I35 p35S-XVE-pEstradiol GG0-GG1

Plasmid

#195904

Purpose

Level 0 inducibile estradiol promter and XVE activator protein (p35S-XVE gene-tRBCS-tNOS-LexA-p35S with GG0 and GG1 golden gate linkers)

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

p35S-XVE-LexA-p35S

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pFC333

Plasmid

#87844

Purpose

AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker

Depositor

Uffe Mortensen

Article

Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

Inserts

Cas9
ble (bleomycin resistance marker)

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Promoter

Aspergillus nidulans tef1 promoter and Aspergillu…

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pAG654 pFAST

Plasmid

#172861

Purpose

Expresses pFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

pFAST

Tags

Myc tag (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pGPA1

Plasmid

#115476

Purpose

Generation of mariner-based transposon mutant libraries in Enterococcus faecium

Depositor

Willem van Schaik

Article

Zhang et al BMC Genomics. 2017 Nov 21;18(1):893. doi

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCI-SEP GluR2(R)

Plasmid

#24001

Depositor

Robert Malinow

Article

Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

Insert

GluR2 (Gria2 Rat)

Tags

GluR1 signal peptide (aa1-22) and SEP (supereclip…

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV.CAGFLEX.(cyto).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209657

Purpose

Expression of iATPSnFR2, low affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.HaloTag

Use

AAV

Mutation

A95A.A119L

Promoter

CAG

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCLucIPZ

Plasmid

#53222

Purpose

Constitutive expression of Cypridina luciferase, puromycin N-acetyl-transferase and shRNAmir of interest

Depositor

Thorsten Stiewe

Article

Charles et al Nat Commun. 2014 Jun 3;5:3981. doi: 10.1

Inserts

Cypridina luciferase
non-silencing shRNA

Use

Lentiviral

Expression

Mammalian

Available Since

June 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pQE-80L iLID (C530M)

Plasmid

#60408

Purpose

E. coli expression of iLID (C530M)

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

iLID C530M

Tags

His

Expression

Bacterial

Mutation

C530M

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
pLL7.0: Venus-iLID-Mito (From ActA)

Plasmid

#60413

Purpose

Mammalian Expression of Venus-iLID-Mito

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

Venus-iLID-Mito

Use

Cre/Lox and Lentiviral

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-Cdc42-2G

Plasmid

#68813

Purpose

FRET-based biosensor reporting on endogenous Cdc42 activation (for Lentivirus production)

Depositor

Olivier Pertz

Article

Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

Insert

Cdc42 activation reporter

Use

Lentiviral

Tags

His and Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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SlugCas9-HF

Plasmid

#163796

Purpose

Expresses highly specific SlugCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Mutation

SlugCas9 (R247A, N415A, T421A, R656A)

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET28b_RanWT

Plasmid

#186270

Purpose

Expression vector of His-RanQ69L in E.coli

Depositor

Masahito Ikawa

Article

Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

GTP-binding nuclear protein Ran (Ran Mouse)

Tags

His tagged

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iATPSnFR2.S29W.A95K.HaloTag

Plasmid

#209651

Purpose

Expression of iATPSnFR2, high affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.S29W.HaloTag

Use

AAV

Mutation

S29W.A95K

Promoter

CAG

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB) (PX460)

Plasmid

#48873

Purpose

Cas9n (D10A nickase mutant) from S. pyogenes

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Has Service

Cloning Grade DNA

Insert

hSpCas9n

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Mutation

Cas9 D10A nickase mutant

Promoter

Cbh

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

Plasmid

#42229

Purpose

This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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