164,577 results

1. LVXN-Neo-NSD2-E1099K

   Plasmid

   #86011

   Purpose

   express NSD2 E1099K mutant in mammalian cells

   Depositor

   Darrin Stuart

   Article

   Jaffe et al Nat Genet. 2013 Nov;45(11):1386-91. doi:

   Insert

   NSD2 E1099K mutant (NSD2 Human)
   

   Use

   Tags

   Flag tag  D Y K D D D D K

   Expression

   Mammalian

   Mutation

   E1099K; K1002R (please see depositor comments bel…

   Promoter

   CMV

   Available Since

   Jan. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

2. Sniper2L

   Plasmid

   #193856

   Purpose

   Expresses human codon-optimized Sniper2L and blasticidin resistance: EFS promoter-Sniper2L-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Chem Biol. 2023 Mar 9. doi: 10.1038/

   Insert

   Sniper2L
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   E1007L

   Promoter

   EFS

   Available Since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pL-RPB1

   Plasmid

   #10981

   Depositor

   Jeff Strathern

   Article

   Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

   Insert

   RPB1 (RPO21 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Wild type RPB1 gene

   Promoter

   Available Since

   Dec. 16, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDual_dsCas9_Blast

   Plasmid

   #214681

   Purpose

   Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two empty sgRNA cloning sites

   Depositor

   Kevin Janes

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHTN-HaloTag_BCL11A:1-773

   Plasmid

   #211014

   Purpose

   Mammalian expression of full-length human BCL11A. N-terminal HaloTag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   BCL11A:M1-F773 (BCL11A Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   isoform 2

   Promoter

   CMV

   Available Since

   Feb. 21, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pDEST-cDNA5-FRT/TO-3*Flag-APEX2 N-term

   Plasmid

   #182925

   Purpose

   Express FLAG epitope and APEX2-tagged fusion protein in mammalian cells.

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Type

   Empty backbone

   Use

   Tags

   APEX2 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMP11

   Plasmid

   #215559

   Purpose

   pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin

   Depositor

   Brian Pfleger

   Article

   Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

   Inserts

   Cas9
   gRNA targeting pBR322 origin of replication
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23105 and tet

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFEB promoter-luciferase reporter

   Plasmid

   #66801

   Purpose

   Reporter for activity of TFEB gene promoter

   Depositor

   Albert La Spada

   Article

   Tsunemi et al Sci Transl Med. 2012 Jul 11;4(142):142ra

   Insert

   TFEB promoter (Tfeb Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. PKC alpha CAT

   Plasmid

   #21234

   Depositor

   Bernard Weinstein

   Article

   Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

   Insert

   PKC alpha (PRKCA Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta 1-325

   Promoter

   Available Since

   Aug. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-PBZ-mRuby2

    Plasmid

    #110650

    Purpose

    expresses a PAR-binding (PBZ) domain fused to mRuby2

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

    Insert

    PBZ domain from APLF, amino acids 371-451 (APLF Human)
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-FGFR3

    Plasmid

    #116742

    Purpose

    Lentiviral expression of FGFR3

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    FGFR3 (FGFR3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMV-OMM-GCaMP6f

    Plasmid

    #127874

    Purpose

    Fluorescent reporter for mitochondrial outer membrane Ca2+

    Depositor

    Timothy Ryan

    Article

    Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

    Insert

    Outer membrane localized GCaMP6f
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP NFAT5 (no EGFP)

    Plasmid

    #13627

    Depositor

    Anjana Rao

    Article

    Lopez-Rodriguez et al Proc Natl Acad Sci U S A. 1999 Jun 22. 9

    Insert

    NFAT5 (NFAT5 Human)
    

    Use

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEF6-CSPG4-myc-his

    Plasmid

    #69037

    Purpose

    Expression of CSPG4 in mammalian cells

    Depositor

    Wensheng Wei

    Article

    Yuan et al Cell Res. 2015 Feb;25(2):157-68. doi: 10

    Insert

    CSPG4 (CSPG4 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1 alpha

    Available Since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMycVec2

    Plasmid

    #32361

    Depositor

    Michael Niederweis

    Article

    Kaps et al Gene. 2001 Oct 31;278(1-2):115-24.

    Insert

    pJAZ38 ori
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCPP5372

    Plasmid

    #128733

    Purpose

    Gateway destination low-copy number plasmid for expression of any effector from avrPto promoter with C-terminal HA tag

    Depositor

    Alan Collmer

    Article

    Oh et al J Bacteriol. 2007 Nov;189(22):8277-89. E

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRDB_347

    Plasmid

    #231114

    Purpose

    CBE activity-based selection cloning vector

    Depositor

    David Root, John Doench

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CALM-pmCherryN1

    Plasmid

    #27691

    Depositor

    Christien Merrifield

    Article

    Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

    Insert

    CALM (Picalm Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX601-GFP

    Plasmid

    #84040

    Purpose

    Staphylococcus aureus (SaCas9) conjugated with GFP

    Depositor

    Yuet Wai Kan

    Article

    Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

    Insert

    SaCas9
    

    Use

    AAV and CRISPR

    Tags

    NLS and T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. GALT-APEX2

    Plasmid

    #215554

    Purpose

    Stable, inducible expression in Golgi

    Depositor

    Nevan Krogan

    Article

    Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

    Insert

    APEX2-NES-GalT-EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. TFORF1167

    Plasmid

    #142744

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    EBF3 (EBF3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFUS-WT-VC

    Plasmid

    #175068

    Purpose

    expresses FUS wildtype tagged to mVenus and mCherry in mammalian cells

    Depositor

    Arnold Boersma

    Article

    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

    Insert

    FUS glycine rich protein (FUS Human)
    

    Use

    Tags

    HA and mVenus + mCherry

    Expression

    Mammalian

    Mutation

    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

    Promoter

    CMV Promoter

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-EF1a-mCherry-IRES-Dre

    Plasmid

    #55633

    Purpose

    Expresses Dre in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Inserts

    mCherry
    Dre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a and Ef1a/IRES

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Nrp2.1-Fc-His

    Plasmid

    #72098

    Purpose

    Expresses the extracellular region of the Neuropilin 2, isoform 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Nrp2.1 (Nrp2 Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSIN4-EF1a-GATA1-IRES-Puro

    Plasmid

    #61062

    Purpose

    lentiviral vector for GATA1

    Depositor

    Igor Slukvin

    Article

    Elcheva et al Nat Commun. 2014 Jul 14;5:4372. doi: 10.

    Insert

    GATA1 (GATA1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    EF1alpha

    Available Since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGIPz-mPD-L1 3SA

    Plasmid

    #121489

    Purpose

    Expression of mouse PD-L1 3SA

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    PD-L1 3SA
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S175A, T179A, S183A

    Promoter

    CMV

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. SDC4A-c001

    Plasmid

    #205119

    Purpose

    SDC4 protein expression

    Depositor

    Paul Brennan

    Insert

    SDC4 (SDC4 Human)
    

    Use

    Tags

    AviTag-6His and PTPRSsp

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. 1436 pcDNA3 Flag HA

    Plasmid

    #10792

    Purpose

    pcDNA3 vector with added Flag, HA tags

    Depositor

    William Sellers

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pF142 pAcGFP1 SOD1 A4V

    Plasmid

    #26403

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    A4V

    Promoter

    Available Since

    Nov. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3-HA-Sumo1

    Plasmid

    #21154

    Purpose

    Mammalian expression of human Sumo1 with HA tag

    Depositor

    Junying Yuan

    Article

    Terui et al J Biol Chem. 2004 Jul 2. 279(27):28257-6

    Insert

    pcDNA3-HA-Sumo1 (SUMO1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge