We narrowed to 41,619 results for: Eras

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pAVA-Gal11p-LGF2

Plasmid

#127480

Purpose

Positive control for the AVA-seq system. Gal11p (lambda CI associated) and LGF2 (RNAp associated) interaction

Depositor

Joel Malek

Article

Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

Insert

Gal11p-LGF2 (GAL11 Budding Yeast)

Expression

Bacterial

Available Since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3/HIP1/ΔLD

Plasmid

#31253

Depositor

Theodora Ross

Article

Rao et al J Clin Invest. 2002 Aug . 110(3):351-60.

Insert

Huntingtin-interacting protein 1 (HIP1 Human)

Expression

Bacterial

Mutation

Deltion of nucleotides 767-1335

Available Since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3/HIP1/ΔC

Plasmid

#31250

Depositor

Theodora Ross

Article

Rao et al J Clin Invest. 2002 Aug . 110(3):351-60.

Insert

Huntingtin-interacting protein 1 (HIP1 Human)

Expression

Bacterial

Mutation

Deltion of nucleotides 1336-2513 1386bp C to T…

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
MK13_HUMAN_D0

Plasmid

#79693

Purpose

This plasmid encodes the kinase domain of MK13. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MK13

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pNHT7::RPL15::hGluc

Plasmid

#186768

Purpose

An mRNA production vector encoding human codon-optimized gaussia luciferase (hGluc) with RPL15 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Gaussia luciferase

Promoter

T7

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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LB334 (Bcl-2 from -1287 to -1640)

Plasmid

#15241

Depositor

Linda Boxer

Article

Wilson et al Mol Cell Biol. 1996 Oct . 16(10):5546-56

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Mutation

Bcl-2 fragment from -1287 to -1640 with P1

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
O-MARS-11

Plasmid

#166541

Purpose

scFv of a human scaffold targeting Methionyl-tRNA synthetase. Antigen coverage aa 1-225 of 900

Depositor

Susanne Gräslund

Article

Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

Insert

single chain-Fv, scFv

Use

Recombinant antibody expression

Tags

3xFLAG, His6 and OmpA leader sequence

Expression

Bacterial

Promoter

LacZ

Available Since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLuc-CDS-m215-2

Plasmid

#42102

Depositor

Kumiko Ui-Tei

Article

Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

Insert

ZEB1 (ZEB1 Human)

Use

Luciferase

Tags

Renilla luciferase

Expression

Mammalian

Mutation

Silent mutations were introduced in the seed-comp…

Promoter

SV40

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pv6_dPC_Chromo_TAF3_Phd

Plasmid

#179403

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of dPC_Chromo - TAF3_Phd

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

dPC_Chromo - TAF3_Phd

Tags

AviTag and EGFP

Expression

Mammalian

Promoter

CAGGS

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pPbuCas13b-gRNA-NT

Plasmid

#184568

Purpose

Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PbuCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

Constitutive expression of single-spacer CRISPR array witha nontargeting spacer for PbuCas13b in bacteria.

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pGL421_MLL_promoter

Plasmid

#118695

Purpose

Express MLL/KMT2A promoter for luciferase assay

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Insert

MLL_promoter

Use

Luciferase

Promoter

MLL/KMT2A promoter

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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PAK7_HUMAN_D0

Plasmid

#79706

Purpose

This plasmid encodes the kinase domain of PAK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

PAK7 (PAK5 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pQCascade-IS3

Plasmid

#140626

Purpose

Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS3. The crRNA-IS3 targets IS3 loci in Escherchia coli.

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS3

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pBEG R2-iPuro-L3 #BGV205

Plasmid

#48987

Purpose

Supplies the selection marker (puromycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

Depositor

David Dankort

Article

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Insert

puromycin resistance

Use

Gateway cloning vector

Tags

IRES (weak)

Promoter

NA

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pGL3-24p3/LCN2-282kB mut-LUC

Plasmid

#46867

Purpose

murine 24p3 minimal promoter with NF-kB mutation as a luciferase reporter

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

24p3/lipocalin 2 proximal promoter

Use

Luciferase

Mutation

NF- kB site is mutated (see comments)

Promoter

24p3 promoter (inactive)

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only
pLV Reep4-V5

Plasmid

#175120

Purpose

Lentiviral expression of mouse Reep4-V5

Depositor

Larry Gerace

Article

Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

Insert

Reep4 (Reep4 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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VRK3_HUMAN_D0

Plasmid

#79681

Purpose

This plasmid encodes the kinase domain of VRK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

VRK3 (VRK3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pNHT7::RPL15::sNluc2

Plasmid

#186763

Purpose

An improved mRNA product vector encoding a codon-optimized nanoluciferase (sNluc2) with RPL15 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Promoter

T7

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-24p3/LCN2-282C/EBP mut-LUC

Plasmid

#46868

Purpose

murine 24p3 minimal promoter with C/EBP mutation k as a luciferase reporter

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

24p3/lipocalin 2 proximal promoter

Use

Luciferase

Mutation

CEBP site is mutated (see comments)

Promoter

24p3 promoter (inactive)

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only
pBS/U6/RNAi SadBs shRNA mouse

Plasmid

#67152

Purpose

pBS/U6 containing shRNA to mouse SADBs

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

BRSK1 (Brsk1 Mouse)

Expression

Mammalian

Promoter

U6

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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