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pAS162

Plasmid

#194485

Purpose

synGR Cassette

Depositor

Ahmad Khalil

Publication

Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

Insert

synGR Cassette

Use

Synthetic Biology

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pWS5396-CRISPRai-KanR

Plasmid

#182835

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - KanR marker

Depositor

Rodrigo Ledesma-Amaro

Publication

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pJ2072.2_c1

Plasmid

#140675

Purpose

CRISPRlator control (“open ring”)

Depositor

Yolanda Schaerli

Publication

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

CRISPRlator control (“open ring”)

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAW209

Plasmid

#113188

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array

Depositor

Jennifer Doudna

Publication

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pUWL201PW-creDE

Plasmid

#112241

Purpose

Streptomyces/E.coli shuttle expression vector encoding creD and creE

Depositor

Emily Balskus

Publication

Waldman et al J Org Chem. 2018 May 29. doi: 10.1021/ac

Inserts

creD
creE

Expression

Bacterial

Available since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pTK105

Plasmid

#59562

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn EYFP-PEST

Depositor

Ron Weiss

Publication

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) 2xK-turn EYFP-PEST

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK034

Plasmid

#65141

Purpose

Encodes mRuby2 as a Type 3 part to be used in the Dueber YTK system

Depositor

John Dueber

Publication

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

mRuby2

Expression

Bacterial

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAC025

Plasmid

#227597

Purpose

Lentiviral backbone used when cloning pooled prime editing guide RNA libraries already containing a tevopreQ1 RNA motif

Depositor

Brittany Adamson

Publication

Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pEBFP2

Plasmid

#165831

Purpose

EBFP2blue fluorescent protein CDS

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

EBFP2

Expression

Insect

Available since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pTK

Plasmid

#165841

Purpose

ganciclovir sensitivity marker

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

SR39TK

Expression

Insect

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pQP-2473

Plasmid

#138976

Purpose

Nucleus-to-cytoplasm relocalization of genomically expressed GFP-fusion using NIR light controlled intrabody.

Depositor

Vladislav Verkhusha

Publication

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-BphP1-NES-T2A- iB(GFP)-NES-mCherry-QPAS1- NLS-NotI

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCFD3-MS2tail-SAM

Plasmid

#78905

Purpose

Vector for expressing a single sgRNA with SAM MS2 tail, under U6:3 promoter

Depositor

Norbert Perrimon

Publication

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available since

Aug. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pPROBE-TT'

Plasmid

#37823

Depositor

Steven Lindow

Publication

Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

Promotorless gfp reporter gene

Available since

July 23, 2012

Availability

Academic Institutions and Nonprofits only
PJEBAN3

Plasmid

#203682

Purpose

Expresses Yellow Fluorescent Protein (YFP) in Listeria monocytogenes

Depositor

Tine Rask Licht

Publication

Andersen et al BMC Microbiol. 2006 Oct 3;6:86. doi: 10.

Insert

Yellow Fluorescent Protein (YFP)

Expression

Bacterial

Available since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pUREF-EX

Plasmid

#199331

Purpose

A pEF-BOS-EX-derived expression vector that contains an SV40 promoter-driven puromycin resistance gene

Depositor

Rikiro Fukunaga

Publication

Fujii et al Genes Cells. 2023 Mar 2. doi: 10.1111/gt

Type

Empty backbone

Expression

Mammalian

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Aac.4

Plasmid

#158406

Purpose

Express single gRNA with Aac.4 scaffold (with four MS2 binding sites) under ZmUbi promoter

Depositor

Yiping Qi

Publication

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Maize ubiquitin 1

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mTagBFP2-T2A-dre

Plasmid

#124832

Purpose

Monomeric TagBFP2 and dre recombinase

Depositor

Mario Capecchi

Insert

mTagBFP2-T2A-dre

Expression

Mammalian

Promoter

CAG

Available since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

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pSrpR-S2

Plasmid

#74692

Purpose

SrpR-S2 gate

Depositor

Christopher Voigt

Publication

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSS2-SrpR-PSrpR-YFP

Expression

Bacterial

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pBM3R1-B2

Plasmid

#74679

Purpose

BM3R1-B2 gate

Depositor

Christopher Voigt

Publication

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSB2-BM3R1-PBM3R1-YFP

Expression

Bacterial

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pBM3R1-B1

Plasmid

#74678

Purpose

BM3R1-B1 gate

Depositor

Christopher Voigt

Publication

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSB1-BM3R1-PBM3R1-YFP

Expression

Bacterial

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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