We narrowed to 18,247 results for: multi
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Plasmid#65702PurposeExpresses YNL-H2B in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthistone H2B
UseLuciferaseTagsYNLExpressionMammalianPromoterCMVAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T7-6h-GST-α-Synuclein
Plasmid#225224PurposeAlpha synuclein gene fused with gst gene under t7 promoter for bacterial expression of alpha synuclein protein.DepositorTags6xHis Tag and TEV Cleavage SiteExpressionBacterialPromoterT7Available sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOPINB-M1-di-Ub(G76V)-HOIL-1
Plasmid#229539PurposeConstitutively active M1-di-ubiquitin HOIL-1 fusion protein for expression in E. coliDepositorTags6xHis-3CExpressionBacterialMutationChanged Gly 76 to Val and changed Gly 76 to ValPromoterT7Available sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_AR-RE_MLPmin_BC1011-luc2
Plasmid#227106PurposeAndrogen receptor response element for luciferase and barcode assays; barcode BC1011DepositorInsert8x clustered AR response element linked to MLP minimal promoter driving barcode 1011 and a luciferase reporter gene
UseAAVExpressionMammalianAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
3xFlag-tdMCP-Erk2 (pErk reporter)
Plasmid#226167PurposeUsed to transiently express the pErk reporter in LABEL-seq MAPK activity assays.DepositorAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
miniTurbo-dCasRx
Plasmid#219821PurposeEF1α-driven expression of miniTurbo fused to dCasRx with GFP (Adapted from plasmid #153209)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCasRx
UseCRISPRTagsminiTurboExpressionMammalianPromoterEF1a and hU6Available sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV5.1-SARS-CoV-2 Nsp3
Plasmid#204205PurposeMammalian expression of SARS-CoV-2 Nsp3DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )
TagsFLAGx3ExpressionMammalianMutationnone, from obtained cloneAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE-BI-TRIM21
Plasmid#207838PurposeInducible TRIM21 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cellsDepositorInsertsTagsweak NLSExpressionMammalianMutationAll K mutated to RPromoterTRE3G BI promoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRE-BI-osTIR1
Plasmid#207840PurposeInducible TIR1 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cellsDepositorInsertsosTIR
mCherry
mAID_-VHH-GFP4
Tags3X HA-Tag and weak NLSExpressionMammalianMutationAll K mutated to RPromoterTRE3G BI promoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFUSE-rabbitFc1-IL2ss-VHH(G08)-His-Myc
Plasmid#209857PurposePlasmid coding for a nanobody (termed G08) against FGFR3 (mus musculus) with x6 HisTag, x3 myc, and Fabbit FC gamma 1DepositorInsertsIL2-ss
VHH (G08 variant)
Rabbit Fc Gamma 1
Tagsx3 MycTag and x6 HisTagExpressionMammalianPromoterhEF1-HTLV promoterAvailable sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-SIO-PdCO-mScarlet-WPRE
Plasmid#198509PurposeExpresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor after Cre-dependent recombination.DepositorInsertPdCO
UseAAV and Cre/LoxTagsRho1D4 and mScarletExpressionMammalianPromoterhSyn1Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-Mb-sfGFP WT
Plasmid#197569PurposeExpression of sfGFP WT with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertssfGFP WT
Pyl-tRNA (4 copies)
TagsV5-His6ExpressionMammalianPromoterCMV and U6/H1Available sinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-mCar-EGFP-WPRE-BGH-pA-UBC-Bla
Plasmid#174620PurposePiggyBac plasmid expressing EGFP under the control of a cone arrestin promoterDepositorInsertenhanced green fluorescent protein
UsePiggybacExpressionMammalianAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mScarlet-PuroR [M1G]
Plasmid#171809PurposeUniversal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and puromycin resistance cassette [p.M1G]DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmScarlet-T2A-PuroR
UseGene taggingMutationPuroR: Changed Methionin 1 to Glycine.Available sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hsp70l: hAPOB48-mCherry.
Plasmid#140639PurposeHeat shock promoter driven expression of human APOB48-mCherry. The construct has tol2 elements, and is designed to use in zebrafish.DepositorUseZebrafish expressionTagsmCherryMutationAPOB48: amino acid 1-2180Promoterhsp70lAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
U6_sgRNA_CAG_ABE7.10_St1Cas9_LMD9
Plasmid#136660PurposeExpresses ABE St1Cas9 LMD9 in mammalian cells along with its U6-driven sgRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsSt1Cas9 LMD-9
sgRNA for St1Cas9
ABE7.10
UseCRISPR and Synthetic BiologyTagsSV40 NLS and hSt1Cas9ExpressionMammalianPromoterCAG and U6Available sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEG302 22aa SunTag VP64 gRNA4 (FWA)
Plasmid#120249PurposeCRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNADepositorInsertg4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyExpressionPlantAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1(+)-sapphireAKAR(T/A)
Plasmid#118465PurposeNegative-control mutant for sapphireAKAR biosensor.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsapphireAKAR(T/A)
Tags6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
Pblu-AAVS1-Cas9-p300-M2rtTA-AAVS1
Plasmid#112261PurposeDonor plasmid with a Cas9-p300 expression cassette, a M2rtTA expression cassette, a T2A-puro selection cassette, and two gRNA recognition sequences at both ends of the whole insertion DNA fragment.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertspCas9-p300 (EP300 Synthetic, Human)
ExpressionMammalianAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
SIN-cPPT-H1-sgHTT1-U6-sgGFP2-7SK-sgCas9-PGK-mCherry-WPRE
Plasmid#87919PurposeHIV-1 SIN lentiviral transfer vectorDepositorInsertsgHTT1, shGFP2, sgCas9, cherry
UseLentiviralPromoterH1, U6, 7SK, PGKAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by