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pFRT-DHX36iso2

Plasmid

#159586

Purpose

Express N-ter FLAG HA tagged DHX36 isoform 2 in mammalian cells

Depositor

Markus Hafner

Article

Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

Insert

DHX36 (DHX36 Human)

Tags

FLAG HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pJS647

Plasmid

#183176

Purpose

PYR1 mutagenesis plasmid compatible with pJS646 Y2H vector

Depositor

Timothy Whitehead

Article

Beltran et al Nat Biotechnol. 2022 Jun 20. pii: 10.103

Insert

PYR1 (PYR1 Mustard Weed)

Expression

Bacterial

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF64-2xNSTREP

Plasmid

#136224

Purpose

Expresses N-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF64

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF64

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF26-2xCSTREP

Plasmid

#136186

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF26

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF26

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pTargetF-FRT

Plasmid

#113637

Purpose

Derivative of pTargetF with gRNA targeting FRT site

Depositor

Olivier Lichtarge

Article

Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

Insert

sgRNA-FRT

Use

CRISPR

Promoter

pJ23119

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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CRY2olig-mCh-CLC

Plasmid

#60035

Purpose

Expresses a fusion of CRY2olig (PHR, E490G) with mCherry and mouse clathrin light chain

Depositor

Chandra Tucker

Article

Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

Insert

CRY2-mCh-Clathrin Light Chain (CRY2 Mustard Weed, Mouse)

Expression

Mammalian

Mutation

M354I, E490G CRY2, A9D*

Promoter

CMV

Available Since

Oct. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pBADHISTEV_PfeE

Plasmid

#129120

Purpose

pBADHISTEV carrying pfeE for overexpression of PfeE in E. coli

Depositor

James Naismith

Article

Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

Insert

Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)

Tags

TEV protease cleavable 6xHis

Expression

Bacterial

Mutation

Signal peptide sequence has been removed

Promoter

PBAD

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-ArchT-GFP

Plasmid

#63141

Purpose

AAV-mediated expression of ArchT-GFP under the Syn promoter. Using hGHpA signal.

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Insert

ArchT-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

human synapsin promoter

Available Since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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pGreenII 0229/ProRUBY:GOXL1-Citrine:NOSt

Plasmid

#175563

Purpose

pSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-1

Depositor

George Haughn

Article

Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

Insert

GOXL1

Tags

Citrine

Expression

Plant

Promoter

RUBY

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCas9-sgVRK1 #4-Blast

Plasmid

#199647

Purpose

Expresses Cas9 and sgRNA guide targeting VRK1

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A (VRK1 Human)

Use

Lentiviral

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET28-MHL: nsp16_SARS-CoV-2

Plasmid

#167850

Purpose

Bacterial expression of the SARS-CoV-2 protein nsp16

Depositor

Cheryl Arrowsmith

Article

Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

Insert

nsp16

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 16, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pTol2p2A--3mnx1:loxP-mTagBFP2-stop-loxP-mCherry-NS-MCS

Plasmid

#78251

Purpose

Can be used to subclone in tagged gene of interest into inducible motor neuron expression vector.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Inserts

-3mnx1 (mnx1 Zebrafish)
-loxP-mTagBFP2-stop-
mCherry-NS-MCS

Use

Zebrafish motor neuron inducible expression

Promoter

-3mnx1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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FUT8-Dox

Plasmid

#124631

Purpose

Doxycycline-inducible FUT8 expression in mammalian cells

Depositor

Ron Weiss

Article

Chang et al Nat Chem Biol. 2019 May 20. pii: 10.1038

Inserts

Alpha-(1,6)-fucosyltransferase
Tetracycline repressor A3 mutant
TagFP635

Expression

Mammalian

Promoter

TRE-tight and hEF1a

Available Since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

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pNS38-SadCas9-SNAP

Plasmid

#113718

Purpose

Bacterial vector for expression of Snap-tagged Staphylococcus aureus dCas9

Depositor

Gerald Schwank

Article

Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

Insert

Cas9

Use

CRISPR

Tags

HA-2xNLS-SNAP-NLS and His6-MBP-TEV

Expression

Bacterial

Mutation

D10A, N580A

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF52-2xCSTREP

Plasmid

#136212

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF52

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF52

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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mDlx-BiPOLES-mCerulean

Plasmid

#154946

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Insert

BiPOLES

Use

AAV

Expression

Mammalian

Promoter

minimal Dlx

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF57-2xCSTREP

Plasmid

#136217

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF57

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF57

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF22-2xCSTREP

Plasmid

#136182

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF22

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF22

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF47-2xCSTREP

Plasmid

#136208

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF47

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF47

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-His:CRY2:nls:VPR

Plasmid

#135989

Purpose

Expresses the CRY2 blue light-inducible domain fused to the VPR transcriptional activation domain in mammalian cells

Depositor

Roman Jerala

Article

Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

Inserts

CRY2 domain fused to the VPR activation domain
CRY2 domain fused to the VPR activation domain

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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