We narrowed to 25,310 results for: expression vector

1. pLSU1/SCBV:nnLuz-I{PIV-216}:NosT

   Plasmid

   #212190

   Purpose

   This binary vector expresses mushroom luciferase (nnLuz) containing the PIV intron (216 bp into the gene) with the Sugarcane bacilliform virus (SCBV) promoter and UTR

   Depositor

   Wayne Curtis

   Insert

   nnLuz-I{PIV-216}
   

   Use

   Tags

   Expression

   Plant

   Mutation

   PIV intron added at bp 216, eliminates expression…

   Promoter

   Sugarcane Bacilliform Virus

   Available since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLSU1/o35s[]:mNeonGreen-I{PIV-198}:NosT

   Plasmid

   #212165

   Purpose

   This binary vector expresses mNeonGreen containing the PIV intron (198bp into the gene) with the truncated 35sCAMV promoter

   Depositor

   Wayne Curtis

   Insert

   Tomato codon optimized version of mNeonGreen-I{PIV-198}
   

   Use

   Tags

   Expression

   Plant

   Mutation

   mNeonGreen codon-optimized for tomato and PIV int…

   Promoter

   truncated 35sCaMV

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. DV.7.1

   Plasmid

   #208584

   Purpose

   Mammalian expression vector for expressing DV.7.1 SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncation

   Depositor

   Ben Murrell

   Insert

   SARS-CoV-2 variant DV.7.1 spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX6P1-DEST

   Plasmid

   #119749

   Purpose

   Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag.

   Depositor

   Martin Reijns, Andrew Jackson

   Type

   Empty backbone

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJMP12049

   Plasmid

   #222355

   Purpose

   Tn7 integrative vector with GFP expressed under PabstBR promoter and aprR marker on Tn7 transposon.

   Depositor

   Jason Peters

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PabstBR

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-alphaCaMKII-dsRed-Express-3XNLS-pA

   Plasmid

   #183802

   Purpose

   AAV vector to express nuclear localized dsRed-Express from CaMKIIa promoter

   Depositor

   Jonathan Ploski

   Article

   de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

   Insert

   dsRed-Express
   

   Use

   AAV

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEP4 E02S CK2M EN2L

   Plasmid

   #20924

   Purpose

   used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Klf4 and Myc; Nanog and Lin28

   Depositor

   James Thomson

   Article

   Yu et al Science. 2009 Mar 26.

   Insert

   Oct4 and Sox2; Nanog and Lin28; Klf4 and Myc (MYC, SOX2, KLF4, NANOG, POU5F1, LIN28A Human)
   

   Use

   Episomal/ebna

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 20, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-EF1alpha-dCas9-KRAB-MeCP2_Hygro

   Plasmid

   #192664

   Purpose

   3rd generation lenti vector encoding dCas9-KRAB-MeCP2 with 2A Hygro resistance marker

   Depositor

   Jeffrey Miner

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, D839A, H840A and N863A in Cas9

   Promoter

   EF1a

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. ORFtag_TetR-SD_Frame1

   Plasmid

   #220981

   Purpose

   ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame1

   Depositor

   Alexander Stark

   Insert

   TetR repressor
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK promoter

   Available since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. LentiCROP-PuroR-P2A-GFP-U6

    Plasmid

    #200029

    Purpose

    Lentiviral CROP-seq vector backbone with PuroR-P2A-GFP for cloning DNA Ticker Tapes and U6 for pegRNAs

    Depositor

    Jay Shendure

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    PuroR-P2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. xlox(GFP)hTERT

    Plasmid

    #69809

    Purpose

    MLV retroviral vector expressing the hTERT reverse transcriptase immortalizing gene and a GFP marker

    Depositor

    David Ott

    Insert

    hTERT Reverse Transcriptase (TERT )
    

    Use

    Retroviral

    Tags

    none

    Expression

    Mutation

    Promoter

    MLV promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. hSyn-DIO-somBiPOLES-eGFP

    Plasmid

    #192584

    Purpose

    AAV-vector for bidirectional optogenetic manipulation of Cre-positive neurons

    Depositor

    Simon Wiegert

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-EGFP-C3-cenexin

    Plasmid

    #73334

    Purpose

    Lentiviral vector encoding cenexin with an N-terminal EGFP tag

    Depositor

    Manuel Thery

    Insert

    cenexin/ODF2 (ODF2 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcU6.3_gRNA_c1

    Plasmid

    #221381

    Purpose

    Chicken-specific U6 sgRNA expression mini-vector with 10x capture sequence 1

    Depositor

    James Briscoe, Tatjana Sauka-Spengler

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    G. gallus U6.3

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUBC-Pfv-Sapphire

    Plasmid

    #203649

    Purpose

    Mammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the UBC promoter.

    Depositor

    Liam Holt

    Article

    Shu T et et al bioRxiv 2022

    Insert

    PfV
    

    Use

    Lentiviral

    Tags

    Sapphire

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. PGR-Blue

    Plasmid

    #68374

    Purpose

    Terminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.

    Depositor

    Nathan Reyna

    Type

    Empty backbone

    Use

    Synthetic Biology; Golden gate assembly

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDual_dsCas9_Blast_hs_NC_chr1

    Plasmid

    #214690

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)

    Depositor

    Kevin Janes

    Insert

    dgRNA_Chr1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDual_dsCas9_Blast_hs_TP73

    Plasmid

    #214691

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human TP73

    Depositor

    Kevin Janes

    Insert

    dgRNA_TP73 (TP73 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDD288

    Plasmid

    #137017

    Purpose

    mNG-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein, Daniel Dickinson

    Insert

    mNG-GLO^SEC^3xFlag + ccdB
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans GLO-optimized mNG

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLIX403-EBFP2-ccdB-G418

    Plasmid

    #158556

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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