2,325 results

1. pMRX-No-HaloTag7-mGFP-LC3

   Plasmid

   #184901

   Purpose

   Stable expression of HaloTag7-mGFP-LC3 in mammalian cells to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

   Insert

   LC3B
   

   Tags

   HaloTag7 and monomeric EGFP

   Expression

   Mammalian

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-DIO-rM3D(Gs)-mCherry

   Plasmid

   #50458

   Purpose

   Double floxed Gs-coupled rM3D DREADD fused with mCherry under the control of human synapsin promoter

   Depositor

   Bryan Roth

   Has Service

   AAV Retrograde and AAV8

   Insert

   rM3D-mCherry
   

   Use

   AAV

   Tags

   HA and mCherry

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   human Synapsin 1

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRX-No-HaloTag7-mGFP-LC3-mRFP

   Plasmid

   #184902

   Purpose

   Stable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

   Insert

   LC3B
   

   Tags

   HaloTag7, monomeric EGFP, and monomeric RFP

   Expression

   Mammalian

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGP-CMV-GCaMP6f

   Plasmid

   #40755

   Purpose

   Mammalian expression of ultrasensitive protein calcium sensor

   Depositor

   Douglas Kim, GENIE Project

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Insert

   GCaMP6f
   

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGP-CMV-NES-jRCaMP1a

   Plasmid

   #61562

   Purpose

   Red fluorescent calcium sensor protein

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Insert

   NES-jRCaMP1a
   

   Tags

   nuclear export signal

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lamp1-RFP

   Plasmid

   #1817

   Purpose

   Mammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.

   Depositor

   Walther Mothes

   Article

   Sherer et al Traffic 2003 Nov;4(11):785-801.

   Insert

   lysosome associated membrane protein 1 (Lamp1 Rat)
   

   Tags

   RFP

   Expression

   Mammalian

   Mutation

   D50E (not important for function of plasmid)

   Available Since

   Sept. 26, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMK293 (mAID-mCherry2-Hygro)

   Plasmid

   #72831

   Purpose

   C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-mCherry2-Hygro
   

   Use

   CRISPR

   Tags

   mAID-mCherry2

   Expression

   Mammalian

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. myc-mTOR

   Plasmid

   #1861

   Depositor

   David Sabatini

   Article

   Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

   Insert

   mTOR (Mtor Rat)
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   June 20, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-CamKII-TRPV1-P2A-mCherry

   Plasmid

   #200829

   Purpose

   Transduce excitatory neurons with AAV to express TRPV1 along with an mCherry fluorescence reporter

   Depositor

   Hong Chen

   Article

   Yang et al Brain Stimul. 2021 Jul-Aug;14(4):790-800

   Insert

   TRPV1-P2A-mCherry (Trpv1 Rat)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CamKII

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. psfGFP-LAMP1-mCherry (pHLARE)

    Plasmid

    #164477

    Purpose

    Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.

    Depositor

    Diane Barber

    Article

    Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

    Insert

    LAMP1 (Lamp1 Rat)
    

    Tags

    mCherry and superfolder GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. vGAT-pH pcaggs SE

    Plasmid

    #78578

    Purpose

    image recycling of VGAT in live neurons

    Depositor

    Susan Voglmaier

    Article

    Santos et al J Neurosci. 2013 Jun 26;33(26):10634-46.

    Insert

    vGAT-pHluorin C-term (Slc32a1 Rat)
    

    Tags

    superecliptic pHluorin

    Expression

    Mammalian

    Promoter

    chicken actin

    Available Since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pCAGGS-SGSM3[1-723]

    Plasmid

    #246258

    Purpose

    Rab5 GAP

    Depositor

    Ryohei Yasuda

    Article

    Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

    Insert

    Sgsm3[1-723] (Sgsm3 Rat)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPRIME-CMV-GFP-shNts2

    Plasmid

    #132714

    Purpose

    Encodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Nts

    Depositor

    Robert Messing

    Article

    Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

    Insert

    shNts(2) (Nts Rat)
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Oct. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-BE3

    Plasmid

    #73021

    Purpose

    Expresses BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

    Insert

    BE3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-lyso-pHoenix

    Plasmid

    #70112

    Purpose

    Expression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes

    Depositor

    Christian Rosenmund

    Article

    Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

    Insert

    lyso-pHoenix (Cd63 Rat, Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Mouse)
    

    Tags

    Arch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. Z-lock cofilin

    Plasmid

    #141137

    Purpose

    controlling the actin-severing ability of cofilin with light

    Depositor

    Klaus Hahn

    Article

    Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

    Insert

    Zdk2, Cofilin, LOV2
    

    Expression

    Mammalian

    Mutation

    mCherry, Zdk2(132F), Cofilin S3A, LOV WT

    Available Since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1 - PP1g - Flag - BirA* (AA2-140)

    Plasmid

    #86886

    Purpose

    N-terminal part of the split-BioID

    Depositor

    Mathieu Bollen

    Article

    Munter et al FEBS Lett. 2016 Dec 29. doi: 10.1002/187

    Insert

    PP1g - FLAG - BirA (AA2-140)
    

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    BirA Amino Acids 2-140

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-HA-rat Nrxn1alpha

    Plasmid

    #58266

    Purpose

    Expression plasmid for HA-tagged rat Neurexin 1alpha

    Depositor

    Peter Scheiffele

    Article

    Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

    Insert

    Nrxn1alpha (Nrxn1 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    see comments

    Promoter

    CAG

    Available Since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pVQ CMV NanoV1-2a-EGFP ferritin

    Plasmid

    #79649

    Purpose

    Expresses camelid anti-GFP nanobody fused to TRPV1 and GFP-ferritin chimera fusion protein

    Depositor

    Jeffrey Friedman

    Article

    Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do

    Inserts

    transient receptor potential vanillin 1 (Trpv1 Rat)
    camelid anti-GFP nanobody
    Enhanced green fluorescent protein
    mouse ferritin light chain (Ftl1 Mouse)
    mouse ferritin heavy chain (Fth1 Mouse)
    

    Use

    Adenoviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Ef1a-DIO_EGFP-GFE3

    Plasmid

    #79871

    Purpose

    For generating adeno-associated virus to express GFE3 under the EF1a promoter in a 'Cre-on' dependent manner.

    Depositor

    Don Arnold

    Article

    Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

    Has Service

    AAV1

    Insert

    GFP-GFE3
    

    Use

    AAV, Cre/Lox, and Synthetic Biology

    Tags

    GFP and HA

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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