2,326 results

1. Lamp1-RFP

   Plasmid

   #1817

   Purpose

   Mammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.

   Depositor

   Walther Mothes

   Article

   Sherer et al Traffic 2003 Nov;4(11):785-801.

   Insert

   lysosome associated membrane protein 1 (Lamp1 Rat)
   

   Tags

   RFP

   Expression

   Mammalian

   Mutation

   D50E (not important for function of plasmid)

   Available Since

   Sept. 26, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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2. psfGFP-LAMP1-mCherry (pHLARE)

   Plasmid

   #164477

   Purpose

   Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.

   Depositor

   Diane Barber

   Article

   Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

   Insert

   LAMP1 (Lamp1 Rat)
   

   Tags

   mCherry and superfolder GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-HA-rat Nrxn1alpha

   Plasmid

   #58266

   Purpose

   Expression plasmid for HA-tagged rat Neurexin 1alpha

   Depositor

   Peter Scheiffele

   Article

   Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

   Insert

   Nrxn1alpha (Nrxn1 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   see comments

   Promoter

   CAG

   Available Since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 - PP1g - Flag - BirA* (AA2-140)

   Plasmid

   #86886

   Purpose

   N-terminal part of the split-BioID

   Depositor

   Mathieu Bollen

   Article

   Munter et al FEBS Lett. 2016 Dec 29. doi: 10.1002/187

   Insert

   PP1g - FLAG - BirA (AA2-140)
   

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   BirA Amino Acids 2-140

   Available Since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Ef1a-DIO_EGFP-GFE3

   Plasmid

   #79871

   Purpose

   For generating adeno-associated virus to express GFE3 under the EF1a promoter in a 'Cre-on' dependent manner.

   Depositor

   Don Arnold

   Article

   Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

   Has Service

   AAV1

   Insert

   GFP-GFE3
   

   Use

   AAV, Cre/Lox, and Synthetic Biology

   Tags

   GFP and HA

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVQ CMV NanoV1-2a-EGFP ferritin

   Plasmid

   #79649

   Purpose

   Expresses camelid anti-GFP nanobody fused to TRPV1 and GFP-ferritin chimera fusion protein

   Depositor

   Jeffrey Friedman

   Article

   Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do

   Inserts

   transient receptor potential vanillin 1 (Trpv1 Rat)
   camelid anti-GFP nanobody
   Enhanced green fluorescent protein
   mouse ferritin light chain (Ftl1 Mouse)
   mouse ferritin heavy chain (Fth1 Mouse)
   

   Use

   Adenoviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-HA-rat Nrxn3beta AS4(-)

   Plasmid

   #58269

   Purpose

   Expression plasmid for HA-tagged rat Neurexin 3beta, lacking the insert at alternatively spliced segment 4 (AS4-)

   Depositor

   Peter Scheiffele

   Article

   Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

   Insert

   Nrxn3beta AS4(-) (Nrxn3 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-lyso-pHoenix

   Plasmid

   #70112

   Purpose

   Expression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes

   Depositor

   Christian Rosenmund

   Article

   Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

   Insert

   lyso-pHoenix (Cd63 Mouse, Rat, Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria)
   

   Tags

   Arch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. Z-lock cofilin

   Plasmid

   #141137

   Purpose

   controlling the actin-severing ability of cofilin with light

   Depositor

   Klaus Hahn

   Article

   Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

   Insert

   Zdk2, Cofilin, LOV2
   

   Expression

   Mammalian

   Mutation

   mCherry, Zdk2(132F), Cofilin S3A, LOV WT

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pORANGE Cacnb3-GFP KI

    Plasmid

    #139662

    Purpose

    Endogenous tagging of CaVβ3: C-terminal (amino acid position: W479)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Camui-CR

    Plasmid

    #40256

    Depositor

    Michael Lin

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Camuiα-CR
    

    Expression

    Mammalian

    Promoter

    CMV IE

    Available Since

    Oct. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-BE3

    Plasmid

    #73021

    Purpose

    Expresses BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

    Insert

    BE3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.hSyn.iGluSnFR3.v857.SGZ

    Plasmid

    #178330

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV hSyn iGluSnFR3 v857.SGZ
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and Stargazin and PDZ dom…

    Expression

    Mammalian

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-2dV-Camui (wt)

    Plasmid

    #220366

    Purpose

    Improved FLIM sensor for CaMK2-alpha activation (mammalian expression, cytosol)

    Depositor

    Ryohei Yasuda

    Article

    Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

    Insert

    mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(wt)-mEGFP(A206K) (Camk2a Rat, Synthetic)
    

    Tags

    mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-hSyn-RiboL1-jGCaMP8s

    Plasmid

    #169247

    Purpose

    AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8s protein.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Insert

    RiboL1-jGCaMP8s
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. SNAP-OMP25

    Plasmid

    #141149

    Purpose

    Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    OMP25 (Synj2bp Rat, Budding Yeast)
    

    Tags

    SNAP tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. msfGFP-Cx43

    Plasmid

    #69024

    Purpose

    Expresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to msfGFP (FP fused to NT of connexin). CMV promoter.

    Depositor

    David Spray

    Article

    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

    Insert

    Cx43 (Gja1 Rat)
    

    Tags

    sfGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28b-BE1

    Plasmid

    #73018

    Purpose

    Expresses BE1 with N-terminal His6 tag in E. Coli

    Depositor

    David Liu

    Article

    Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

    Insert

    BE1
    

    Tags

    His6 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFLAG ARMS

    Plasmid

    #24086

    Depositor

    Moses Chao

    Article

    Kong et al J Neurosci. 2001 Jan 1. 21(1):176-85.

    Insert

    ARMS (Kidins220 Rat)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-NR2B

    Plasmid

    #17925

    Depositor

    Stefano Vicini

    Article

    Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

    Insert

    N-methyl D-aspartate receptor 2B (Grin2b Rat)
    

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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