2,316 results

1. pCAG-2dV-Camui (T305D/T306D)

   Plasmid

   #220368

   Purpose

   T305D/T306D phosphomimetic mutant of pCAG-2dV-Camui

   Depositor

   Ryohei Yasuda

   Article

   Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

   Insert

   mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(T305D/T306D)-mEGFP(A206K) (Camk2a Rat, Synthetic)
   

   Tags

   mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

   Expression

   Mammalian

   Mutation

   changed both Threonine 305 and 306 to aspartic ac…

   Promoter

   CAG

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-2dV-Camui (T286A)

   Plasmid

   #220367

   Purpose

   T286A phosphodead mutant of pCAG-2dV-Camui

   Depositor

   Ryohei Yasuda

   Article

   Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

   Insert

   mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(T286A)-mEGFP(A206K) (Camk2a Rat, Synthetic)
   

   Tags

   mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

   Expression

   Mammalian

   Mutation

   changed Threonine 286 to Alanine

   Promoter

   CAG

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-hSyn-RiboL1-jGCaMP8s

   Plasmid

   #169247

   Purpose

   AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8s protein.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Insert

   RiboL1-jGCaMP8s
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCI pHluorin Syt12

   Plasmid

   #184513

   Purpose

   Mammalian expression of Synaptotagmin 12 with pH-sensitive fluorescent protein

   Depositor

   Edwin Chapman

   Article

   Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

   Insert

   Synaptotagmin 12 (Syt12 Rat)
   

   Tags

   Super Ecliptic pHluorin

   Expression

   Mammalian

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-SEP-Glur2 (MCA10)

   Plasmid

   #64941

   Purpose

   mammalian expression of rat GluR2

   Depositor

   Jeremy Henley

   Article

   Ashby et al J Neurosci. 2004 Jun 2;24(22):5172-6.

   Insert

   GluR2 (Gria2 Rat)
   

   Tags

   SEP (superecliptic pHluorin)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. beta-arrestin1 mYFP

   Plasmid

   #36916

   Depositor

   Robert Lefkowitz

   Article

   Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

   Insert

   beta-arrestin1 (Arrb1 Rat)
   

   Tags

   mYFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-BE3

   Plasmid

   #73021

   Purpose

   Expresses BE3 in mammalian cells

   Depositor

   David Liu

   Article

   Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

   Insert

   BE3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. C1A-C1A-EYFP

   Plasmid

   #61155

   Purpose

   Diacylglycerol (DAG) reporter

   Depositor

   Tobias Meyer

   Article

   Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

   Insert

   C1A-C1A-EYFP, C1A from PKCG (aa 26-89, rat) (Prkcg Rat)
   

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   C1A from PKCG amino acids 26-89

   Promoter

   CMV

   Available Since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. iRFP-PH-PLCdelta1

   Plasmid

   #66841

   Purpose

   expression of near-infrared PI(4,5)P2 biosensor

   Depositor

   Pietro De Camilli

   Article

   Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

   Insert

   PH-PLCdelta1
   

   Tags

   iRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CAG-H2BmGreenLantern

    Plasmid

    #177332

    Purpose

    To express a bright monomeric green FP to label eucaryotic cell nuclei. To be used in clearing and other fluorescent microscope methods.

    Depositor

    Murray Blackmore, Gregory Petsko, Pantelis Tsoulfas

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    H2B (Hist1h2bq Rat)
    

    Use

    AAV

    Tags

    mGreenLantern

    Expression

    Mammalian

    Mutation

    This fusion protein was optimized to the Human co…

    Promoter

    CAG

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-NR2B

    Plasmid

    #17925

    Depositor

    Stefano Vicini

    Article

    Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

    Insert

    N-methyl D-aspartate receptor 2B (Grin2b Rat)
    

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKT-CNP-inact

    Plasmid

    #211804

    Purpose

    Expresses inactive 2',3'-cyclic nucleotide phosphodiesterase catalytic domain (catalytic residues mutated)

    Depositor

    Emily Weinert

    Article

    Fontaine et al Biochem J. 2018 Apr 30;475(8):1491-1506.

    Insert

    2',3'-cyclic nucleotide phosphodiesterase catalytic domain (Cnp Rat)
    

    Expression

    Bacterial

    Mutation

    H73L H153L

    Promoter

    tet

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKT-CNP

    Plasmid

    #211803

    Purpose

    Expresses 2',3'-cyclic nucleotide phosphodiesterase catalytic domain

    Depositor

    Emily Weinert

    Article

    Fontaine et al Biochem J. 2018 Apr 30;475(8):1491-1506.

    Insert

    2',3'-cyclic nucleotide phosphodiesterase catalytic domain (Cnp Rat)
    

    Expression

    Bacterial

    Promoter

    tet

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMXs GFP-LC3-RFP

    Plasmid

    #117413

    Purpose

    Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux without any drug resistance.

    Depositor

    Noboru Mizushima

    Article

    Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Retroviral

    Tags

    EGFP and mRFP1

    Expression

    Mammalian

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV GFAP jGCaMP7b

    Plasmid

    #171118

    Purpose

    Astrocyte AAV-mediated expression of bright ultrasensitive protein calcium sensor under the GFAP promoter

    Depositor

    Thomas Oertner

    Article

    Lohr et al Front Cell Neurosci. 2021 Jun 11;15:6901

    Insert

    jGCaMP7b
    

    Use

    AAV

    Tags

    6 His Tag, T7 Tag, and Xpress Tag

    Expression

    Mammalian

    Promoter

    GFAP

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-HA-14-3-3 gamma

    Plasmid

    #13274

    Depositor

    Michael Yaffe

    Insert

    14-3-3 gamma (Ywhag Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-GCaMP5G

    Plasmid

    #31788

    Purpose

    a single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.

    Depositor

    Douglas Kim, Loren Looger, GENIE Project

    Article

    Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

    Insert

    GCaMP5G
    

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRS426GFP-2×PH(PLCδ)

    Plasmid

    #36092

    Depositor

    Scott Emr

    Article

    Stefan et al Mol Biol Cell. 2002 Feb;13(2):542-57.

    Insert

    2xPH(PLCdelta) fusion
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    contains aa11-140 of PLCδ (including the PH domai…

    Promoter

    PRC1

    Available Since

    June 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEF Synaptophysin-mRuby

    Plasmid

    #188980

    Purpose

    Synaptic buton marker

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Synaptophysin (Syp Rat, Synthetic)
    

    Expression

    Mammalian

    Available Since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLAMP1-emiRFP670

    Plasmid

    #136570

    Purpose

    LAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    LAMP1-emiRFP670
    

    Tags

    emiRFP670

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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