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pGP-CMV-NES-jRCaMP1a

Plasmid

#61562

Purpose

Red fluorescent calcium sensor protein

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Insert

NES-jRCaMP1a

Tags

nuclear export signal

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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myc-mTOR

Plasmid

#1861

Depositor

David Sabatini

Article

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

mTOR (Mtor Rat)

Tags

Myc

Expression

Mammalian

Available Since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

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pLenti-hPGK-eGFP-SynaptoZip

Plasmid

#122523

Purpose

Lentiviral expression of the synaptic activity-marker SynaptoZip fused to eGFP driven by hPGK promoter

Depositor

Antonio Malgaroli

Article

Ferro et al Nat Commun. 2017 Oct 31;8(1):1229. doi:

Insert

eGFP-SynaptoZip (Vamp2 Rat)

Use

Lentiviral

Tags

Myc and eGFP

Expression

Mammalian

Promoter

hPGK

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-eGFP-SynaptoZip

Plasmid

#122525

Purpose

AAV expression of the synaptic activity-marker SynaptoZip fused to eGFP driven by human Synapsin promoter

Depositor

Antonio Malgaroli

Article

Ferro et al Nat Commun. 2017 Oct 31;8(1):1229. doi:

Insert

eGFP-SynaptoZip (Vamp2 Rat)

Use

AAV

Tags

Myc and eGFP

Expression

Mammalian

Promoter

hSyn

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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Lamp1-RFP

Plasmid

#1817

Purpose

Mammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.

Depositor

Walther Mothes

Article

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

lysosome associated membrane protein 1 (Lamp1 Rat)

Tags

RFP

Expression

Mammalian

Mutation

D50E (not important for function of plasmid)

Available Since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

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pXL010-Wnt dual (GFP-Fire) reporter

Plasmid

#40588

Depositor

Sean Palecek

Article

Azarin et al Biomaterials. 2012 Mar;33(7):2041-9. Epu

Insert

Wnt reporter sequences

Use

Lentiviral

Expression

Mammalian

Promoter

Wnt promoter

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+):RGS4

Plasmid

#31157

Depositor

Henry Lester

Article

Doupnik et al Proc Natl Acad Sci U S A. 1997 Sep 16. 9

Insert

Regulator of G Protein Signaling 4 (Rgs4 Rat)

Expression

Mammalian

Available Since

Aug. 10, 2011

Availability

Academic Institutions and Nonprofits only
AP2u2-mCherry

Plasmid

#27672

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Ap2m1 (Ap2m1 Rat)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

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pEF SYT1-HaloTag

Plasmid

#197281

Purpose

Encodes full-length wild type synaptotagmin 1 (SYT1) with a C-terminal HaloTag

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

SYT1 and HaloTag (Syt1 Rat)

Expression

Mammalian

Mutation

N/A

Promoter

EF1alpha

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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LAMP1-Halo

Plasmid

#221655

Purpose

Lysosomal marker LAMP1 tagged C-terminally with Halo tag

Depositor

Erika Holzbaur

Article

Basak et al Nat Commun. 2025 Aug 9;16(1):7367. doi:

Insert

LAMP1 (Lamp1 Rat)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Rat SERCA1a (pMT2)

Plasmid

#75182

Purpose

Mammalian expression of ATP2A1

Depositor

Jonathan Lytton

Article

Wu et al Am J Physiol. 1993 Feb;264(2 Pt 1):C333-

Insert

ATP2A1

Expression

Mammalian

Promoter

adenovirus major late promoter

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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Rat SERCA2b (pMT2)

Plasmid

#75184

Purpose

Mammalian expression of ATP2A2

Depositor

Jonathan Lytton, Gary Shull

Article

Gunteski-Hamblin et al J Biol Chem. 1988 Oct 15;263(29):15032-4

Insert

ATP2A2

Expression

Mammalian

Promoter

adenovirus major late promoter

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP_TRPM8

Plasmid

#64879

Purpose

Expresses Ion Channel TRPM8 fused to eGFP in mammalian cells

Depositor

Sebastián Brauchi

Article

Toro et al J Neurosci. 2015 Jan 14;35(2):571-82. do

Insert

Transient receptor potential cation channel subfamily M member 8 (Trpm8 Rat)

Tags

eGFP

Available Since

June 13, 2016

Availability

Academic Institutions and Nonprofits only

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PSD-95 FLAG

Plasmid

#15463

Depositor

Wei-dong Yao

Article

Zhang et al J Biol Chem. 2007 May 25. 282(21):15778-

Insert

PSD-95 (Dlg4 Rat)

Tags

FLAG

Expression

Mammalian

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

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pET-hisMBP-rRNaseI

Plasmid

#216673

Purpose

Expresses hisMBP-tagged rat oxidation-resistant RNase inhibitor in E. coli hosts

Depositor

Po-Min Chiang

Article

Hsueh et al PLoS Biol. 2025 Dec 15;23(12):e3003537.

Insert

ribonuclease inhibitor (Rnh1 Rat)

Tags

his-tagged MBP

Expression

Bacterial

Promoter

T7

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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CaVα2δ1

Plasmid

#26575

Depositor

Diane Lipscombe

Article

Lin et al J Neurophysiol. 2004 Nov . 92(5):2820-30

Insert

Cacna2d1 (Cacna2d1 Rat)

Expression

Mammalian

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

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mClY-N1

Plasmid

#90457

Purpose

A YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.

Depositor

Joseph Santos-Sacchi

Article

Zhong et al PLoS One. 2014 Jun 5;9(6):e99095. doi: 1

Insert

mClY-N1

Expression

Mammalian

Mutation

EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP6f

Plasmid

#40755

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Insert

GCaMP6f

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-RiboL1-jGCaMP8s

Plasmid

#169247

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8s protein.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8s

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

hSyn

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pHTN-LC3B

Plasmid

#219440

Purpose

Expresses HaloTag-LC3B, an autophagosome marker

Depositor

Erika Holzbaur

Article

Tudorica et al J Cell Biol. 2024 Jul 1;223(7):e20230901

Insert

LC3B (Map1lc3b Rat)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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