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Showing: 121 - 160 of 312 results

  1. R777-E166 Hs.PIK3CB-nostop

    Plasmid
    #70450
    Purpose
    Gateway ORF clone of human PIK3CB [NM_006219.2] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PIK3CB (PIK3CB Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70450-plasmid-map-sequence-id-358325

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  2. pET3-ARL8A-His6

    Plasmid
    #201872
    Purpose
    Used for bacterial expression of human protein ARL8A
    Depositor
    Richard Kahn
    Insert
    ARL8A (ARL8A Human)
    Use
    Tags
    6xHis (GSGSGSHHHHHH)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    201872-plasmid-map-sequence-id-403021

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  4. pCAG-Scarlet-Kif5a

    Plasmid
    #118745
    Purpose
    Expresses scarlet tagged Kif5a
    Depositor
    Beverly Koller
    Insert
    Kif5a cDNA (Kif5a Mouse)
    Use
    Tags
    mScarlet
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    118745-plasmid-map-sequence-id-229089

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  5. pScarlet-Kif5a

    Plasmid
    #118744
    Purpose
    Expresses scarlet tagged Kif5a
    Depositor
    Beverly Koller
    Insert
    Kif5a cDNA (Kif5a Mouse)
    Use
    Tags
    mScarlet
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    118744-plasmid-map-sequence-id-229075

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  6. pAS_MmaPylT_EF1_NES-MmaPylRS WT

    Plasmid
    #174901
    Purpose
    expression of Mma PylRS with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration
    Depositor
    Simon Elsaesser
    Insert
    Mma PylRS (pylS Methanosarcina mazei)
    Use
    Tags
    FLAG and NES
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    174901-plasmid-map-sequence-id-348003

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  7. pAS_MmaPylT_EF1_NES-MmaPylRS AF

    Plasmid
    #174902
    Purpose
    expression of Mma PylRS AF with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration
    Depositor
    Simon Elsaesser
    Insert
    Mma PylRS (pylS Methanosarcina mazei)
    Use
    Tags
    FLAG and NES
    Expression
    Mammalian
    Mutation
    Y306A, Y384F
    Promoter
    EF1
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    174902-plasmid-map-sequence-id-344602

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  8. pFastBac Dual PNG172-STII PLU-HIS

    Plasmid
    #112992
    Purpose
    Baculovirus expression of Drosophila png172-Flag and plu-His
    Depositor
    Terry Orr-Weaver
    Inserts
    png172 (png Fly)
    plu (plu Fly)
    Use
    Tags
    Flag and His
    Expression
    Insect
    Mutation
    png172 mutation
    Promoter
    Available since
    Aug. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    112992-plasmid-map-sequence-id-217463

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  9. pFastBac Dual PNG-STII PLU-HIS

    Plasmid
    #112993
    Purpose
    Baculovirus expression of Drosophila png-Flag and plu-His
    Depositor
    Terry Orr-Weaver
    Inserts
    png (png Fly)
    plu (plu Fly)
    Use
    Tags
    Flag and His
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    112993-plasmid-map-sequence-id-217440

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  14. MK1283

    Plasmid
    #71428
    Purpose
    This E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 1535 and a TetR translation rate of 30709
    Depositor
    Howard Salis
    Article
    Kushwaha et al Nat Commun. 2015 Jul 17;6:7832. doi: 10.
    Insert
    Mixed Feedback Loop version of the UBER system
    Use
    Tags
    Expression
    Mutation
    T7 RBS: AACCGAGCCCAATATAGGACCTAGGGTGCCAAAAAA and …
    Promoter
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71428-plasmid-map-sequence-id-440608

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  16. MK1274

    Plasmid
    #71431
    Purpose
    This E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 300 and a TetR translation rate of 35149.
    Depositor
    Howard Salis
    Article
    Kushwaha et al Nat Commun. 2015 Jul 17;6:7832. doi: 10.
    Insert
    Mixed Feedback Loop version of the UBER system
    Use
    Tags
    Expression
    Mutation
    T7 RBS:AACCGAGCCCAATATAGGACTCAGGGTGCCAAAAAA and T…
    Promoter
    Available since
    Dec. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    71431-plasmid-map-sequence-id-197095

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  25. pTrc-trGPPS(CO)-LS

    Plasmid
    #50603
    Purpose
    pTrc-trGPPS(CO)-LS: A plasmid that has truncated codon optimized geranylpyrophosphate synthase and truncated limonene synthase
    Depositor
    Jay Keasling
    Article
    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1
    Inserts
    Limonene Synthase
    ApR
    LacIq
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    truncated codon optimized geranylpyrophosphate sy…
    Promoter
    Trc
    Available since
    July 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    50603-plasmid-map-sequence-id-77114

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  28. pR6Kg-Omega1

    Plasmid
    #165864
    Purpose
    Omega level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    165864-plasmid-map-sequence-id-335014

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  29. pR6Kg-Alpha1

    Plasmid
    #165862
    Purpose
    Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    165862-plasmid-map-sequence-id-335009

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  34. pCMK83

    Plasmid
    #226793
    Purpose
    Integrates slr0214 (HIP1 methyltransferase) into gidB-atpI locus of E. coli DH10B
    Depositor
    David Nielsen
    Article
    Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:
    Insert
    slr0214 (slr0214 Synechocystis sp. PCC 6803)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Tetracycline resistance cassette promoter (consti…
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    226793-plasmid-map-sequence-id-455428

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  39. pGal4x5-E4T-G210

    Plasmid
    #171077
    Purpose
    The resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permission
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Insert
    Gal4-E4-Gless-cassette
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171077-plasmid-map-sequence-id-335554

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Showing: 121 - 160 of 312 results