We narrowed to 733 results for: Tcta

1. pKDsgRNA-FRT

   Plasmid

   #183241

   Purpose

   anhydrotetracycline (aTC) inducible sgRNA that targets FRT scar sites and arabinose inducible lambda Red

   Depositor

   Karin Schnetz

   Article

   Rangarajan et al Microbiology (Reading). 2022 Apr;168(4).

   Insert

   sgRNA-FRT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P-tet

   Available Since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330.iGFP2

   Plasmid

   #66582

   Purpose

   sgRNA for iGFP

   Depositor

   Wen Xue

   Article

   Li et al Genome Biol. 2015 May 28;16(1):111.

   Insert

   iGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pBSU6-shTOM40.3

   Plasmid

   #89796

   Purpose

   Expresses shRNA against TOM40.

   Depositor

   Robert Friedlander

   Article

   Yano et al Nat Neurosci. 2014 Jun;17(6):822-31. doi

   Insert

   shRNA Tom40
   

   Use

   Tags

   none

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1P shISCU

   Plasmid

   #102972

   Purpose

   Suppression of ISCU

   Depositor

   Richard Possemato

   Article

   Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

   Insert

   shISCU
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJRL2 PHO5(TATA-C2)pr ECFP (EB#1562)

   Plasmid

   #14844

   Depositor

   Erin O'Shea

   Article

   Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

   Insert

   PHO5 promoter TATA-C2 (PHO5 Budding Yeast)
   

   Use

   Tags

   ECFP

   Expression

   Yeast

   Mutation

   TATA-C2. TATA box mutated from TATATAAG to TCTA…

   Promoter

   Available Since

   May 25, 2007

   Availability

   Academic Institutions and Nonprofits only

6. PX458_THRA_iso1_1

   Plasmid

   #128245

   Purpose

   Encodes gRNA for 3' target of human THRA_iso1

   Depositor

   Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   THRA_iso1 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. PX458_ZBTB49_iso1_1

   Plasmid

   #128250

   Purpose

   Encodes gRNA for 3' target of human ZBTB49_iso1

   Depositor

   Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   ZBTB49_iso1 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pE2F5.1.0-gDNA

   Plasmid

   #112447

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor E2F5

   Depositor

   Kevin White

   Insert

   E2F5 (E2F5 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pWT029g

   Plasmid

   #96860

   Purpose

   guanine-agRNA (GFP activation, 18 nt blocker with bulges)

   Depositor

   David Liu

   Article

   Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

   Insert

   guanine-agRNA (GFP activation, 18 nt blocker with bulges)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pWT029h

    Plasmid

    #96861

    Purpose

    (d)guanine-agRNA (GFP activation,18 nt blocker with bulges)

    Depositor

    David Liu

    Article

    Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

    Insert

    (d)guanine-agRNA (GFP activation,18 nt blocker with bulges)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pWT049d

    Plasmid

    #96862

    Purpose

    guanine-agRNA (19 nt blocker with bulges, RFP activation)

    Depositor

    David Liu

    Article

    Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

    Insert

    guanine-agRNA (19 nt blocker with bulges, RFP activation)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pWT049e

    Plasmid

    #96863

    Purpose

    (d)guanine-agRNA (19 nt blocker with bulges, RFP activation)

    Depositor

    David Liu

    Article

    Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

    Insert

    (d)guanine-agRNA (19 nt blocker with bulges, RFP activation)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJRL2 GAL1(TATA-C2)pr VYFP (EB#1577)

    Plasmid

    #14851

    Depositor

    Erin O'Shea

    Article

    Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

    Insert

    GAL1 promoter TATA-C2 (GAL1 Budding Yeast)
    

    Use

    Tags

    VYFP

    Expression

    Yeast

    Mutation

    TATA-C2. TATA box mutated from TATATAAG to TCTATA…

    Promoter

    Available Since

    May 25, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pJRL2 GAL1(TATA-C2)pr ECFP (EB#1578)

    Plasmid

    #14852

    Depositor

    Erin O'Shea

    Article

    Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

    Insert

    GAL1 promoter TATA-C2 (GAL1 Budding Yeast)
    

    Use

    Tags

    ECFP

    Expression

    Yeast

    Mutation

    TATA-C2. TATA box mutated from TATATAAG to TCTATA…

    Promoter

    Available Since

    May 25, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1+-Tornado-nLuc

    Plasmid

    #212611

    Purpose

    For expression of a circular nLuc mRNA. To express your gene of interest clone into the BsiWI, SacII sites and add the GCCATGTcTATGTGG 5' of the SacII site.

    Depositor

    Samie Jaffrey

    Article

    Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

    Insert

    Tornado-CVB3-nLuc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-dCas9-KRAB-gRNA-TRE-blast

    Plasmid

    #201152

    Purpose

    Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Inserts

    dCas9-KRAB
    gRNA-TRE
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mutation

    gRNA sequence: TACGTTCTCTATCACTGAT

    Promoter

    Available Since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJYS2_crtYf

    Plasmid

    #85544

    Purpose

    Constitutive transcription of crRNA of crtYf, Spectinomycin resistance

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Insert

    crRNA of crtYf
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCR-U6-gRNA-TRE3G-TdTomato

    Plasmid

    #192658

    Purpose

    A plasmid encoding TRE3G sgRNA and TRE3G-promoter-TdTomato

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    TdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, TRE3G

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX459_gRNA-ROSA26_hspCas9

    Plasmid

    #193310

    Purpose

    Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

    Insert

    Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    not applicable

    Promoter

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLH-stsgRNA3.1

    Plasmid

    #64118

    Purpose

    Vector for expression of St1 sgRNA3.1 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 sgRNA3.1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJYS2cm_crtYf

    Plasmid

    #85543

    Purpose

    Constitutive transcription of crRNA of crtYf, Chloramphenicol resistance

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Insert

    crRNA of crtYf
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTarget12f1

    Plasmid

    #171183

    Purpose

    Constitutive expression of sgRNA without donor DNA

    Depositor

    Kohsuke Honda

    Article

    Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

    Insert

    sgRNA scaffold in the CRISPR/Cas12f1 system
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119(SpeI) promoter

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSLQ1870-1 pHR: U6-SpsgTRE3G CMV-mCherry

    Plasmid

    #84248

    Purpose

    Expresses Sp sgTRE3G gRNA with a mCherry fluorescent marker

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Insert

    Sp sgTRE3G
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MK1283

    Plasmid

    #71428

    Purpose

    This E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 1535 and a TetR translation rate of 30709

    Depositor

    Howard Salis

    Article

    Kushwaha et al Nat Commun. 2015 Jul 17;6:7832. doi: 10.

    Insert

    Mixed Feedback Loop version of the UBER system
    

    Use

    Tags

    Expression

    Mutation

    T7 RBS: AACCGAGCCCAATATAGGACCTAGGGTGCCAAAAAA and …

    Promoter

    Available Since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. MK1274

    Plasmid

    #71431

    Purpose

    This E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 300 and a TetR translation rate of 35149.

    Depositor

    Howard Salis

    Article

    Kushwaha et al Nat Commun. 2015 Jul 17;6:7832. doi: 10.

    Insert

    Mixed Feedback Loop version of the UBER system
    

    Use

    Tags

    Expression

    Mutation

    T7 RBS:AACCGAGCCCAATATAGGACTCAGGGTGCCAAAAAA and T…

    Promoter

    Available Since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJZC603

    Plasmid

    #62317

    Purpose

    sgRNA with 2x PP7 for yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA + 2x PP7 RNA binding module
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLKO-Tet-On-HDAC1-shRNA2

    Plasmid

    #90241

    Purpose

    Lentivirus for expression of shRNA2 against human HDAC1 (Dox-inducible)

    Depositor

    Roland Friedel

    Insert

    HDAC1 (HDAC1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJZC545

    Plasmid

    #62313

    Purpose

    sgRNA with 1x MS2 for yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA + 1x MS2 binding module
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pUC19-U6-AksgRNA

    Plasmid

    #121955

    Purpose

    Mammalian expression, Genome editing, gRNA scaffold

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AkCas12b single chimeric gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pUC19-U6-AasgRNA

    Plasmid

    #121954

    Purpose

    Mammalian expression, Genome editing, gRNA scaffold

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AaCas12b single chimeric gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pUC19-U6-AasgRNA3.8

    Plasmid

    #121958

    Purpose

    Mammalian expression, Genome editing, gRNA scaffold

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AaCas12b single chimeric gRNA, short version
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLH-stsgRNA2.1

    Plasmid

    #64117

    Purpose

    Vector for expression of St1 sgRNA2.1 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 sgRNA2.1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMD19T-slr0230-PL22-sgRNANT1-KmR

    Plasmid

    #73224

    Purpose

    Contains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coli

    Depositor

    Paul Hudson

    Article

    Yao et al ACS Synth Biol. 2015 Dec 28.

    Insert

    PL22-sgRNA NT1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PL22

    Available Since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJZC583

    Plasmid

    #62314

    Purpose

    sgRNA with 2x MS2 for yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA + 2x MS2 binding module
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available Since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLH-stsgRNA1.1

    Plasmid

    #64116

    Purpose

    Vector for expression of St1 sgRNA1.1 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 sgRNA1.1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJZC572

    Plasmid

    #62318

    Purpose

    sgRNA with 1x com for yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA + 1x com RNA binding module
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pmU6 ST

    Plasmid

    #188713

    Purpose

    sgRNA plasmid encoding mU6 promoter driving an anti-safe targeting sgRNA

    Depositor

    David Tobin

    Article

    Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

    Insert

    Safe Targeting sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pUC19-U6-crRNA1

    Plasmid

    #123639

    Purpose

    Mammalian expression, Genome editing, gRNA scaffold

    Depositor

    Wei Li

    Article

    Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

    Insert

    crRNA scaffold 1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pU6_(GLuc)_sgRNA

    Plasmid

    #68422

    Purpose

    Transient expression of a minimal sgRNA targeting the GLuc reporter, in mammalian cells. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    Gluc sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLL3.7 Lmo4.3 shRNA

    Plasmid

    #59292

    Purpose

    shRNA to mouse Lmo4

    Depositor

    Ulrike Heberlein

    Article

    Lasek et al Genes Brain Behav. 2010 Oct;9(7):817-24.

    Insert

    Lmo4.3 shRNA
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available Since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only