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pET24_NusA-Tus
Plasmid#165959PurposeExpression of soluble E. coli Tus proteinDepositorInsertTus
UseTagsHis-NusA-TevExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD24-fis
Plasmid#51557PurposeE. coli fis ORF cloned into pBAD24 expression vectorDepositorInsertFIS (fis E. coli)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJ211_WT_low
Plasmid#128848PurposeLow copy number plasmid for expression of E. coli flagellin from its natural promoterDepositorInsertFlagellin
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ211_WT_hi
Plasmid#128850PurposeHigh copy number plasmid for expression of E. coli flagellin from its natural promoterDepositorInsertFlagellin
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJB066
Plasmid#98913PurposeExpress FtsZ tagged with pAmCherry1 in E coliDepositorInsertFtsZ
UseTagspAmCherry1ExpressionBacterialMutationPromoterpBADAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJ291_WT_med
Plasmid#128856PurposeMedium copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoterDepositorInsertFlagellin
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEV_S
Plasmid#100266PurposepDSW204 carrying genes entABCDE, vibHF and a cos site, for serratiochelin and analogs production in E. coliDepositorInsertsUseSynthetic Biology; Expression, pbr322TagsExpressionBacterialMutationPromoterAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJ291_WT_low
Plasmid#128855PurposeLow copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoterDepositorInsertFlagellin
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ211_rha_WT_low
Plasmid#128851PurposeLow copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoterDepositorInsertFlagellin
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ211_rha_WT_med
Plasmid#128852PurposeMedium copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoterDepositorInsertFlagellin
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
Strep-TGT-His
Plasmid#206495PurposeExpresses E. Coli TGT with an N-terminal Strep Tag and a C-terminal His TagDepositorInsertE. coli tRNA Guanine Transglycosylase
UseTagsHis and StrepExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJ211_WT_med
Plasmid#128849PurposeMedium copy number plasmid for expression of E. coli flagellin from its natural promoterDepositorInsertFlagellin
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ211_rha_WT_hi
Plasmid#128853PurposeHigh copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoterDepositorInsertFlagellin
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
SSB-wt
Plasmid#197260Purposewild type E. coli SSB in pET21 expresses in BL21 under T7 control with lactose-amp resistanceDepositorInsertwt E. coli SSB
UseTagsnoneExpressionBacterialMutationPromoterT7Available sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACM4-ACA
Plasmid#49809PurposeEncodes fabD, accA, accB, accC, accD in pseudo-operon configuration.DepositorInsertsACP transacylase
Acetyl-CoA carboxylase
acetyl-CoA carboxylase
Acetyl-CoA carboxylase
Acetyl-CoA carboxylase
UseTagsExpressionBacterialMutationSilent site-directed mutagenesis to remove SalI s…PromoterT7Available sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCK314
Plasmid#110545PurposeModified (delta CRP-binding site) rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteriaDepositorInsertsModified PrhaBAD (CRP-binding sites removed)
yfp
rhaS
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEcRNAP6
Plasmid#128940PurposepET21a derivative encoding E. coli core RNA polymerase subunits: RpoA, B, C-ppx(His)10, ZDepositorInsertsUseTagsHisExpressionBacterialMutationPreScission protease site (GE Healthcare; LEVLFQG…PromoterT7Available sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
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His8:MBP-tev-Gly-eK-Flv
Plasmid#83383Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Trp-eK-Flv
Plasmid#83390Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Asp-eK-Flv
Plasmid#83389Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-His-eK-Flv
Plasmid#83388Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Lys-eK-Flv
Plasmid#83387Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Leu-eK-Flv
Plasmid#83386Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Arg-eK-Flv
Plasmid#83385Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Phe-eK-Flv
Plasmid#83384Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCK306
Plasmid#110544PurposerhaBAD, a rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria.DepositorInsertsPrhaBAD
yfp
rhaS
UseTagsExpressionBacterialMutationPromoterN/A, PrhaBAD, and kanR promoter (upstream of kanR…Available sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
JM3 [JBEI-6891]
Plasmid#89788PurposeBroad host range vector pBBR1-MCS2 containing 'tesA (E. coli), fadB (E. coli), Mlut_11700 (M. luteus) and fadM (E. coli) under control of BAD promoterDepositorInserttesA (E. coli), fadB (E. coli), Mlut_11700 (M. luteus) and fadM (E. coli)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pM1s3AsG
Plasmid#137921PurposeE. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive GFP expressionDepositorInsertSuperfolder GFP
UseSynthetic Biology; For use with e. coli nissle in…TagsExpressionBacterialMutationPromoterJ23101Available sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBP-lldR
Plasmid#74095PurposeL-lactate repressor (LldR) from E. coli MG1655DepositorInsertlldR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterNoneAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pM2s2AsG
Plasmid#137924PurposeE. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive GFP expressionDepositorInsertSuperfolder GFP
UseSynthetic Biology; For use with e. coli nissle in…TagsExpressionBacterialMutationPromoterJ23101Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTJV1Sc3-rpoB
Plasmid#166983PurposeGenerates ssDNA in vivo targeting E. coli rpoB for recombineering w/ EcRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistanceDepositorInsertRecT (recT E. coli)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterpVvanCCAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM6-HpaBC
Plasmid#71260PurposeExpresses E. coli HpaBC in monocistronic form from ePathBrick vector, pETM6DepositorInsertsHpaB
HpaC
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pM1s3AsR_TS-C7
Plasmid#137945PurposeE. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive RFP expression and strong temperature sensitive GFP expressionDepositorInsertmRFP1
UseSynthetic Biology; For use with e. coli nissle in…TagsExpressionBacterialMutationPromoterJ23101Available sinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046852
Plasmid#145584PurposeBacterial Expression plasmid for SARS-CoV-2 nsp8DepositorInsertSARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pM1s3ATScsg-Etag
Plasmid#137946PurposeE. coli Nissle pMUT1-derived plasmid with ampicillin resistance and temperature sensitive curli expressionDepositorInsertsSynthetic curli operon
Synthetic curli operon
UseSynthetic Biology; For use with e. coli nissle in…TagsExpressionBacterialMutationPromoterpTlpA36Available sinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046979
Plasmid#145611PurposeBacterial Expression plasmid for SARS-CoV-2 nsp7DepositorInsertSARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046920
Plasmid#145717PurposeBacterial Expression plasmid for SARS-CoV-2 nsp3DepositorInsertSARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046760
Plasmid#145600PurposeBacterial Expression plasmid for SARS-CoV-2 helicaseDepositorInsertSARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046740
Plasmid#145588PurposeBacterial Expression plasmid for SARS-CoV-2 endoRNAseDepositorInsertSARS-CoV-2 endoRNAse (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046916
Plasmid#145627PurposeBacterial Expression plasmid for SARS-CoV-2 nsp6DepositorInsertSARS-CoV-2 nsp6 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046731
Plasmid#145633PurposeBacterial Expression plasmid for SARS-CoV-2 nsp2DepositorInsertSARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046871
Plasmid#145724PurposeBacterial Expression plasmid for SARS-CoV-2 nsp10DepositorInsertSARS-CoV-2 nsp10 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046833
Plasmid#145649PurposeBacterial Expression plasmid for SARS-CoV-2 nsp9DepositorInsertSARS-CoV-2 nsp9 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRnc-gGFP161
Plasmid#189536PurposeExpresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-161-GFPDepositorInsertsRNase III
gUTR-161-GFP
UseTagsExpressionBacterialMutationN/APromoterJ23119 and pBADAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJB089
Plasmid#98914PurposeExpress Dronpa tagged ZapA and pAmCherry1 tagged FtsZ in E coliDepositorInsertsFtsZ
ZapA
UseTagsDronpa and pAmCherry1ExpressionBacterialMutationPromoterT5-lacAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046901
Plasmid#145725PurposeBacterial Expression plasmid for SARS-CoV-2 ORF7a proteinDepositorInsertSARS-CoV-2 ORF7a protein (ORF7a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046854
Plasmid#145759PurposeBacterial Expression plasmid for SARS-CoV-2 leader proteinDepositorInsertSARS-CoV-2 leader protein (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046832
Plasmid#145691PurposeBacterial Expression plasmid for SARS-CoV-2 nsp7DepositorInsertSARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;StrepIIExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits
