We narrowed to 9,389 results for: Coli
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Plasmid#183841PurposesfGFP reporter under the control of Mutant E. coli thiB TPP Riboswitch under the control of a Constitutive promoterDepositorInsertE. coli thiB TPP Riboswitch (U66A) regulated sfGFP
UseTagsExpressionBacterialMutationPromoterJ23119 - Anderson PromoterAvailable sinceAug. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
JBL6405
Plasmid#183842PurposesfGFP reporter under the control of Mutant E. coli thiB TPP Riboswitch under the control of a Constitutive promoterDepositorInsertE. coli thiB TPP Riboswitch (G76C) regulated sfGFP
UseTagsExpressionBacterialMutationPromoterJ23119 - Anderson PromoterAvailable sinceAug. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-28b(+)/ADK C77S
Plasmid#201409PurposeOverexpresses Escherichia coli adenylate kinase C77SDepositorInsertEscherichia coli adenylate kinase C77S (adk Escherichia coli)
UseTagsExpressionBacterialMutationchanged cysteine 77 to serinePromoterAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHflu4
Plasmid#203882PurposepHI1 with insertion of monomeric red fluorescent protein (mRFP)DepositorInsertmonomeric red fluorescent protein
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSucCD2
Plasmid#83324Purposeexpresses E. coli succinyl-CoA synthetaseDepositorInsertsucCD
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-53-RtcB
Plasmid#51282PurposeE. coli expression vector for E. coli RtcB RNA ligaseDepositorInsertE. coli RtcB
UseTags6xHisExpressionBacterialMutationPromoterT7 RNA PolymeraseAvailable sinceMarch 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOSIP-KL-mcherry
Plasmid#116947PurposeIntegrating mCherry into lambda attB in E. coli chromosome.DepositorInsertmcherry
UseTagsExpressionMutationPromoterAvailable sinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEG03
Plasmid#154138PurposeThis plasmid carries fucO and aldA under the control of P_gyrA promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.DepositorInsertfucO, aldA
UseTagsExpressionBacterialMutationPromoterP_gyrAAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEG03'
Plasmid#162578PurposeThis plasmid carries fucO and aldA under the control of P_gyrA promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.DepositorInsertfucO, aldA
UseTagsExpressionBacterialMutationPromoterP_gyrAAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK057
Plasmid#75036PurposeProduces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase, N-terminal His6 (H6EcPurE1) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)DepositorInsertsN5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase (purK Escherichia coli)
UseTagsExpressionBacterialMutationchanges residues 2-7 to His (SSRNNP>HHHHHH)PromoterT7 and n/aAvailable sinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEG01
Plasmid#154136PurposeThis plasmid carries fucO and aldA under the control of P_BAD promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.DepositorInsertfucO, aldA
UseTagsExpressionBacterialMutationPromoterP_BADAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEG02
Plasmid#154137PurposeThis plasmid carries fucO and aldA under the control of P_thrC3 promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.DepositorInsertfucO, aldA
UseTagsExpressionBacterialMutationPromoterP_thrC3Available sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCK351
Plasmid#129696PurposeFor insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120034435DepositorInsertsPrhaBAD
yfp
rhaS
Terminator ECK120034435
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCK354
Plasmid#129698PurposeFor insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120010799DepositorInsertsPrhaBAD
yfp
rhaS
Terminator ECK120010799
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCK353
Plasmid#129697PurposeFor insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120015170DepositorInsertsPrhaBAD
yfp
rhaS
Terminator ECK120015170
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCK355
Plasmid#129699PurposeFor insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + ilvBN terminatorDepositorInsertsPrhaBAD
yfp
rhaS
ilvBN terminator
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHflu2
Plasmid#203880PurposepHI1 with insertion of ampicillin-resistance geneDepositorInsertbroad-spectrum class A beta-lactamase TEM-116
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHflu3
Plasmid#203881PurposepHI1 with insertion of kanamycin-resistance geneDepositorInsertaminoglycoside O-phosphotransferase APH(3')-IIa
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZA31-sulA-GFP
Plasmid#78493PurposeExpression of GFP under the sulA promoter.DepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet-1_Ec_rpoZ
Plasmid#128837PurposeE. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.DepositorInsertrpoZ (rpoZ E. coli)
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXY027
Plasmid#98915PurposeExpress FtsZ tagged with GFP in E coliDepositorInsertFtsZ
UseTagsGFPExpressionBacterialMutationPromoterT5-lacAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD.Sigma32.I54N
Plasmid#59982PurposeExpresses sigma32 I54N mutant in E. coli to render a heat-shock like cellular folding environmentDepositorInsertSigma32 I54N
UseTagsExpressionBacterialMutationI54NPromoterAvailable sinceNov. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET24_NusA-Tus
Plasmid#165959PurposeExpression of soluble E. coli Tus proteinDepositorInsertTus
UseTagsHis-NusA-TevExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pM2s2TsR
Plasmid#137923PurposeE. coli Nissle pMUT2-derived plasmid with tetracycline resistance and constitutive RFP expressionDepositorInsertmRFP1
UseSynthetic Biology; For use with e. coli nissle in…TagsExpressionBacterialMutationPromoterJ23101Available sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pM1s3TsR
Plasmid#137922PurposeE. coli Nissle pMUT1-derived plasmid with tetracycline resistance and constitutive RFP expressionDepositorInsertmRFP1
UseSynthetic Biology; For use with e. coli nissle in…TagsExpressionBacterialMutationPromoterJ23101Available sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pD441_WT_hi
Plasmid#128857PurposeHigh copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoterDepositorInsertFlagellin
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKB817 (pBra-Hfq)
Plasmid#174661PurposePlpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli Hfq. Useful as three-hybrid positive control.DepositorInsertHfq
UseTagsalpha NTDExpressionBacterialMutationPromoterlpp/lacUV5 (tandem promoter)Available sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pBAD24-fis
Plasmid#51557PurposeE. coli fis ORF cloned into pBAD24 expression vectorDepositorInsertFIS (fis E. coli)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJ211_WT_low
Plasmid#128848PurposeLow copy number plasmid for expression of E. coli flagellin from its natural promoterDepositorInsertFlagellin
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ211_WT_hi
Plasmid#128850PurposeHigh copy number plasmid for expression of E. coli flagellin from its natural promoterDepositorInsertFlagellin
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJB066
Plasmid#98913PurposeExpress FtsZ tagged with pAmCherry1 in E coliDepositorInsertFtsZ
UseTagspAmCherry1ExpressionBacterialMutationPromoterpBADAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJ291_WT_med
Plasmid#128856PurposeMedium copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoterDepositorInsertFlagellin
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEV_S
Plasmid#100266PurposepDSW204 carrying genes entABCDE, vibHF and a cos site, for serratiochelin and analogs production in E. coliDepositorInsertsUseSynthetic Biology; Expression, pbr322TagsExpressionBacterialMutationPromoterAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJ291_WT_low
Plasmid#128855PurposeLow copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoterDepositorInsertFlagellin
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ211_rha_WT_low
Plasmid#128851PurposeLow copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoterDepositorInsertFlagellin
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ211_rha_WT_med
Plasmid#128852PurposeMedium copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoterDepositorInsertFlagellin
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
Strep-TGT-His
Plasmid#206495PurposeExpresses E. Coli TGT with an N-terminal Strep Tag and a C-terminal His TagDepositorInsertE. coli tRNA Guanine Transglycosylase
UseTagsHis and StrepExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJ211_WT_med
Plasmid#128849PurposeMedium copy number plasmid for expression of E. coli flagellin from its natural promoterDepositorInsertFlagellin
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ211_rha_WT_hi
Plasmid#128853PurposeHigh copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoterDepositorInsertFlagellin
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
SSB-wt
Plasmid#197260Purposewild type E. coli SSB in pET21 expresses in BL21 under T7 control with lactose-amp resistanceDepositorInsertwt E. coli SSB
UseTagsnoneExpressionBacterialMutationPromoterT7Available sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACM4-ACA
Plasmid#49809PurposeEncodes fabD, accA, accB, accC, accD in pseudo-operon configuration.DepositorInsertsACP transacylase
Acetyl-CoA carboxylase
acetyl-CoA carboxylase
Acetyl-CoA carboxylase
Acetyl-CoA carboxylase
UseTagsExpressionBacterialMutationSilent site-directed mutagenesis to remove SalI s…PromoterT7Available sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCK314
Plasmid#110545PurposeModified (delta CRP-binding site) rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteriaDepositorInsertsModified PrhaBAD (CRP-binding sites removed)
yfp
rhaS
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEcRNAP6
Plasmid#128940PurposepET21a derivative encoding E. coli core RNA polymerase subunits: RpoA, B, C-ppx(His)10, ZDepositorInsertsUseTagsHisExpressionBacterialMutationPreScission protease site (GE Healthcare; LEVLFQG…PromoterT7Available sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
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His8:MBP-tev-Gly-eK-Flv
Plasmid#83383Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Trp-eK-Flv
Plasmid#83390Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Asp-eK-Flv
Plasmid#83389Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-His-eK-Flv
Plasmid#83388Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Lys-eK-Flv
Plasmid#83387Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only