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Showing: 151 - 200 of 12522 results

  1. pEU3-NII-HxHLICNot

    Plasmid
    #140183
    Purpose
    Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, 12xHis-tag and NotI restriction site
    Depositor
    Tamás Mészáros
    Article
    Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do
    Type
    Empty backbone
    Use
    Expression vector for a cell-free system
    Tags
    12xHis
    Expression
    Mutation
    Promoter
    T7
    Available since
    June 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    140183-plasmid-map-sequence-id-300400

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  8. His-ZZ-TEV-SNAPf DHC1_IC2C_LIC2_Tctex1_Robl1_LC8

    Plasmid
    #111903
    Purpose
    Full length human cytoplasmic dynein 1 complex, optimised for Sf9 expression. 8xHis-ZZ tag followed by TEV cleavage site and SNAPf tag on the N-terminus of dynein heavy chain 1.
    Depositor
    Andrew Carter
    Inserts
    His-ZZ-LTLT-SNAPf-DYNC1H1 (DYNC1H1 Human)
    DYNC1I2 (DYNC1I2 Human)
    DYNC1LI2 (DYNC1LI2 Human)
    DYNLT1 (DYNLT1 Human)
    DYNLRB1 (DYNLRB1 Human)
    DYNLL1 (DYNLL1 Human)
    Use
    Tags
    8xHis, SNAPf-tag, TEV site, and ZZ-tag
    Expression
    Insect
    Mutation
    Optimised for expression in Sf9 cells.
    Promoter
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111903-plasmid-map-sequence-id-215307

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  16. pCoHygro L16_shRNA of spliced Copia

    Plasmid
    #71389
    Purpose
    knock down expression of spliced Copia in Drosophila melanogaster
    Depositor
    Timothy Nilsen
    Insert
    Copia
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    U6
    Available since
    Nov. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    71389-plasmid-map-sequence-id-129772

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  31. lentiCRISPR - Amplicon, JAK2 sgRNA 1

    Plasmid
    #70679
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic JAK2 amplicon-targeting sgRNA element from U6 promoter. Lentiviral backbone
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against JAK2 amplicon found in AML-derived HEL cell line (JAK2 )
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  33. lentiCRISPR - Amplicon, JAK2 sgRNA 2

    Plasmid
    #70660
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic JAK2 amplicon-targeting sgRNA element from U6 promoter. Lentiviral backbone
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against JAK2 amplicon found in AML-derived HEL cell line (JAK2 )
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  38. gWiz_BM40-vAchT-Cytosolic-VL-hIgG1-CL

    Plasmid
    #218447
    Purpose
    mammalian expression plasmid for the light chain fragment of an anti-vAchT Fab domain binding to the cytosolic side of the transporter
    Depositor
    Axel Brunger
    Insert
    vAchT-Cytosolic-VL-hIgG1-CL
    Use
    Affinity Reagent/ Antibody
    Tags
    BM40
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    218447-plasmid-map-sequence-id-431100

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  39. pFHMSPN-avi-TEV-LIC V

    Plasmid
    #220885
    Purpose
    Donor vector to generate recombinant baculovirus by site-specific transposition in an E. coli host.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Baculovirus expresssion
    Tags
    Honeybee melittin signal followed by His, avi and…
    Expression
    Mutation
    Promoter
    Available since
    June 20, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    220885-plasmid-map-sequence-id-436698

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  41. pN23-LIC-FLAG-STREP

    Plasmid
    #182508
    Purpose
    pET based vector for protein expression in E.coli for targets fused with N-terminal Flag and STREP
    Depositor
    Renato Bruni
    Type
    Empty backbone
    Use
    Tags
    STREP-FLAG-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    182508-plasmid-map-sequence-id-361393

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  42. pNYCOMPS-C-Lic-His-FLAG

    Plasmid
    #182505
    Purpose
    pET based vector for protein expression in E.coli for targets fused with C-terminal Flag and His
    Depositor
    Renato Bruni
    Type
    Empty backbone
    Use
    Tags
    TEV-FLAG-10x His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    182505-plasmid-map-sequence-id-361391

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  43. pNYCOMPS-N-Lic-His-FLAG

    Plasmid
    #182506
    Purpose
    pET based vector for protein expression in E.coli for targets fused with N-terminal Flag and His
    Depositor
    Renato Bruni
    Type
    Empty backbone
    Use
    Tags
    10xHis-FLAG-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    182506-plasmid-map-sequence-id-358073

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  49. pEU3-NII-GLICNot-C-BIOT

    Plasmid
    #140186
    Purpose
    Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, GST-tag and biotinylation site, and NotI restriction site
    Depositor
    Tamás Mészáros
    Article
    Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do
    Type
    Empty backbone
    Use
    Expression vector for a cell-free system
    Tags
    Biotinylation and GST
    Expression
    Mutation
    Promoter
    T7
    Available since
    June 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    140186-plasmid-map-sequence-id-300441

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Showing: 151 - 200 of 12522 results