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Showing: 151 - 190 of 190 results

  12. pMO9075

    Plasmid
    #117481
    Purpose
    empty complementation vector for Desulfovibrio: contains promoter from Aph(3')-IIa but no RBS, contains pBG1 and pMB1 replicons, SpecR
    Depositor
    Judy Wall
    Article
    Keller et al Methods Enzymol. 2011;497:503-17. doi: 1
    Insert
    Aph(3')-IIa promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Aph(3')-IIa promoter
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    117481-plasmid-map-sequence-id-273023

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  17. D. vulgaris sp2 CRISPR/pACYCDuet-1

    Plasmid
    #81186
    Purpose
    Expresses CRISPR array containing 3 copies of Desulfovibrio vulgaris spacer 2 and flanking repeats in E. coli
    Depositor
    Jennifer Doudna
    Article
    Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:
    Insert
    Synthetic CRISPR array (3 copies of D. vulgaris spacer #2 flanked by repeats)
    Use
    CRISPR
    Tags
    None
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    81186-plasmid-map-sequence-id-162820

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  19. pAIP4_1UXZ

    Plasmid
    #234166
    Purpose
    Heterologous protein expression of cellulase B in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1UXZ
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234166-plasmid-map-sequence-id-464132

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  20. pY125 (pcDNA3.1-huBfCpf1)

    Plasmid
    #92301
    Purpose
    Expression of humanized BfCpf1
    Depositor
    Feng Zhang
    Insert
    huBfCpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    92301-plasmid-map-sequence-id-181503

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  21. pY124 (pcDNA3.1-huBsCpf1)

    Plasmid
    #92300
    Purpose
    Expression of humanized BsCpf1
    Depositor
    Feng Zhang
    Insert
    huBsCpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    92300-plasmid-map-sequence-id-181651

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  22. pY130 (pcDNA3.1-U6-huBsCpf1)

    Plasmid
    #92306
    Purpose
    Expression of humanized BsCpf1 and crRNA
    Depositor
    Feng Zhang
    Insert
    huBsCpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92306-plasmid-map-sequence-id-181649

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  24. pYPQ301

    Plasmid
    #205415
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Hs1Cas12a without promoter
    Depositor
    Yiping Qi
    Article
    Li et al Front Genome Ed. 2023 Oct 12;5:1251903.
    Insert
    Hs1Cas12a
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Nov. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    205415-plasmid-map-sequence-id-414100

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  25. 6xHis-MBP-huBfCas12a

    Plasmid
    #174672
    Purpose
    Expresses 6xHis-MBP tagged BfCas12a
    Depositor
    Piyush Jain
    Article
    Nguyen et al medRxiv 2021.07.21
    Insert
    huBfCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    174672-plasmid-map-sequence-id-345928

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  26. 6xHis-MBP-huBsCas12a

    Plasmid
    #174671
    Purpose
    Expresses 6xHis-MBP tagged BsCas12a
    Depositor
    Piyush Jain
    Article
    Nguyen et al medRxiv 2021.07.21
    Insert
    huBsCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV, NLS and NLS, 3xHA, T2A, EGFP, 6x…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    174671-plasmid-map-sequence-id-364005

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  27. 6xHis-MBP-huPxCas12a

    Plasmid
    #174675
    Purpose
    Expresses 6xHis-MBP tagged PxCas12a
    Depositor
    Piyush Jain
    Article
    Nguyen et al medRxiv 2021.07.21
    Insert
    huPxCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    174675-plasmid-map-sequence-id-346135

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  28. 6xHis-MBP-huPrCas12a

    Plasmid
    #174674
    Purpose
    Expresses 6xHis-MBP tagged PrCas12a
    Depositor
    Piyush Jain
    Article
    Nguyen et al medRxiv 2021.07.21
    Insert
    huPrCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    174674-plasmid-map-sequence-id-346134

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  32. pMO746

    Plasmid
    #117480
    Purpose
    upp in an artificial operon with npt from pMO9071 and AmpR-pUC ori from pCR4/TOPO, Pnpt-npt-upp;, KanR
    Depositor
    Judy Wall
    Article
    Parks et al Science. 2013 Mar 15;339(6125):1332-5. d
    Insert
    uracil phosphoribosyltransferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    117480-plasmid-map-sequence-id-273029

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  34. pMT1035

    Plasmid
    #228300
    Purpose
    Constitutive expression of LuxTA mutant with strong promoter
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    luxTA mutant
    Use
    Synthetic Biology
    Tags
    Nuclear localization signal-Three copies of VP16 …
    Expression
    Yeast
    Mutation
    S116Y, W201R
    Promoter
    KpGAPDH promoter
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228300-plasmid-map-sequence-id-457086

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  35. ePop

    Plasmid
    #50953
    Purpose
    Expresses E lysis protein that causes in subpopulation death when threshold cell density is reached, resulting in oscillations
    Depositor
    Lingchong You
    Article
    Marguet et al PLoS One. 2010 Jul 30;5(7):e11909. doi:
    Inserts
    E lysis protein
    LuxI
    LuxR
    Use
    Tags
    Expression
    Mutation
    Early stop codon introduced in mutated luxR resul…
    Promoter
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    50953-plasmid-map-sequence-id-454119

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  39. pYPQ287-RVR

    Plasmid
    #138124
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized BsCas12a with N512R, K518V and N522R mutations, without promoter
    Depositor
    Yiping Qi
    Article
    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:
    Insert
    BsCas12a RVR variant
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    N512R, K518V and N522R
    Promoter
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    138124-plasmid-map-sequence-id-272082

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Showing: 151 - 190 of 190 results