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Showing: 151 - 200 of 13385 results

  6. Anti-VAChT [N425/45R-2b]

    Plasmid
    #206672
    Purpose
    Mammalian Expression Plasmid of anti-VAChT vesicular acetylcholine transporter (Rat). Derived from hybridoma N425/45-2b.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    anti-VAChT vesicular acetylcholine transporter (Rattus norvegicus) recombinant Mouse monoclonal antibody (Slc18a3 Mouse)
    Expression
    Mammalian
    Promoter
    Dual CMV
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    206672-plasmid-map-sequence-id-413732

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  9. pOZ-FH-C Bach1 K141A/K142A

    Plasmid
    #73646
    Purpose
    Retroviral expression of Bach1 K141A/K142A mutant (mlh1 binding mutant)
    Depositor
    Sharon Cantor
    Insert
    Bach1 (BACH1 Human)
    Use
    Retroviral
    Tags
    FLAG and His
    Expression
    Mammalian
    Mutation
    K141A/K142A ( mlh1 binding mutant)
    Promoter
    unknown
    Available Since
    Jan. 7, 2019
    Availability
    Academic Institutions and Nonprofits only

  10. pCDNA3.1 Bach1 K141A/K142A

    Plasmid
    #73642
    Purpose
    Mammalian expression of Bach1 K141A/K142A mutant (mlh1 binding mutant)
    Depositor
    Sharon Cantor
    Insert
    Bach1 (BACH1 Human)
    Tags
    His and Myc
    Expression
    Mammalian
    Mutation
    K141A/K142A ( mlh1 binding mutant)
    Promoter
    CMV
    Available Since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only

  11. pcDNA3.1-nAChr-Alpha6-CFP

    Plasmid
    #50482
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Tags
    CFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the CFP open…
    Promoter
    CMV
    Available Since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. hSyn-FAS-DO-ReaChR-mCitrine-WPRE

    Plasmid
    #64035
    Purpose
    Expresses ReaChR under hSyn promoter. FAS-DO turns off expression in Cre-positive cells. FAS is compatible with other traditional Flex/DIO constructs
    Depositor
    Karel Svoboda
    Insert
    hSyn-FAS-DO-ReaChR-mCitrine-WPRE
    Use
    AAV
    Tags
    ReaChR tagged with mCitrine
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    May 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8 (ANV)

    Plasmid
    #71829
    Purpose
    Expression of mutant dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR and specific sgRNA for the human BACH2 promoter; for use as a control
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    BACH2-sgRNA8 (BACH2 Human, S. pyogenes, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    U6
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71829-plasmid-map-sequence-id-131024

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  20. pUt-mPF-BACH1 Cas9-Resistant_AmpR

    Plasmid
    #199221
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Positive-Feedback circuit (mPF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection. 
    Depositor
    Gabor Balazsi
    Article
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Insert
    rtTA-Advanced::P2A::EGFP::BACH1 (BACH1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    silient mutation at nucleic acid 177 C changed to…
    Promoter
    tight TRE promoter
    Available Since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199221-plasmid-map-sequence-id-396541

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  22. alpha3-nAChR shRNA (Alpha-3.29)

    Plasmid
    #86655
    Purpose
    expression of shRNA targeting CHRNA3
    Depositor
    Jaime Modiano
    Insert
    CHRNA3 (alpha3-nAChR) (CHRNA3 Human)
    Use
    RNAi
    Expression
    Mammalian
    Promoter
    H1
    Available Since
    June 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    86655-plasmid-map-sequence-id-180126

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  24. Ec (gamma-only)-pET(11a)(ACH–)

    Plasmid
    #241265
    Purpose
    Over-express E. coli Pol III subunit gamma-only in E. coli
    Depositor
    Michael O'Donnell
    Insert
    dnaXZ (gamma-only)
    Expression
    Bacterial
    Mutation
    No expression of tau
    Available Since
    Aug. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    241265-plasmid-map-sequence-id-482108

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  29. pUt-mNF-BACH1 Cas9-Resistant

    Plasmid
    #199218
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Negative-Feedback circuit (mNF-BACH1 Cas9- resistant). Use BlastR for positive selection and HSV-TK for negative selection. 
    Depositor
    Gabor Balazsi
    Article
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Insert
    hTetR::P2A::EGFP::BACH1 (BACH1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    silient mutation at nucleic acid 177 C changed to…
    Promoter
    CMV D2ir promoter
    Available Since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199218-plasmid-map-sequence-id-396470

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  30. alpha4-nAChR shRNA (Alpha-4.3)

    Plasmid
    #86652
    Purpose
    expression of shRNA targeting CHRNA4
    Depositor
    Jaime Modiano
    Insert
    CHRNA4 (alpha4-nAChR) (CHRNA4 Human)
    Use
    RNAi
    Expression
    Mammalian
    Promoter
    H1
    Available Since
    June 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    86652-plasmid-map-sequence-id-180128

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  31. Ec holA(Y316A)-FLAG-pET(11a)(ACH-)

    Plasmid
    #238346
    Purpose
    over-expresses C-terminal FLAG tagged E. coli delta (Y316A) in E. coli
    Depositor
    Michael O'Donnell
    Insert
    C-terminal FLAG tagged E. coli delta (Y316A) (holA E. coli)
    Tags
    3X FLAG
    Expression
    Bacterial
    Mutation
    Y316A
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238346-plasmid-map-sequence-id-472706

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  36. gWiz_BM40-vAchT-Cytosolic-VH-hIgG1-CH1-TS-HIS

    Plasmid
    #218446
    Purpose
    mammalian expression plasmid for the heavy chain fragment of an anti-vAchT Fab domain binding to the cytosolic side of the transporter
    Depositor
    Axel Brunger
    Insert
    vAchT-Cytosolic-Vh-hIgG1-CH6
    Use
    Affinity Reagent/ Antibody
    Tags
    BM40 and TwinStrep-6xHis
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    218446-plasmid-map-sequence-id-434499

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  44. Anti-VAChT [N425/45-rat IgG2a] - Chimeric

    Plasmid
    #227080
    Purpose
    Mammalian expression plasmid of anti-VAChT (Rattus norvegicus) rat IgG2a R-mAb. Derived from hybridoma N425/45.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-VAChT (Rattus norvegicus) recombinant mouse monoclonal antibody. (Slc18a3 Chimera: M. musculus (mouse)/R. norvegicus (rat))
    Use
    Affinity Reagent/ Antibody
    Expression
    Mammalian
    Promoter
    Dual CMV
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    227080-plasmid-map-sequence-id-450722

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  49. pAAV-SynI-CreOn-FlpOff-mNaChBacMUT-P2A-EGFP

    Plasmid
    #176281
    Purpose
    Viral vector for co-expression of non-functional NaChBac and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.
    Depositor
    Martin Riccomagno
    Article
    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    mNaChBacMUT-P2A-EGFP
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Mutation
    E191K
    Promoter
    human Synapsin I
    Available Since
    Jan. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    176281-plasmid-map-sequence-id-350685

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Showing: 151 - 200 of 13385 results