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Showing: 151 - 200 of 699 results

  1. pcDNA3.1-hRIPK1-Flag-delC

    Plasmid
    #112493
    Purpose
    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    deletion of C terminus (AA290-671 deleted)
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112493-plasmid-map-sequence-id-214530

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  2. pcDNA3.1-hRIPK1-Flag-delDD

    Plasmid
    #112494
    Purpose
    Mammalian expression of the indicated Flag- hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    deletion of DD domain (AA584-671 deleted)
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112494-plasmid-map-sequence-id-214528

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  3. pcDNA3-C17orf53_1-87-mCer

    Plasmid
    #211441
    Purpose
    mammalian expression of the N-terminal amino acids 1-87 of human C17orf53 C-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    C17orf53 (HROB Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Deletion of aa 88-646
    Promoter
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    211441-plasmid-map-sequence-id-419424

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  4. pcDNA3-C17orf53_1-87-mVen

    Plasmid
    #211442
    Purpose
    mammalian expression of the N-terminal amino acids 1-87 of human C17orf53 C-terminally fused to mVenus
    Depositor
    Helmut Pospiech
    Insert
    C17orf53 (HROB Human)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Deletion of aa 88-646
    Promoter
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211442-plasmid-map-sequence-id-419261

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  6. pcDNA3-Flag-RNF125 Ring Mutant

    Plasmid
    #122046
    Purpose
    express RNF125 RM
    Depositor
    Ze'ev Ronai
    Insert
    RNF125 RM (RNF125 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Ring mutant C37A, C40A
    Promoter
    Available since
    June 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    122046-plasmid-map-sequence-id-242459

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  7. pcDNA3-sACE2v1

    Plasmid
    #145148
    Purpose
    Mammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 1
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    145148-plasmid-map-sequence-id-292584

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  11. SSH-pcDNA3.1-α-COPI-WD40-Arg13Ala

    Plasmid
    #208172
    Purpose
    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 Arg13Ala mutant (residues 1-327)
    Depositor
    Syed Saif Hasan
    Article
    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:
    Insert
    N-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Arg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys)
    Use
    Tags
    Strep-tag
    Expression
    Mammalian
    Mutation
    Arg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196…
    Promoter
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    208172-plasmid-map-sequence-id-432648

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  12. SSH-pcDNA3.1-α-COPI-WD40-Arg300Ala

    Plasmid
    #208173
    Purpose
    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg300Ala (residues 1-327)
    Depositor
    Syed Saif Hasan
    Article
    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:
    Insert
    N-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys,Arg300Ala)
    Use
    Tags
    Strep-tag
    Expression
    Mammalian
    Mutation
    Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe19…
    Promoter
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    208173-plasmid-map-sequence-id-436198

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  13. SSH-pcDNA3.1-α-COPI-WD40-Lys15Ala

    Plasmid
    #208174
    Purpose
    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Lys15Ala (residues 1-327)
    Depositor
    Syed Saif Hasan
    Article
    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:
    Insert
    N-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Lys15Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys)
    Use
    Tags
    Strep-tag
    Expression
    Mammalian
    Mutation
    Lys15Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196…
    Promoter
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    208174-plasmid-map-sequence-id-436200

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  15. pcDNA3-C17orf53_1-241-mCer

    Plasmid
    #211443
    Purpose
    mammalian expression of the N-terminal amino acids 1-241 of human C17orf53 C-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    C17orf53 (HROB Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Deletion of aa 242-646
    Promoter
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    211443-plasmid-map-sequence-id-419423

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  16. pcDNA3-C17orf53_1-241-mVen

    Plasmid
    #211444
    Purpose
    mammalian expression of the N-terminal amino acids 1-241 of human C17orf53 C-terminally fused to mVenus
    Depositor
    Helmut Pospiech
    Insert
    C17orf53 (HROB Human)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Deletion of aa 242-646
    Promoter
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211444-plasmid-map-sequence-id-419266

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  21. pcDNA3-CTE-Luc

    Plasmid
    #60881
    Purpose
    Reporter construct to study unspliced RNA export. Coding sequence of luciferase gene was sandwiched between 5' and 3' splice site sequences originating from the HIV gag/pol gene followed by CTE
    Depositor
    Vinay Nandicoori
    Article
    Rajanala et al PLoS One. 2012;7(1):e29921. doi: 10.1371
    Insert
    Luc-CTE
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    60881-plasmid-map-sequence-id-98231

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  33. pcDNA3.3_CoV1_D28

    Plasmid
    #170447
    Purpose
    Express SARS-CoV-1 WT spike protein with a 28aa deletion on the C-terminal tail. Used for SARS1 pseudotyped virus production.
    Depositor
    David Nemazee
    Article
    Rogers et al Science. 2020 Aug 21;369(6506):956-963.
    Insert
    SARS-CoV-1 spike D28 (S SARS-CoV-1)
    Use
    Tags
    NA
    Expression
    Mammalian
    Mutation
    last 28aa deletion in c-terminal tail
    Promoter
    CMV
    Available since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    170447-plasmid-map-sequence-id-333040

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  38. pcDNA3-Nrxn1beta AS4(-)-Fc

    Plasmid
    #59313
    Purpose
    Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]
    Depositor
    Peter Scheiffele, Tito Serafini
    Article
    Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.
    Insert
    Nrxn1beta AS4(-)-Fc fusion (Nrxn1 Mouse)
    Use
    Tags
    Fc (from human IgG)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    June 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    59313-plasmid-map-sequence-id-107095

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  39. pcDNA3-Nrxn1beta AS4(+)-Fc

    Plasmid
    #59314
    Purpose
    Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]
    Depositor
    Peter Scheiffele, Tito Serafini
    Article
    Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.
    Insert
    Nrxn1beta AS4(+)-Fc fusion (Nrxn1 Mouse)
    Use
    Tags
    Fc (from human IgG)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    June 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    59314-plasmid-map-sequence-id-107094

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  42. pcDNA3.1-H2-18 Heavy chain-SpyTag003

    Plasmid
    #216307
    Purpose
    Expresses anti-HER2 H2-18 Heavy chain-SpyTag003 in mammalian cells. To be paired with H2-18 light chain to form the anti-HER2 H2-18 Fab
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    H2-18 Heavy chain-SpyTag003
    Use
    Affinity Reagent/ Antibody
    Tags
    His6 tag, Signal peptide, and SpyTag003
    Expression
    Mammalian
    Mutation
    Promoter
    T7 promoter
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216307-plasmid-map-sequence-id-433370

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  44. pcDNA3.1-MF3958 Heavy chain-SpyTag003

    Plasmid
    #216303
    Purpose
    Expresses anti-HER2 MF3958 Heavy chain-SpyTag003 in mammalian cells. To be paired with MF3958 light chain to form the anti-HER2 MF3958 Fab
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    MF3958 Heavy chain-SpyTag003
    Use
    Affinity Reagent/ Antibody
    Tags
    His6 tag, Signal peptide, and SpyTag003
    Expression
    Mammalian
    Mutation
    Promoter
    T7 promoter
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216303-plasmid-map-sequence-id-433368

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  45. pcDNA3.1-39S Heavy chain-SpyTag003

    Plasmid
    #216296
    Purpose
    Expresses anti-HER2 39S Heavy chain-SpyTag003 in mammalian cells. To be paired with 39S light chain to form the anti-HER2 39S Fab
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    39S Heavy chain-SpyTag003
    Use
    Affinity Reagent/ Antibody
    Tags
    His6 tag, Signal peptide, and SpyTag003
    Expression
    Mammalian
    Mutation
    Promoter
    T7 promoter
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216296-plasmid-map-sequence-id-433365

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  46. pcDNA3.1-CMV-LMO2 (GLuc-VChR1-EYFP)

    Plasmid
    #72889
    Purpose
    fusion protein of Gaussia luciferase, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Article
    Berglund et al PLoS One. 2013;8(3):e59759. doi: 10.1371
    Inserts
    Gaussia luciferase, Volvox channelrhodopsin 1, EYFP
    Volvox channelrhodopsin 1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    72889-plasmid-map-sequence-id-252489

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Showing: 151 - 200 of 699 results