-
Plasmid#227224PurposeExpresses human USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationE345KPromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
V485G FL USP7
Plasmid#227222PurposeExpresses human USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationV485GPromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPbHiT_3xmyc_hsp70UTR_hdhfr/yfcu
Plasmid#216421PurposeEmpty backbone to express gene specific gRNA and carry the homology repair template for CRISPR editing of Plasmodium berghei using the PbHiT system.DepositorTypeEmpty backboneUseCRISPR; Expression in plasmodium bergheiTags3xmycExpressionBacterialMutationPromoterAvailable sinceApril 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
PRE-Hsp70BbCas9_1.0
Plasmid#190795PurposePRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements. Step 1 in cloning processDepositorInsertPRE-Hsp70Bb-Cas9-T2A-eGFP
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDmHsp70b-TACT
Plasmid#165780PurposeHSP70B promoter for fusion with bacterial promotersDepositorInsertHsp70b-TACT
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis-7D12Asp73TAG
Plasmid#125266PurposeExpresses 7D12 anti-EGFR nanobodies with UAG codon at Asp73 for synthetic amino acid in combination with pEVOL-pAzFDepositorInsert7D12 Asp73TAG
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLIB-RSP7
Plasmid#176696PurposeExpression of Chlamydomonas reinhardtii RSP7 recombinant protein in insect cellsDepositorInsertRSP7
UseTagsExpressionInsectMutationNonePromoterpolyhedrinAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_WT USP7
Plasmid#131242PurposeMammalian expression of N-terminally Myc-tagged USP7DepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
UseTagsMycExpressionMammalianMutationPromoterCMV enhancer + CMV promoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Casp7-Flag
Plasmid#11815DepositorInsertCaspase 7 (CASP7 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
pQFlag-USP7 WT puroR
Plasmid#46751PurposeRetroviral vector that expresses wild type Flag-tagged human USP7DepositorInsertUSP7 (USP7 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
hsp70: mKate2-DN Kif5a
Plasmid#105972Purposeheat shock inducible transgenesisDepositorInsertKif5a (kif5aa Zebrafish)
UseTagsmKate2ExpressionBacterialMutationtruncation of aa416-1033PromoterAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_C223S USP7
Plasmid#131243PurposeMammalian expression of catalytically inactive Myc-tagged USP7DepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
UseTagsMycExpressionMammalianMutationContains a point mutation that converts USP7 cyst…PromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
hsp70l: hAPOB48-mCherry.
Plasmid#140639PurposeHeat shock promoter driven expression of human APOB48-mCherry. The construct has tol2 elements, and is designed to use in zebrafish.DepositorUseZebrafish expressionTagsmCherryExpressionMutationAPOB48: amino acid 1-2180Promoterhsp70lAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQFlag-USP7 CS puroR
Plasmid#46752PurposeRetroviral vector that expresses catalytically inactive form of Flag-tagged human USP7DepositorInsertUSP7 (USP7 Human)
UseRetroviralTagsFlagExpressionMammalianMutationC223S--catalytically inactivePromoterCMVAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-Hsp70-Cas9
Plasmid#46294PurposeA codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.DepositorInsertcodon optimized Cas9
UseCRISPRTags3x FlagExpressionInsectMutationSilent change from C to A at bp 834 of insertPromoterHsp70Available sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDFDuet-nsp7-nsp8
Plasmid#159092PurposeCoexpression construct of Nsp7 with an N-terminal His-tag and Nsp8DepositorInsertsNSP7
NSP8
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP hsp70 K71E
Plasmid#15216DepositorInserthsp70 K71E (HSPA1A Human)
UseTagsEGFPExpressionMammalianMutationK71E, ATPase-deficient mutant.PromoterAvailable sinceJune 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pQHA-USP7 WT puroR
Plasmid#46753PurposeRetroviral vector that expresses wild type HA-tagged human USP7DepositorInsertUSP7 (USP7 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQHA-USP7 CS puroR
Plasmid#46754PurposeRetroviral vector that expresses catalytically inactive form of HA-tagged human USP7DepositorInsertUSP7 (USP7 Human)
UseRetroviralTagsHAExpressionMammalianMutationC223S--catalytically inactivePromoterCMVAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET23b-Casp7-His
Plasmid#11825DepositorInsertCaspase 7 (CASP7 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only