We narrowed to 936 results for: trna

Showing: 61 - 80 of 936 results

Results per page:

Sort results by:

pX458-tRNA-SpCas9

Plasmid

#195183

Purpose

Vector for transient Cas9 and EGFP expression. Small tRNA promoter for sgRNA cloning by GoldenGate.

Depositor

Stephanie Roessler

Insert

Cas9

Use

CRISPR

Promoter

tRNA-Gln

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRANS-Pho2-1/2-2-tRNA

Plasmid

#161762

Purpose

pTRANS T-DNA vector with nptII selection and 35S:Cas9 with 6x tRNA-spliced gRNAs and rolD:TREX2 targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pTRANS_220 - #91113)

Depositor

Shaun Curtin

Article

Miller et al G3 (Bethesda). 2022 May 30;12(6). pii: 6

Insert

Cas9 and gRNAs

Expression

Plant

Promoter

CmYLCV Promoter

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_B-Pho2-1/2-2-tRNA

Plasmid

#161761

Purpose

MODULE B tRNA vector with 6x gRNAs targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pMOD_B2303 - #91068)

Depositor

Shaun Curtin

Article

Miller et al G3 (Bethesda). 2022 May 30;12(6). pii: 6

Insert

gRNAs

Expression

Bacterial

Promoter

CmYLCV Promoter

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-tRNA-Gly

Plasmid

#174317

Purpose

For in vitro transcription of mouse tRNAs

Depositor

Bruce Beutler

Article

Yue et al Science. 2021 May 14;372(6543). pii: 372

Insert

tRNA-gly

Expression

Bacterial

Promoter

T7

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_KARS_tRNA-anti-codon

Plasmid

#109865

Purpose

Protein expression and purification of KARS_tRNA-anti-codon

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

KARS_tRNA-anti-codon (KARS1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-10X-tRNA

Plasmid

#70123

Purpose

Gateway DONR vector with cassette for expression of 10 intronless S. cerevisiae tRNAs

Depositor

Jay Hesselberth

Article

Cherry et al RNA. 2018 Mar;24(3):313-323. doi: 10.126

Insert

10X-tRNA block

Promoter

None – SUP4 pol III promoter provided by insert

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only
FASTHDR-tRNApromo-sgRNA-espCas91_1

Plasmid

#167210

Purpose

Plasmid for easy cloning of a sgRNA sequence. The Plasmid contain a tRNA promoter to facilitate the expression of any sgRNA sequence and also expresses eSpCas9(1.1)

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

Plasmid

#182287

Purpose

Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells

Depositor

Ivana Nikić-Spiegel

Article

Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

Inserts

codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
PylT

Tags

nuclear export signal (NES)

Expression

Mammalian

Mutation

Y306A/Y384F (AF) double mutant of Methanosarcina …

Promoter

CMV and U6

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSMT-MjtRNA-GFP(47TAG)

Plasmid

#193251

Purpose

Expresses the M. jannaschii tRNATyr (MjtRNA) and GFP(47TAG) (aka GFP151TAG)

Depositor

Peter Schultz, Jessica Seeliger

Article

Touchette et al ACS Infect Dis. 2017 May 12;3(5):336-348

Inserts

GFP(47TAG)
MjtRNA

Expression

Bacterial

Mutation

Codon for Tyr47 replaced with amber stop codon TA…

Promoter

hsp60 and metU

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMB1_MmPylRS_MmtRNA-Pyl-opt(UGA)

Plasmid

#174515

Purpose

Recoded single aaRS/tRNA plasmid with MmPylRS and MmtRNA-Pyl-opt with the ASL from the serT gene (UGA anticodon)

Depositor

Jason W Chin

Article

Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

Inserts

Mm PylS
MmtRNA-Pyl-opt(UGA)

Expression

Bacterial

Mutation

ASL from the serT gene (UGA anticodon) and recoded

Promoter

GlnS and lpp

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-tRNA scaffolded SA (tRSA)

Plasmid

#32200

Depositor

Ian Macara

Article

Iioka et al Nucleic Acids Res. 2011 Apr;39(8):e53. E

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK-N. tabacum trnI/trnA

Plasmid

#123579

Purpose

MoChlo kit

Depositor

Scott Lenaghan, C. Neal Stewart

Article

Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

Insert

N. tabacum trnI/trnA integration site

Use

Chloroplast transformation vector

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTZ tRNA_gRNA empty scaffold

Plasmid

#188450

Purpose

Expression of gRNAs using a human tRNA promoter

Depositor

Ajit Chande

Article

Mishra et al Mol Ther Nucleic Acids. 2022 Mar 8;28:20

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

tRNA glutamine

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
tRNA-gRNA position [n] (GB1207)

Plasmid

#75408

Purpose

tRNA and scaffold for the assembly of GBoligomers for the last position (positon [n]) of a polycistronic tRNA-gRNA (2- and 3-part multiplexing)

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

tRNA-gRNA position [n]

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK-S. tuberosum trnI/trnA

Plasmid

#123583

Purpose

MoChlo kit

Depositor

Scott Lenaghan, C. Neal Stewart

Article

Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

Insert

S. tuberosum trnI/trnA integration site

Use

Chloroplast transformation vector

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGA1-EF1α-sfGFP150TAG-4xtRNA

Plasmid

#251517

Purpose

Expression of sfGFP150TAG using the Methanomethylophilus alvus (Ma) PylRS/tRNA system for site-specific incorporation of noncanonical amino acids at TAG codons in HEK293 cells

Depositor

Ryan Mehl

Article

Augustin et al J Am Chem Soc. 2026 Feb 12. doi: 10.1021

Insert

sfGFP150TAG

Tags

His6 and V5

Expression

Mammalian

Mutation

150 TAG (amino acid 150 mutated to stop codon)

Promoter

EF1alpha

Available Since

March 12, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
EKv376(gRNAscaffold-tRNA for cloning)

Plasmid

#248333

Purpose

Plasmid contains gRNA scaffold-tRNA and is used to amplify fragments for Golden Gate assembly

Depositor

Elena Kozgunova

Article

Kozgunova et al bioRxiv 2025.07.29.667574

Insert

gRNAscaffold-tRNA

Use

CRISPR

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAVS1-P-6XPyltRNA-dCas9K1151TAG-IRESPylRS

Plasmid

#248695

Purpose

Expression dead CRISPR/Cas9 with unnatural amino acid incorporated at K1151 residue

Depositor

Shuibing Chen

Article

Zhu et al Proc Natl Acad Sci U S A. 2025 Oct 7;122

Insert

dead CRISPR/Cas9 K1151TAG, MbPylRS

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBR-sfGFP N149TAG-MjtRNA

Plasmid

#242001

Purpose

Expresses MjtRNA and contains sfGFP gene with amber codon at 149 position.

Depositor

Wei Niu

Article

He et al ACS Synth Biol. 2022 Nov 18;11(11):3724-

Insert

sfGFPN149TAG

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
UAS-4x(tRNA::axed{sgRNA})

Plasmid

#187883

Purpose

Gal4/UAS sgRNA expression targeting axed

Depositor

Lukas Neukomm

Article

Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

Insert

4 sgRNAs targeting axed

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge