4,143 results

1. pSIV-D3psi/delta env/delta Vif/delta Vpr

   Plasmid

   #132928

   Purpose

   Production of SIV-VLPs containing Vpx protein

   Depositor

   Jeremy Luban

   Article

   Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

   Insert

   Gag, pol, and vpx
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. myc-hULK1

   Plasmid

   #31961

   Depositor

   Do-Hyung Kim

   Article

   Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

   Insert

   ULK1 (ULK1 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, SP6

   Available since

   Sept. 1, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. TETv4

   Plasmid

   #167983

   Purpose

   Expresses TETv4 (TET1CD-XTEN80-dCas9-3xNLS-P2A-BFP) downstream of the CAG promoter

   Depositor

   Luke Gilbert

   Article

   Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

   Insert

   TETv4 (TET1CD-XTEN80-dCas9)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   3xNLS, TET1 catalytic domain, and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-hPIKfyve

   Plasmid

   #121148

   Purpose

   Expresses GFP-tagged human PIKfyve in mammalian cells.

   Depositor

   Geert van den Bogaart

   Article

   Baranov et al iScience. 2019 Jan 25;11:160-177. doi: 1

   Insert

   1-phosphatidylinositol 3-phosphate 5-kinase isoform 2 (PIKFYVE Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. gag/pol

   Plasmid

   #14887

   Depositor

   Tannishtha Reya

   Article

   Reya et al Nature. 2003 May 22. 423(6938):409-14.

   Insert

   gag / pol
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGTR

   Plasmid

   #63143

   Purpose

   PCR template to construct polycistronic tRNA-gRNA

   Depositor

   Yinong Yang

   Article

   Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

   Insert

   gRNA-tRNA fusion
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. Human Beta-catenin GFP

   Plasmid

   #71367

   Purpose

   mammalian expression of human Beta-catenin GFP

   Depositor

   Alpha Yap

   Article

   Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

   Insert

   Beta-catenin (CTNNB1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP-E-Syt1

   Plasmid

   #66830

   Purpose

   Mammalian Expression, Bacterial Expression

   Depositor

   Pietro De Camilli

   Article

   Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

   Insert

   E-Syt1 (ESYT1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZac2.1 gfaABC1D-lck-GCaMP6f

   Plasmid

   #52924

   Purpose

   Fluorescent reporter for calcium signaling

   Depositor

   Baljit Khakh

   Has Service

   AAV5

   Insert

   lck-GCaMP6f
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmal_c5x_RNAse_Inhib

    Plasmid

    #153314

    Purpose

    Bacterial expression of an RNase inhibitor

    Depositor

    Drew Endy, Philippa Marrack

    Insert

    RNAse Inhibitor
    

    Use

    Tags

    Maltose Binding Protein

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. sfGFP-C1

    Plasmid

    #54579

    Purpose

    Localization: C1 Cloning Vector, Excitation: 485, Emission: 507

    Depositor

    Geoffrey Waldo, Michael Davidson

    Article

    Pédelacq et al Nat Biotechnol. 2006 Jan;24(1):79-88. do

    Type

    Empty backbone

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pcDNA3.1/hChR2(H134R)-EYFP

    Plasmid

    #20940

    Purpose

    Mammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    CMV

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. myc-BioID2-MCS

    Plasmid

    #74223

    Purpose

    To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tag

    Depositor

    Kyle Roux

    Article

    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    Myc and humanized A. aeolicus BioID2 with R40G

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-FLEX-ArchT-tdTomato

    Plasmid

    #28305

    Purpose

    Cre-dependent viral expression of ArchT-tdTomato for neuronal inhibition

    Depositor

    Edward Boyden

    Article

    Han et al Front Syst Neurosci. 2011;5:18. Epub 201

    Has Service

    AAV5

    Insert

    ArchT-tdTomato
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available since

    Aug. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)

    Plasmid

    #107941

    Purpose

    Mammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutions

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized enAsCas12a (E174R/S542R/K548R)
    

    Use

    Tags

    3x HA and NLS (nucleoplasmin)

    Expression

    Mammalian

    Mutation

    E174R, S542R and K548R

    Promoter

    CAG

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMVR8.74

    Plasmid

    #22036

    Purpose

    2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)

    Depositor

    Didier Trono

    Insert

    gag pol tat rev
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. mEmerald-Sec61b-C1

    Plasmid

    #90992

    Purpose

    Expresses mEmerald-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

    Insert

    mEmerald-Sec61b (SEC61B Synthetic, Human)
    

    Use

    Tags

    mEmerald (green fluorescence)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRIS-PITChv2-Puro-dTAG (BRD4)

    Plasmid

    #91793

    Purpose

    PITCh dTAG donor vector for Puro-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

    Depositor

    James Bradner, Behnam Nabet

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Insert

    Puro-P2A-2xHA-FKBP_F36V
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    Promotorless

    Available since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCI HA NEDD4

    Plasmid

    #27002

    Depositor

    Joan Massague

    Article

    Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

    Insert

    NEDD4 (NEDD4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT7-hMLH1dn for IVT

    Plasmid

    #178114

    Purpose

    Template for in vitro transcription of human MLH1dn

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    human MLH1dn (codon optimized) (MLH1 Human)
    

    Use

    Template for in vitro transcription

    Tags

    Expression

    Mutation

    Detailed in manuscript

    Promoter

    T7 (inactivated)

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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