4,168 results

1. pXR004: CasRx pre-gRNA cloning backbone

   Plasmid

   #109054

   Purpose

   hU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRs

   Depositor

   Patrick Hsu

   Article

   Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Slug_pGL2

   Plasmid

   #31695

   Depositor

   Paul Wade

   Article

   Fujita et al Cell. 2003 Apr 18. 113(2):207-19.

   Insert

   Slug (SNAI2 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EF1a-FLEX-GTB

   Plasmid

   #26197

   Depositor

   Edward Callaway

   Article

   Haubensak et al Nature. 2010 Nov 11. 468(7321):270-6.

   Insert

   Enhanced green fluorescent protein, TVA, rabies B19 glycoprotein
   

   Use

   AAV and Cre/Lox; Adeno-associated virus

   Mutation

   TVA, rabies glycoprotein are mammalian codon opti…

   Available Since

   Nov. 11, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFP-Rab7A T22N

   Plasmid

   #28048

   Depositor

   Qing Zhong

   Article

   Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

   Insert

   Rab7A (RAB7A Human)
   

   Tags

   2xHA and EGFP

   Expression

   Mammalian

   Mutation

   T22N

   Available Since

   May 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTARA

   Plasmid

   #31491

   Depositor

   Kathleen Matthews

   Article

   Wycuff et al Anal Biochem. 2000 Jan 1. 277(1):67-73.

   Insert

   RNA polymerase
   

   Expression

   Bacterial

   Mutation

   N823D

   Available Since

   June 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3XFlagNICD2

   Plasmid

   #20184

   Depositor

   Raphael Kopan

   Article

   Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

   Insert

   Notch 2 intracellular domain (Notch2 Mouse)
   

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   Contains murine Notch 2 Intracellular Domain (Met…

   Promoter

   CMV

   Available Since

   Jan. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pME pHluorin2

   Plasmid

   #73794

   Purpose

   ratiometric pHluorin2 sequence in a middle entry clone

   Depositor

   David Raible

   Article

   Stawicki et al Dis Model Mech. 2014 Jul;7(7):847-56. do

   Insert

   ratiometric pHluorin2
   

   Use

   Middle entry clone

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. Flag-hPIASx beta

   Plasmid

   #15210

   Depositor

   Ke Shuai

   Article

   Arora et al J Biol Chem. 2003 Jun 13. 278(24):21327-

   Insert

   PIASx beta (PIAS2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Feb. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLCAR-CD19-CD3z

   Plasmid

   #135993

   Purpose

   Modular CD3z alone CAR backbone, For Transient Expression or Lentiviral Production

   Depositor

   Scott McComb

   Article

   Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

   Insert

   FMC63-3z-P2A-EGFP (CD247, CD28 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a-short

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Flag-hPIASy

    Plasmid

    #15208

    Depositor

    Ke Shuai

    Article

    Liu et al Proc Natl Acad Sci U S A. 2001 Mar 13. 9

    Insert

    PIASy (PIAS4 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-ChrimsonR-tdT

    Plasmid

    #59169

    Purpose

    Expression vector for ChrimsonR-tdTomato

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-tdTomato
    

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    K176R

    Promoter

    CAG

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. Flag-hPIASx alpha

    Plasmid

    #15209

    Depositor

    Ke Shuai

    Article

    Arora et al J Biol Chem. 2003 Jun 13. 278(24):21327-

    Insert

    PIASx alpha (PIAS2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

13. HA-HIF1alpha-pcDNA3

    Plasmid

    #18949

    Depositor

    William Kaelin

    Article

    Kondo et al Cancer Cell. 2002 Apr . 1(3):237-46.

    Insert

    Hypoxia inducible factor 1 alpha (HIF1A Human)
    

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt cDNA

    Available Since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH-CMV

    Plasmid

    #72265

    Purpose

    Express gene of interest under CMV promoter

    Depositor

    Kazuhiro Oka

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 25, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. Flag-mPIAS3

    Plasmid

    #15207

    Depositor

    Ke Shuai

    Article

    Chung et al Science. 1997 Dec 5. 278(5344):1803-5.

    Insert

    PIAS3 (Pias3 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    V580I

    Available Since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC1-HyPer-Red

    Plasmid

    #48249

    Purpose

    Genetically encoded hydrogen peroxide indicator with red fluorescence.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

    Insert

    HYPER-RED
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPomyces-2

    Plasmid

    #61737

    Purpose

    Streptomyces expression of codon-optimized Cas9 and custom gRNA

    Depositor

    Huimin Zhao

    Article

    Cobb et al ACS Synth Biol. 2014 Dec 8.

    Inserts

    sSpCas9
    gRNA cassette
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    BbsI-flanked lacZ cassette inserted in place of s…

    Promoter

    gapdhp(EL) and rpsL(XC)-BbsI

    Available Since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL3-NFAT luciferase

    Plasmid

    #17870

    Purpose

    3x NFAT binding sequence upstream of a luciferase reporter; can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissue

    Depositor

    Jerry Crabtree

    Article

    Clipstone et al Nature. 1992 Jun 25. 357(6380):695-7.

    Insert

    3x NFAT binding sequence (IL2 )
    

    Use

    Luciferase

    Tags

    IL2 promoter and Luciferase

    Expression

    Mammalian

    Promoter

    IL-2 promoter

    Available Since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. ER-GCaMP6-150

    Plasmid

    #86918

    Purpose

    Fluorescent reporter for ER calcium signaling

    Depositor

    Timothy Ryan

    Article

    de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

    Insert

    ER-GCaMP6-150
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

    Promoter

    CAMKII

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. CMV-Mito4x-GCaMP6f

    Plasmid

    #127870

    Purpose

    Fluorescent reporter for mitochondrial matrix Ca2+

    Depositor

    Timothy Ryan

    Article

    Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

    Insert

    4 repeats of COX8 signal peptide plus GCaMP6f
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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