19,660 results

1. pBabe puro c-met WT

   Plasmid

   #17493

   Depositor

   Joan Brugge

   Article

   Wrobel et al J Cell Biol. 2004 Apr 26. 165(2):263-73.

   Insert

   c-Met (Met Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pORANGE GFP-Grin2b KI

   Plasmid

   #131487

   Purpose

   Endogenous tagging of GluN2b: N-terminal (amino acid position: S34)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Grin2b Rat)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.CMV.ffLuciferase.SV40

   Plasmid

   #105532

   Purpose

   AAV expression of firefly luciferase from CMV promoter

   Depositor

   James M. Wilson

   Has Service

   AAV8

   Insert

   Luciferase
   

   Use

   AAV and Luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-N1-human lyn–GFP

   Plasmid

   #35958

   Purpose

   Expresses human lyn protein codon optimized for zebrafish expression.

   Depositor

   Anna Huttenlocher

   Article

   Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

   Insert

   LYN (LYN Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   cmv

   Available Since

   April 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. Nsp4-EGFP

   Plasmid

   #165109

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   Nsp4 (ORF1ab codon optimized SARS-COV-2)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin

   Plasmid

   #134661

   Purpose

   Expresses EGFP-tagged synaptic vesicle protein

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   vesicle-associated membrane protein 2 (VAMP2 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBabe B-Raf V600E

   Plasmid

   #17544

   Depositor

   Channing Der

   Article

   Hao et al Mol Cancer Ther. 2007 Aug . 6(8):2220-9.

   Insert

   B-Raf (BRAF Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   V600E

   Available Since

   March 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. CROPseq-multi-EBFP2-NLS (pRW1447)

   Plasmid

   #216219

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi construct with nuclear-localized EBFP2 expression

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCALNL-GFP

   Plasmid

   #13770

   Purpose

   Cre recombinase-dependent expression of GFP, i.e. GFP is only expressed in the presence of Cre due to a transcriptional stop in between the loxP sites.

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   EGFP
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNIC-CTHF

    Plasmid

    #26105

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Opher Gileadi

    Article

    Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

    Type

    Empty backbone

    Tags

    TEV - His6 - Flag

    Expression

    Bacterial

    Available Since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pICE-FLAG-NAT10-siR-WT

    Plasmid

    #59365

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of wild-type human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Steve Jackson

    Article

    Larrieu et al Science. 2014 May 2;344(6183):527-32. do

    Insert

    human NAT10 (NAT10 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Mutations to render cDNA resistant to a siRNA

    Promoter

    CMV-tet

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

    Plasmid

    #42229

    Purpose

    This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS-Flag-hsDicer

    Plasmid

    #41584

    Depositor

    Phil Sharp

    Article

    Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

    Insert

    hsDicer (DICER1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Jan. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. EF1a-mEmerald-CAPRIN1

    Plasmid

    #164218

    Purpose

    Expresses mEmerald tagged CAPRIN1 under EF-1a promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    CAPRIN1 (CAPRIN1 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Promoter

    EF-1a

    Available Since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-EGFP-Cdc42(T17N)

    Plasmid

    #12601

    Depositor

    Klaus Hahn

    Article

    Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

    Insert

    EGFP-Cdc42 (T17N) (CDC42 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    T17N

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQCXIP-EGFP-F

    Plasmid

    #73014

    Purpose

    Retroviral expression of EGFP-F

    Depositor

    Michael Grusch

    Insert

    EGFP-F
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMK293 (mAID-mCherry2-Hygro)

    Plasmid

    #72831

    Purpose

    C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-mCherry2-Hygro
    

    Use

    CRISPR

    Tags

    mAID-mCherry2

    Expression

    Mammalian

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHT-ads

    Plasmid

    #47382

    Purpose

    Expresses ads under Pgrac promoter (inducible by IPTG) in bacteria

    Depositor

    Heng-Phon Too

    Article

    Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

    Insert

    ads
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    Pgrac

    Available Since

    Oct. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. FUW-tetO-EGFP

    Plasmid

    #84041

    Purpose

    3rd generation lentiviral transfer plasmid. Expresses EGFP and puromycin resistance in mammalian cells under the control of a doxycycline-inducible promoter

    Depositor

    Stefano Piccolo

    Article

    Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

    Insert

    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE-CMV

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. YFP NLS Beta-Actin S14C

    Plasmid

    #60614

    Purpose

    Encodes YFP and NLS tagged beta-actin with the S14C polymerization mutation

    Depositor

    Primal de Lanerolle

    Article

    Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

    Insert

    Beta-actin (ACTB Human)
    

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Mutation

    S14C

    Available Since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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