We narrowed to 12,722 results for: CAR

1. pAAV-GFAP-eGFP-shLacZ

   Plasmid

   #223222

   Purpose

   mir30 based shRNA strategy, control shRNA

   Depositor

   Li-Huei Tsai

   Article

   Murdock et al Nature. 2024 Mar;627(8002):149-156. doi:

   Insert

   shLacZ
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCGB3A2_HL-P2A-TagBFP-PGK-NeoR-SCGB3A2_HR

   Plasmid

   #126697

   Purpose

   donor vector for targeting a 2A peptide followed by blue fluorescent reporter to the human SCGB3A2 locus at the end of the endogenous coding sequence

   Depositor

   Darrell Kotton

   Article

   McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

   Insert

   TagBFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHDM-SARS-CoV-2

   Plasmid

   #164433

   Purpose

   Encodes SARS-CoV-2 Spike Protein for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Insert

   SARS-CoV-2 Spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-SCGB3A2_gRNA1-SpCas9-T2A-GFP

   Plasmid

   #126698

   Purpose

   to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human SCGB3A2 locus at the end of the endogenous coding sequence

   Depositor

   Darrell Kotton

   Article

   McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

   Insert

   sgRNA targeting human SCGB3A2 locus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-IRES-hrGFP-mWnt3a

   Plasmid

   #89771

   Purpose

   Expresses mouse Wnt3a in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Article

   Rodriguez et al J Neurosci. 2014 Jul 30;34(31):10285-97.

   Insert

   mouse Wnt3a (Wnt3a Mouse)
   

   Use

   AAV

   Tags

   None

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLQ7748 mU6: Spy sgTET || CMV: mCherry~P2A-DD-A4

   Plasmid

   #123657

   Purpose

   DD-AcrIIA4 fusion for Shield1-mediated AcrIIA4 control with sgRNA driving TRE3G activation.

   Depositor

   Stanley Qi

   Article

   Nakamura et al Nat Commun. 2019 Jan 14;10(1):194. doi:

   Insert

   mCherry-P2A-DD-AcrIIA4
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   mCherry-P2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and mU6

   Available since

   April 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. SOX17-NLS-tdTomato-EPG

   Plasmid

   #210466

   Purpose

   Donor plasmid for knock-in NLS-tdTomato into the human SOX17 locus

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Insert

   SOX17 homologous recombination arms with a NLS-tdTomato and EF1alpha-drived puromycin-EGFP selection cassette (SOX17 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. SNAP-ACE2

   Plasmid

   #178592

   Purpose

   Expresses SNAP-fused ACE2 protein that can be fluorescently labelled using SNAP substrates.

   Depositor

   Ralf Jockers

   Article

   Cecon et al Cell Chem Biol. 2021 Jul 2. pii: S2451-9

   Insert

   angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Flag and SNAP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3 2 kb prom. and exon 1 fragment CD274

   Plasmid

   #107007

   Purpose

   Modified Luciferase expression vector

   Depositor

   Julian Downward

   Article

   Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

   Insert

   CD274 (CD274 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEMS1991

    Plasmid

    #49122

    Purpose

    AAV plasmid with FEV (Ple67) promoter driving expression of iCre. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

    Insert

    ssAAV-Ple67-iCre-WPRE
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    FEV

    Available since

    May 6, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pCAG-FLEX-rc[Chronos-GFP]

    Plasmid

    #59056

    Purpose

    Expression vector for Chronos-GFP in a floxed/reversed, Cre-dependent manner (Cre turns gene on)

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDGB3 alpha2 P35S:MS2:VPR:Tnos (GB1830)

    Plasmid

    #160624

    Purpose

    TU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain VPR (VP64+p65+Rta)

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    P35S:MS2:VPR:Tnos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDGB3 alpha2 P35S:MS2:TV:Tnos (GB2048)

    Plasmid

    #160627

    Purpose

    TU for the constitutive expression of Ms2 fused to TV (TALx6 - VP128) activation domains

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    P35s-MS2:TV-Tnos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL4.23 WT 2014 EBOV Delta-Mucin-Like-Domain

    Plasmid

    #86021

    Purpose

    Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

    Depositor

    Pardis Sabeti, Jeremy Luban

    Article

    Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

    Insert

    Makona Ebola Virus Glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Lacks mucin-like domain

    Promoter

    CMV IE

    Available since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-Spy-CD86

    Plasmid

    #223598

    Purpose

    Lentiviral expression of human CD86 with SpyTag003 in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    CD86 (CD86 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    Spytag003

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMXs-WT-ATPIF1

    Plasmid

    #85403

    Purpose

    Retroviral vector expressing wild type human ATPIF1

    Depositor

    David Sabatini

    Article

    Chen et al Cell Rep. 2014 Apr 10;7(1):27-34. doi: 1

    Insert

    ATPIF1 (ATP5IF1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Wildtype

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

17. Flag Leucyl tRNA synthetase pRK5

    Plasmid

    #46341

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    leucyl tRNA synthetase (LARS1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

18. HR-TERT-SV40-GFP

    Plasmid

    #71397

    Purpose

    Homologues recombination donor plasmid fo the TERT promotor region introducing a SV-40 driven GFP marker in the TERT promotor. Includes 1kb of TERT coding region in right homologues arm.

    Depositor

    Thomas Cech

    Article

    Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

    Insert

    TERT (TERT Human)
    

    Use

    Tags

    SV-40 GFP insertion in TERT promotor

    Expression

    Mutation

    Promoter

    Endogenous TERT promotor

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGBT9-Hook3

    Plasmid

    #198525

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-Hook3 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

    Insert

    Hook3 (HOOK3 Human)
    

    Use

    Tags

    GAL4-DNA binding domain (BD) fragment

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR1A-TAZ S89A

    Plasmid

    #179911

    Purpose

    Entry vector of TAZ S89A for gateway coloning.

    Depositor

    Xin Chen

    Article

    Wang et al J Hepatol. 2021 Aug 28. pii: S0168-8278(

    Insert

    WW Domain Containing Transcription Regulator 1 (WWTR1 Human)
    

    Use

    Entry vector

    Tags

    3X Flag-tag

    Expression

    Mutation

    Serine 89 mutated to Alanine

    Promoter

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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