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pLN380 (FuGW-S(CEBPB)p-mKate2)

Plasmid

#105178

Purpose

synthetic promoter CEBPB drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(CEBPB)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pK3F

Plasmid

#85770

Purpose

N-ICE plasmid pK3F

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pMAL669

Plasmid

#169148

Purpose

Encodes OptoQ-AMP2

Depositor

Jose Avalos

Article

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL722

Plasmid

#169149

Purpose

Encodes OptoQ-AMP3

Depositor

Jose Avalos

Article

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Mutation

A79Q

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL716

Plasmid

#169150

Purpose

Encodes OptoQ-AMP4

Depositor

Jose Avalos

Article

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 C450A

Plasmid

#81031

Purpose

Mammalian expression of the dark state of LOV2

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV2

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

C450A

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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LZF80: Cre-on CAG-somCheRiff-CFP (DIO)

Plasmid

#104115

Purpose

synOptopatch construct

Depositor

Adam Cohen

Article

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

somCheRiff-CFP

Use

Lentiviral

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only
pMOTag-M9-4H

Plasmid

#106379

Purpose

Can be used as a PCR template to amplify the M9-HA point mutant of glmS fragment for gene specific tagging that only allows tagging but not regulation. Acts as a control for glmS based gene regulation

Depositor

Roberto Docampo

Article

Cruz-Bustos et al J Eukaryot Microbiol. 2017 Dec 19. doi:

Insert

GlmS with M9 mutation

Available Since

March 2, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pH3FS-GAL1

Plasmid

#85782

Purpose

N-ICE plasmid pH3FS-GAL1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-GAL1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs-Ecat1

Plasmid

#13351

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

ES cell associated transcript 1 (2410004A20Rik Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
3XFlag-pLPC

Plasmid

#73560

Purpose

vector encoding 3 Flag tags

Depositor

Gerardo Ferbeyre

Article

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

3X-Flag

Use

Retroviral

Tags

3X-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only
pILGFP4M

Plasmid

#104508

Purpose

Used to evaluate the expression output of Saccharomyces eubayanus GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SeGAL2 promoter-yEGFP

Expression

Yeast

Available Since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pJB028

Plasmid

#65469

Purpose

pTrc plasmid expressing p14*-TEV

Depositor

Keith Tyo

Article

Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

Insert

p14*-TEV (TEVgp1 )

Use

Synthetic Biology

Expression

Bacterial

Promoter

trc

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pGL2B -158/+64

Plasmid

#24946

Depositor

Stephen Smale

Article

Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

Insert

IL-10 promoter (Il10 Mouse)

Use

Luciferase

Tags

Luciferase

Available Since

June 4, 2010

Availability

Academic Institutions and Nonprofits only
pNUT3

Plasmid

#175744

Purpose

Single-component photo-inducible (CRY2-based) nucleolus-targeting tool 3

Depositor

Yubin Zhou

Article

Tan et al Nucleic Acids Res. 2022 Mar 23. pii: 655

Insert

mCherry-CRY2PHR-NoLS (human nuclear factor-κB inducing kinase)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pACYC_HA-ZmSAE1_ZmSAE2-FLAG

Plasmid

#91944

Purpose

Bacterial expression of maize SAE1 and SAE2

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

HA-ZmSAE1
ZmSAE2-FLAG

Tags

FLAG and HA

Expression

Bacterial

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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Vh-HA-2xFRB

Plasmid

#20224

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

2xFRB (MTOR Human)

Tags

HA and Vh

Expression

Mammalian

Mutation

One copy of FRB has a T2098L mutation

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
p3E-FKBP-HA no-pA

Plasmid

#82598

Purpose

HA epitope tag fused to C-terminus of human FK506 binding protein 12 (FKBP12) without pA for rapamycin/rapalog induced dimerization

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

FKBP-HA (no pA)

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pLN352 (FuGW-S(Elk1)p-mKate2)

Plasmid

#105175

Purpose

synthetic promoter Elk1 drives mKate2 experssion

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(Elk1)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCB150-2_GFP-EB1

Plasmid

#46364

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

EB1 (MAPRE1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

pBABE

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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