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pES007 (pTet-qacR 5/pQacA-deGFP)

Plasmid

#69036

Purpose

reporter with qacR 5 on single plasmid

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Inserts

qacR (qacR Synthetic)
deGFP

Tags

His6 tag

Expression

Bacterial

Mutation

W61Y, E90Q, F102S, M116Q, Y119L, E120Q

Promoter

pQacA and pTet

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pES003 (pTet-qacR 2)

Plasmid

#69032

Purpose

qacR 2 downstream of a Tet promoter

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Insert

qacR (qacR Synthetic)

Tags

His6 tag

Expression

Bacterial

Mutation

E57Q, E58L, W61Y, E90Q, I99Q, M116Q, L119Y, E120Q…

Promoter

pTet

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pET30-SBL1(160S,HisTagDel)

Plasmid

#66880

Purpose

Expresses a version of pET30Xa-SBL1(160S) with 41 bp Histag removed

Depositor

Betty Gaffney

Article

Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

Insert

soybean lipoxygenase-1 (S160S)

Tags

none

Expression

Bacterial

Mutation

none

Promoter

T7lac

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pET30-SBL1(160E,HisTagDel)

Plasmid

#66879

Purpose

Expresses a version of pET30Xa-SBL1(160E) with 41 bp Histag removed

Depositor

Betty Gaffney

Article

Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

Insert

soybean lipoxygenase-1 (S160E)

Expression

Bacterial

Mutation

S160E

Promoter

T7lac

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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mTFP1-SiT-N-15

Plasmid

#55508

Purpose

Localization: Golgi TGN, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

SiT (ST6GAL1 Human)

Tags

mTFP1

Expression

Mammalian

Mutation

aa 1-45 of SiT

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
mTFP1-Talin-ABM-10

Plasmid

#55510

Purpose

Localization: Talin Actin Binding Domain, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Talin ABM

Tags

mTFP1

Expression

Mammalian

Mutation

Actin Binding Module; I/LWEQ = 197 amino acids fr…

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pVRc01_3473

Plasmid

#49728

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

AS01_3473

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pLux

Available Since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pDD2013

Plasmid

#101364

Purpose

Name: pCBG68-K14ΔTATA. K14 TATA box mutation in pCBG68-LANApi (pDD2002).

Depositor

Dirk Dittmer

Article

Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

Insert

K14 TATA box mutation in pCBG68-LANApi (pDD2002).

Use

Unspecified

Mutation

K14 TATA box mutation in pCBG68-LANApi (pDD2002).

Availability

Academic Institutions and Nonprofits only

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pDD2022

Plasmid

#101366

Purpose

Name: pCBG68-RBPjkΔB. RBPjk binding site B mutation in pCBG68-LANApi (pDD2002).

Depositor

Dirk Dittmer

Article

Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

Insert

RBPjk binding site B mutation in pCBG68-LANApi (pDD2002).

Use

Luciferase

Mutation

RBPjk binding site B mutation in pCBG68-LANApi (p…

Availability

Academic Institutions and Nonprofits only

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pDD2026

Plasmid

#101369

Purpose

Name: pCBG68-LANApiΔTATA. LANApi TATA box mutation in pCBG68-LANApi (pDD2002).

Depositor

Dirk Dittmer

Article

Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

Insert

LANApi TATA box mutation in pCBG68-LANApi (pDD2002).

Use

Luciferase

Mutation

LANApi TATA box mutation in pCBG68-LANApi (pDD200…

Availability

Academic Institutions and Nonprofits only

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pDD2027

Plasmid

#101370

Purpose

Name: pCBR-LANApiΔTATA. LANApi TATA box mutation in pCBR-K14 (pDD2005).

Depositor

Dirk Dittmer

Article

Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

Insert

LANApi TATA box mutation in pCBR-K14 (pDD2005).

Use

Luciferase

Mutation

LANApi TATA box mutation in pCBR-K14 (pDD2005).

Availability

Academic Institutions and Nonprofits only

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EGFR WT

Plasmid

#11011

Purpose

Retroviral construct for expressing human EGFR in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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B1-INTEGRIN VN

Plasmid

#195216

Purpose

Mammalian expression of mCherry tagged B1 Integrin. Autoclustering mutant.

Depositor

David Bryant, Valerie Weaver

Article

Paszek et al Cancer Cell. 2005 Sep;8(3):241-54. doi:

Insert

ITGB1 (ITGB1 Human)

Expression

Mammalian

Mutation

V737N

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ROR2-V5/HIS

Plasmid

#205608

Purpose

Expression of human ROR2 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

ROR2 receptor tyrosine kinase (ROR2 Human)

Tags

V5-His

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pmirGLO:Δluc::gfp ΔAmpR::KanR

Plasmid

#176640

Purpose

MPRAu backbone vector

Depositor

Pardis Sabeti

Article

Griesemer et al Cell. 2021 Sep 30;184(20):5247-5260.e19.

Insert

emGFP

Expression

Mammalian

Promoter

Human PGK-1

Available Since

Feb. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-15F11-HA-mCh

Plasmid

#129591

Purpose

The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.

Depositor

Tim Stasevich

Article

Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

Insert

Anti-HA frankenbody-mCherry (15F11-HA scFv-mCh)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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JD633

Plasmid

#160393

Purpose

CRISPR-Cas9 vector including GRF4-GIF1. For wheat and other monocots CRISPR experiments. Includes two AarI sites for gRNA cloning

Depositor

Jorge Dubcovsky

Article

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Inserts

ZmUbi::wheat GRF4-GIF1 chimera
ZmUbi:TaCas9 + TaU6:gRNA

Use

Binary vector

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pET28 His6-mEGFP-D4H

Plasmid

#226373

Purpose

Production of recombinant mEGFP-D4H, a fluorescent probe binding cholesterol

Depositor

Catherine Tomasetto

Article

Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

Insert

D4H (pfoA Clostridium perfringens)

Tags

His6 and monomeric EGFP

Expression

Bacterial

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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NPY1R-DuET

Plasmid

#213354

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

NPY1R (NPY1R Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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OPRD1-DuET

Plasmid

#213358

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

OPRD1 (OPRD1 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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