4,272 results

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pLX313-Firefly luciferase

Plasmid

#118017

Purpose

Constitutive expression of Firefly luciferase

Depositor

William Hahn, David Root

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

Firefly luciferase

Use

Lentiviral

Expression

Mammalian

Promoter

EF1alpha

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pLV GG hUbC-dsRED

Plasmid

#84034

Purpose

Contains Golden Gate cassette to express up to four gRNAs and dsRED

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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JD633

Plasmid

#160393

Purpose

CRISPR-Cas9 vector including GRF4-GIF1. For wheat and other monocots CRISPR experiments. Includes two AarI sites for gRNA cloning

Depositor

Jorge Dubcovsky

Article

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Inserts

ZmUbi::wheat GRF4-GIF1 chimera
ZmUbi:TaCas9 + TaU6:gRNA

Use

Binary vector

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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tetO.Nfib.Hygro

Plasmid

#117271

Purpose

Expresses Nfib and hygromycin resistance gene under control of tetO sequence.

Depositor

Henrik Ahlenius

Article

Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

Insert

Nfib (Nfib Mouse)

Use

Lentiviral

Tags

IRES and hygromycin resistant cassette (C termina…

Expression

Mammalian

Promoter

tet-on

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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SepRS(2)/pSertRNA(B4)/EF-Sep

Plasmid

#173897

Purpose

The plasmid contains an orthogonal aminoacyl-tRNA synthetase/tRNACUA pair, which directs the efficient incorporation of phosphoserine (pSer) into recombinant proteins in Escherichia coli.

Depositor

Jason W Chin

Article

Rogerson et al Nat Chem Biol. 2015 Jul;11(7):496-503. d

Inserts

SepRS(2)
pSertRNA
EF-Sep

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2 neo

Plasmid

#98292

Purpose

Variant of lentiCRISPRv2 that confers G418 resistance

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

EF-1a

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCDH-hMALAT1

Plasmid

#118580

Purpose

to overexpress human MALAT1 in mammalian cells

Depositor

Jianjun Zhao

Article

Hu et al Leukemia. 2018 Mar 22. pii: 10.1038/s413

Insert

MALAT1 (MALAT1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

MSCV

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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ER-mNeonGreen

Plasmid

#137804

Purpose

Visualization of the Endoplasmic Reticulum

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

KDEL

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-EGFP-CNA35

Plasmid

#61603

Purpose

bacterial expression of N-terminal fusion protein of CNA35 with EGFP

Depositor

Maarten Merkx

Article

Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

Insert

EGFP and CNA35

Tags

His tags

Expression

Bacterial

Promoter

T7

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_AGIA-AirID

Plasmid

#182210

Purpose

Express AirID in mammalian cells.

Depositor

Sohei Ito, Shogo Nakano, Akira Nozawa, Tatsuya Sawasaki

Article

Kido et al Elife. 2020 May 11;9. pii: 54983. doi: 1

Insert

AGIA-AirID

Tags

AGIA-tag

Expression

Mammalian

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCasPA

Plasmid

#113347

Purpose

Bacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosa

Depositor

Quanjiang Ji

Article

Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pGRB

Plasmid

#71539

Purpose

for expression of gRNA in E. coli

Depositor

Tao Chen

Article

Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

Type

Empty backbone

Expression

Bacterial

Promoter

J231196

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-PEmax-P2A-hMLH1dn

Plasmid

#174828

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PEmax-P2A-hMLH1dn

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Detailed in manuscript; deletion of residues 754-…

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pYSEAP

Plasmid

#37326

Depositor

John Schiller

Article

Pastrana et al Virology. 2004 Apr 10;321(2):205-16.

Insert

SEAP

Expression

Mammalian

Promoter

EF1a

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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pdCas9-bacteria

Plasmid

#44249

Purpose

aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown

Depositor

Stanley Qi

Article

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

dCas9 (bacteria)

Use

CRISPR

Expression

Bacterial

Mutation

D10A H840A (catalytically inactive)

Promoter

pLtetO-1

Available Since

April 11, 2013

Availability

Academic Institutions and Nonprofits only

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HA GSK3 beta K85A pcDNA3

Plasmid

#14755

Depositor

Jim Woodgett

Article

Hughes et al EMBO J. 1993 Feb . 12(2):803-8.

Insert

GSK3 beta K85A (GSK3B Human)

Tags

HA

Expression

Mammalian

Mutation

K85A. Kinase dead mutant.

Available Since

April 12, 2007

Availability

Industry, Academic Institutions, and Nonprofits

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pRDA_174

Plasmid

#136476

Purpose

Lentiviral expression of EnAsCas12a

Depositor

John Doench

Article

DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

Insert

EnAsCas12a

Use

CRISPR and Lentiviral

Promoter

EF1a

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GenEPi

Plasmid

#140236

Purpose

Mammalian expression of Piezo1-based fluorescent force sensor GenEPi

Depositor

Periklis Pantazis

Article

Yaganoglu et al Nat Commun. 2023 Jul 19;14(1):4352. doi:

Insert

GenEPi (Piezo1-based fluorescent force sensor) (PIEZO1 Human)

Tags

GCaMP 6s RS1 EF4

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCW57.1-MAT2A

Plasmid

#100521

Purpose

Dox-repressible cDNA expression of MAT2A (dox-off/ tet-off all-in-one vector)

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

MAT2A (MAT2A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

pTight TRE

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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3xHA-miniTurbo-NLS_pCDNA3

Plasmid

#107172

Purpose

expresses 3xHA-tagged miniTurbo in the mammalian nucleus

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurbo (BirA mutant)

Expression

Mammalian

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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