19,755 results

1. pAAV-hSyn-GRAB-rDA3m

   Plasmid

   #208702

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m in neurons

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GPCR activation based dopamine (DA) sensor GRAB_rDA3m
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti.PGK.blast-Renilla_Luciferase

   Plasmid

   #74444

   Purpose

   Control lentiviral vector expressing Renilla luciferase

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   Renilla Luciferase
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. Apple-53BP1trunc

   Plasmid

   #69531

   Purpose

   Fluorescent reporter for DNA damage

   Depositor

   Ralph Weissleder

   Article

   Yang et al Sci Rep. 2015 May 18;5:10129. doi: 10.10

   Insert

   53BP1 (TP53BP1 Human)
   

   Use

   Lentiviral

   Tags

   Apple fluorescent protein

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-FLEx-ER-TurboID

   Plasmid

   #160857

   Purpose

   Cre recombinase-dependent expression of ER TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

   Depositor

   Jonathan Long

   Article

   Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

   Has Service

   AAV8

   Insert

   ER TurboID
   

   Use

   AAV

   Tags

   HA and V5

   Promoter

   CAG

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSP1E3D1

   Plasmid

   #20066

   Purpose

   Bacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   Denisov et al J Biol Chem. 2007 Mar 9. 282(10):7066-76

   Insert

   MSP1E3D1
   

   Tags

   7-His

   Expression

   Bacterial

   Mutation

   "extended" MSP1D1; contains repeats of …

   Available Since

   Jan. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV G-CEPIA1er

   Plasmid

   #58215

   Purpose

   Green fluorescent indicator for calcium imaging in the endoplasmic reticulum

   Depositor

   Masamitsu Iino

   Article

   Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

   Insert

   G-CEPIA1er
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   G-GECO1.1 E299D M304L D328N N345K F360W G369S E37…

   Promoter

   CMV

   Available Since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. M51 Super 8x FOPFlash (TOPFlash mutant)

   Plasmid

   #12457

   Purpose

   Control for beta-catenin reporter. Contains mutated TCF/LEF binding sites upstream of a luciferase reporter.

   Depositor

   Randall Moon

   Article

   Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

   Insert

   mutant TCF/LEF binding sites (Ctnnb1 )
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   mutant TCF/LEF binding sites

   Available Since

   March 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpyCatcher003-sfGFP

   Plasmid

   #133449

   Purpose

   Expresses SpyCatcher003-superfolder GFP in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyCatcher003-sfGFP
   

   Tags

   His6 and TEV site

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLJM1-FIRE-pHLy

   Plasmid

   #170775

   Purpose

   Ratiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cells

   Depositor

   Aimee Kao

   Article

   Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse

   Insert

   LAMP1 (LAMP1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry and mTFP1

   Expression

   Mammalian

   Mutation

   84 nucleotide Signal Sequence switched to the beg…

   Promoter

   CMV

   Available Since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-F-RAM-d2tTA-TRE-mKate2

    Plasmid

    #140274

    Purpose

    F-RAM, the Fos-dependent reporter

    Depositor

    Yingxi Lin

    Article

    Sun et al Cell. 2020 Apr 16;181(2):410-423.e17. do

    Insert

    mKate2
    

    Use

    AAV

    Promoter

    TRE

    Available Since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. CD44S pBabe-puro

    Plasmid

    #19127

    Depositor

    Bob Weinberg

    Article

    Godar et al Cell. 2008 Jul 11. 134(1):62-73.

    Insert

    CD44 standard (CD44 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9_T2A_mCherry sg1-sg2

    Plasmid

    #192479

    Purpose

    All-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Tags

    T2A mCherry

    Expression

    Mammalian

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pML107

    Plasmid

    #67639

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation

    Depositor

    John Wyrick

    Article

    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

    Inserts

    Cas9
    single guide RNA expression cassette
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    pSNR52 and pTDH3 (aka GAP promoter)

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSCV IRES Luciferase

    Plasmid

    #18760

    Depositor

    Scott Lowe

    Type

    Empty backbone

    Use

    Luciferase and Retroviral

    Expression

    Mammalian

    Available Since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)

    Plasmid

    #139998

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

    Promoter

    CAG

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP

    Plasmid

    #50946

    Purpose

    An empty gRNA expression vector

    Depositor

    Kosuke Yusa

    Article

    Koike-Yusa et al Nat Biotechnol. 2014 Mar;32(3):267-73. d

    Insert

    None
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS2+HyPer7

    Plasmid

    #136466

    Purpose

    Mammalian expression of non-targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Expression

    Mammalian

    Promoter

    CMV, SP6

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221_SLC7A11

    Plasmid

    #132244

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC7A11 (SLC7A11 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 21, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. GFP-MAPPER

    Plasmid

    #117721

    Purpose

    MAPPER Selectively Labels ER-PM Junctions

    Depositor

    Jen Liou

    Article

    Chang et al Cell Rep. 2013 Nov 14;5(3):813-25. doi:

    Insert

    MAPPER
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLentiCRISPRv2-STING_gRNA3

    Plasmid

    #127640

    Purpose

    Knock-out of human STING

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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