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pENTR2b-EGFP

Plasmid

#215537

Purpose

Gateway entry vector with the EGFP fluorescent protein

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

EGFP

Use

Gateway entry vector

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pEN_TmiRc3

Plasmid

#25748

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE promoter.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pMF1707

Plasmid

#125584

Purpose

Expresses cytosolic roGFP2 (c-roGFP2) in mammalian cells

Depositor

Marc Fransen

Article

Ivashchenko et al Mol Biol Cell. 2011 May;22(9):1440-51. d

Insert

c-roGFP2

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-F0ATPMLS-mCherry-GFP1-10

Plasmid

#211466

Purpose

Lentivirus backbone expressing mCherry and GFP1-10 fusion protein with a mitochondrial localization signal

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

mito-mCherry-GFP1-10

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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bRA89

Plasmid

#100950

Purpose

Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HPH marker

Depositor

James Haber

Article

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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PRExpress-Bxb1-hsp70pA

Plasmid

#158391

Purpose

heat shock inducible Bxb1 integrase for drosophila melanogaster

Depositor

Michael Elowitz

Article

Chow et al Science. 2021 Apr 9;372(6538). pii: 372/

Insert

Bxb1

Expression

Insect

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEN_TTmcs

Plasmid

#25755

Purpose

Entry vector with TRE-tight promoter followed by multiple cloning site.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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AviTag-SpyCatcher

Plasmid

#72326

Purpose

Bacterial expression of SpyCatcher with a peptide tag enabling site-specific biotinylation.

Depositor

Mark Howarth

Article

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

AviTag-SpyCatcher

Tags

N-term His6 tag and TEV cleavage site

Expression

Bacterial

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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UAS-Ceru-10unit

Plasmid

#158389

Purpose

10-unit intMEMOIR recording array for Drosophila melanogaster

Depositor

Michael Elowitz

Article

Chow et al Science. 2021 Apr 9;372(6538). pii: 372/

Insert

10unit recording array

Expression

Insect

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pX330_DDX4-cterm

Plasmid

#222520

Purpose

Cas9/sgRNA plasmid for targeting DDX4

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

Cas9, DDX4 sgRNA (DDX4 Synthetic, Human)

Use

CRISPR

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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dCas9-AsLOV2x3-NLSx3

Plasmid

#231566

Purpose

Expresses components of the LOOMINA optogenetic transcriptional control system.

Depositor

Dominik Niopek

Article

Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

Insert

dCas9-AsLOV2x3

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pBabe NEO mRFP1

Plasmid

#98397

Purpose

Retroviral vector for constitutive mRFP1 expression

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

mRFP1

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Oct4

Plasmid

#19766

Depositor

Konrad Hochedlinger

Article

Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.

Insert

Oct4 (Pou5f1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pUAS-syb:spGFP1–10

Plasmid

#73698

Purpose

Neuronal-synaptobrevin (syb):spGFP1–10 chimera for synaptic GRASP

Depositor

Marco Gallio

Article

Macpherson et al Nat Commun. 2015 Dec 4;6:10024. doi: 10.

Insert

Neuronal-synaptobrevin:spGFP1–10 chimera

Use

Drosophila

Expression

Insect

Available Since

March 22, 2016

Availability

Academic Institutions and Nonprofits only

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GpACR1-pcDNA3.1-mScarlet

Plasmid

#204964

Purpose

Expression of GpACR1 in fusion with mScarlet in mammalian cells

Depositor

Peter Hegemann

Article

Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

Insert

GpACR1

Tags

mScarlet

Expression

Mammalian

Promoter

CMV (+ enhancer)

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD/His-miRFP703

Plasmid

#79985

Purpose

Monomeric near-infrared fluorescent protein miRFP703 in bacterial plasmid

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

miRFP703

Expression

Bacterial

Promoter

araB

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pEX.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP

Plasmid

#45565

Purpose

Expresses I-Sce I nuclease in mammalian cells and BFP as a tracker

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Inserts

I-Sce I Nuclease
BFP

Tags

HA

Expression

Mammalian

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pEN_Tmcs

Plasmid

#25751

Purpose

Entry vector with TRE promoter followed by multiple cloning site.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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BlatCas9

Plasmid

#163792

Purpose

The plasmid expresses human codon-optimized BlatCas9 and gRNA expression elements.

Depositor

Yongming Wang

Article

Gao et al Front Cell Dev Biol. 2020 Oct 19;8:58316

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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bRA90

Plasmid

#100951

Purpose

Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. LEU2 marker

Depositor

James Haber

Article

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pMF1762

Plasmid

#125583

Purpose

Expresses mitochondrial roGFP2 (mt-roGFP2) in mammalian cells

Depositor

Marc Fransen

Article

Ivashchenko et al Mol Biol Cell. 2011 May;22(9):1440-51. d

Insert

mt-roGFP2

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMM117

Plasmid

#127212

Purpose

BeYDV replicon with constitutive Luc expression, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pBbS9a-RFP

Plasmid

#126172

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23113

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pEN_TRE_GFP

Plasmid

#25767

Purpose

Entry vector with TRE promoter driving GFP expression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Tags

GFP

Available Since

July 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti-PRSX8 eArchT3.0-EYFP

Plasmid

#89538

Purpose

Expresses eArchT 3.0-eYFP in Phox2 (including catecholamine) neurons

Depositor

Ruth Stornetta

Article

Burke et al J Physiol. 2015 Jul 1;593(13):2909-26. d

Insert

eArchT 3.0

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Promoter

PRSx8

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pEN_TTmiRc2

Plasmid

#25752

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE-tight promoter.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pLX302 LacZ-V5 puro

Plasmid

#98579

Purpose

Lentiviral vector for constitutive expression of human LacZ with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

LacZ

Use

Lentiviral

Tags

2x V5 tag

Expression

Mammalian

Available Since

Aug. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pBbB19c-GFP

Plasmid

#126139

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23105

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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34a_PU1 in pmiRGlo

Plasmid

#78131

Purpose

luciferase reporter for miRNA activity

Depositor

Heidi Schwarzenbach

Article

Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

Insert

PU1

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

3'UTR containing miRNA binding sites

Promoter

PGK

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pSW002-Pc

Plasmid

#108234

Purpose

Broad host bacterial expression vector with constitutive Pc promoter.

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

Pc promoter

Expression

Bacterial

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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