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Plasmid#182852PurposeHetero-dimer expression vector with plasma membrane localization signalDepositorInsertmyr-palm-mEYFP-mEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
HEK3_pegF_18bp
Plasmid#174852PurposeExpress forward pegRNA mediating 991-bp deletion and 18-bp insertionDepositorInsertHEK3_pegF_18bp insertion
UseTagsExpressionMammalianMutationPromoterU6Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
HEK3_pegR_18bp
Plasmid#174853PurposeExpress reverse pegRNA mediating 991-bp deletion and 18-bp insertionDepositorInsertHEK3_pegR_18bp
UseTagsExpressionMammalianMutationPromoterU6Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
HEK3_pegF_44bp
Plasmid#174854PurposeExpress forward pegRNA mediating 991-bp deletion and 44-bp insertionDepositorInsertHEK3_pegF_44bp insertion
UseTagsExpressionMammalianMutationPromoterU6Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
HEK3_pegR_44bp
Plasmid#174855PurposeExpress forward pegRNA mediating 991-bp deletion and 44-bp insertionDepositorInsertHEK3_pegR_44bp insertion
UseTagsExpressionMammalianMutationPromoterU6Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDYU1H-TED
Plasmid#154300PurposeA expression vector in Dictyostelium discoideum containing the Hygromycin B resistant gene and the TED module for GFP-negative screeningDepositorTypeEmpty backboneUseDictyostelium expressionTagsExpressionMutationPromoteractin15Available sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDYU1B-TED
Plasmid#154299PurposeA expression vector in Dictyostelium discoideum containing the Blasticidin S resistant gene and the TED module for GFP-negative screeningDepositorTypeEmpty backboneUseDictyostelium expressionTagsExpressionMutationPromoteractin15Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJOE4847.2
Plasmid#135086Purposerhamnose-inducible expression vectorDepositorInsertMalE-6xHis-Ulp1
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
GGE286
Plasmid#120776PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 3 (T3_Tef)DepositorInsertT3_Tef
UseCloning/assembly plasmidTagsExpressionMutationPromoterAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
GGE256
Plasmid#120772PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 2 (T2_Tef)DepositorInsertT2_Tef
UseCloning/assembly plasmidTagsExpressionMutationPromoterAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
GGE082
Plasmid#120767PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 1 (T1_Tef)DepositorInsertT1_Tef
UseCloning/assembly plasmidTagsExpressionMutationPromoterAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
GGE007
Plasmid#120758PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position 3 (P3_PGM)DepositorInsertP3_PGM
UseCloning/assembly plasmidTagsExpressionMutationPromoterAvailable sinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
GGE008
Plasmid#120757PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position 3 (P3_GAPdh)DepositorInsertP3_GAPdh
UseCloning/assembly plasmidTagsExpressionMutationPromoterAvailable sinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0519
Plasmid#128982PurposePart plasmid for loxP-KIURA3-loxP, Type 6 (yeast marker) partDepositorInsertloxP-KIURA3-loxP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
GGE005
Plasmid#120748PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position 2 (P2_GAPdh)DepositorInsertP2_GAPdh
UseCloning/assembly plasmidTagsExpressionMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
GGE004
Plasmid#120749PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position 2 (P2_PGM)DepositorInsertP2_PGM
UseCloning/assembly plasmidTagsExpressionMutationPromoterAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-S. tuberosum trnV/3'rps12
Plasmid#123585PurposeMoChlo kitDepositorInsertS. tuberosum trnV/3'rps12 integration site
UseChloroplast transformation vectorTagsExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-S. tuberosum trnG/trnfM
Plasmid#123584PurposeMoChlo kitDepositorInsertS. tuberosum trnG/trnfM integration site
UseChloroplast transformation vectorTagsExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-N. tabacum trnV/3'rps12
Plasmid#123581PurposeMoChlo kitDepositorInsertN. tabacum trnV/3'rps12 integration site
UseChloroplast transformation vectorTagsExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-N. tabacum trnG/trnfM
Plasmid#123580PurposeMoChlo kitDepositorInsertN. tabacum trnG/trnfM integration site
UseChloroplast transformation vectorTagsExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-Lux-G codon opt. Nt chloroplasts
Plasmid#123568PurposeMoChlo kitDepositorInsertLux-G codon optimized Nt chloroplasts
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-Lux-E codon opt. Nt chloroplasts
Plasmid#123567PurposeMoChlo kitDepositorInsertLux-E codon optimized Nt chloroplasts
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-Lux-B codon opt. Nt chloroplasts
Plasmid#123566PurposeMoChlo kitDepositorInsertLux-B codon optimized Nt chloroplasts
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-Lux-A codon opt. Nt chloroplasts
Plasmid#123565PurposeMoChlo kitDepositorInsertLux-A codon optimized Nt chloroplasts
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-Lux-D codon opt. Nt chloroplasts
Plasmid#123564PurposeMoChlo kitDepositorInsertLux-D codon optimized Nt chloroplasts
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-Lux-C codon opt. Nt chloroplasts
Plasmid#123563PurposeMoChlo kitDepositorInsertLux-C codon optimized Nt chloroplasts
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-psbA-rbcL chimeric 5'UTR (R'SLWT)
Plasmid#123543PurposeMoChlo kitDepositorInsertpsbA-rbcL chimeric 5'UTR (R'SLWT)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-psbA-rbcL chimeric 5'UTR (R delta 5'SL)
Plasmid#123542PurposeMoChlo kitDepositorInsertpsbA-rbcL chimeric 5'UTR (R delta 5'SL)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-psbH 5'UTR + start codon + 1AA
Plasmid#123537PurposeMoChlo kitDepositorInsertpsbH 5'UTR + start codon + 1AA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-rps2 5'UTR mCCUC (-20 to -10 deletion)
Plasmid#123536PurposeMoChlo kitDepositorInsertrps2 5'UTR mCCUC (-20 to -10 deletion)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-rps2 5'UTR mAAGA (-20 to -10 deletion)
Plasmid#123535PurposeMoChlo kitDepositorInsertrps2 5'UTR mAAGA (-20 to -10 deletion)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-atpB 5'UTR TCR processed -90 (functional)
Plasmid#123523PurposeMoChlo kitDepositorInsertatpB 5'UTR TCR processed -90 (functional)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-atpB 5'UTR +10 processed -90 (functional)
Plasmid#123522PurposeMoChlo kitDepositorInsertatpB 5'UTR +10 processed -90 (functional)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-mPrrn (5-fold decrease transcription)
Plasmid#123478PurposeMoChlo kitDepositorInsertmPrrn (5-fold decrease transcription)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-PatpB (PEP functional NEP disabled)
Plasmid#123469PurposeMoChlo kitDepositorInsertPatpB (PEP functional NEP disabled)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-PatpB (PEP and NEP functional)
Plasmid#123468PurposeMoChlo kitDepositorInsertPatpB (PEP and NEP functional)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK-PatpB (NEP functional PEP disabled)
Plasmid#123466PurposeMoChlo kitDepositorInsertPatpB (NEP functional PEP disabled)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pS1GG
Plasmid#108197PurposeS. solfataricus PCNA1DepositorInsertS. solfataricus PCNA1
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pS3GG
Plasmid#108199PurposeS. solfataricus PCNA3DepositorInsertS. solfataricus PCNA3
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pS2GG
Plasmid#108198PurposeS. solfataricus PCNA2DepositorInsertS. solfataricus PCNA2
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pM2GG
Plasmid#108201PurposeM. sedula PCNA2DepositorInsertM. sedula PCNA2
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pM3GG
Plasmid#108202PurposeM. sedula PCNA3DepositorInsertM. sedula PCNA3
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBsaG4SPdX
Plasmid#108203PurposeG4S-PdXDepositorInsertPutidaredoxin
UseTagsExpressionBacterialMutationchanged Cysteine 73 to Serine and Cysteine 85 to …Promoterlac promoterAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pECT1B
Plasmid#75447PurposePlasmid that serves as the PCR template to create genomic pdt1B insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt1B
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pECT1D
Plasmid#75448PurposePlasmid that serves as the PCR template to create genomic pdt1D insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt1D
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pECT1E
Plasmid#75449PurposePlasmid that serves as the PCR template to create genomic pdt1E insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt1E
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIMP1-Pthl-AbrB3647
Plasmid#80981PurposeUsed for abrB3647(CAC3647) overexpression in Clostridium acetobutylicumDepositorInsertabrB3647(CAC3647)
UseTagsExpressionBacterialMutationPromoterthl (cac2873)Available sinceSept. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIMP1-Pthl-AbrB1941
Plasmid#80980PurposeUsed for abrB1941(CAC1941) overexpression in Clostridium acetobutylicumDepositorInsertabrB1941(CAC1941)
UseTagsExpressionBacterialMutationPromoterthl (cac2873)Available sinceSept. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIMP1-Pthl-AbrB0310
Plasmid#80979PurposeUsed for abrB0310(CAC0310) overexpression in Clostridium acetobutylicumDepositorInsertabrB0310(CAC0310)
UseTagsExpressionBacterialMutationPromoterthl (cac2873)Available sinceSept. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -