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We narrowed to 19,311 results for: Mut

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Showing: 8201 - 8220 of 19311 results

  6. pDEST-Myc-FBW7]K404R

    Plasmid
    #200715
    Purpose
    The plasmid expresses Myc-tagged FBW7 human isoform 1 with Lys404 to Arg mutation. Used for overexpression and detection by western blotting.
    Depositor
    Omar Khan
    Article
    Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:
    Insert
    FBW7 (FBXW7 Human)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    FBW7 K404 is mutated to Alanine.
    Promoter
    CMV
    Available Since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    200715-plasmid-map-sequence-id-394919

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  8. pBactin-Nedd1-mOrange2-F75A

    Plasmid
    #196862
    Purpose
    Expression of Need1-rCM1 harboring a (F75A) mutation in the rat (r) Centrosomin motif 1 (CM1) and fused to mOrange2. F75A mutatuon renders CM1 incapable of binding and activating gamma-TuRC
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    Nedd1-mOrange2-F75A (Nedd1 Rat)
    Expression
    Mammalian
    Mutation
    F75A in the CM1 domain
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available Since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196862-plasmid-map-sequence-id-391831

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  12. eGFP-proα2(I)-G610C

    Plasmid
    #119827
    Purpose
    Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and GFP between signal sequence and exon 6
    Depositor
    Sergey Leikin
    Article
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Insert
    Type I procollagen α2 chain (Col1a2 Mouse)
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Col1a2 exons 2-5 replaced by fluorescent tag, Gly…
    Promoter
    CMV
    Available Since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119827-plasmid-map-sequence-id-233848

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  14. A24M04 mIgM heavy chain-pFEEKW

    Plasmid
    #234809
    Purpose
    Express membrane form of IgM heavy chain containing the VH region of the monoclonal antibody, A24M04, specific for human papilloma virus16 L1
    Depositor
    Susan Pierce
    Article
    Torgbor et al Proc Natl Acad Sci U S A. 2025 Mar 11;12
    Insert
    A24M04 membrane IgM heavy chain (IGHM Human)
    Use
    Lentiviral
    Expression
    Bacterial and Mammalian
    Promoter
    EEK promoter
    Available Since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    234809-plasmid-map-sequence-id-466813

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  16. pACEBac1-PARP1-Flag-His-7xKQ

    Plasmid
    #111573
    Purpose
    Expresses full length PARP1 with K to Q mutations in automodification domain in Sf9 (insect) cells
    Depositor
    Thomas Muir
    Article
    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038
    Insert
    PARP1 (PARP1 Human)
    Tags
    C-terminal 6xHis and C-terminal Flag
    Expression
    Insect
    Mutation
    lysine to glutamine mutation at the following ami…
    Promoter
    polyhedrin promoter
    Available Since
    July 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    111573-plasmid-map-sequence-id-215588

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  17. pMini-CMV-NLS-R-IscB-VR4-NLS (D60A, H243E, H269N, R270Q)_T2A_mCherry_U6-ωRNA

    Plasmid
    #246431
    Purpose
    All-in-one plasmid. Expresses R-IscB in mammalian cells. ssRNase enhancing mutations (H243E, H269N, R270Q) included.
    Depositor
    Ailong Ke
    Article
    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.
    Inserts
    O.gue IscB-VR4 (H243E, H269N, R270Q) with RuvC dead mutations (D60A) and delta-TID
    mCherry
    ωRNA
    Tags
    SV40 NLS, Thosea asigna virus 2A peptide, and nuc…
    Expression
    Mammalian
    Mutation
    changed Aspartic Acid 60 to Alanine, changed Hist…
    Promoter
    CMV and U6
    Available Since
    Jan. 6, 2026
    Availability
    Academic Institutions and Nonprofits only
    246431-plasmid-map-sequence-id-496263

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  18. pBFC1171

    Plasmid
    #231994
    Purpose
    CRISPRi-ART crRNA only plasmid encoding crystal violet-inducible crRNA (for dRfxCas13d) with 2xBsaI spacer cloning site
    Depositor
    Brady Cress
    Article
    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d
    Insert
    crRNA of dRfxCas13d
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    pJEx (Jungle Express)
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    231994-plasmid-map-sequence-id-468506

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  19. PB-NES-ZapCV2

    Plasmid
    #222950
    Purpose
    Genetically-encoded Cytosolic Cyan-Yellow Zinc FRET sensor. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)
    Depositor
    Amy Palmer
    Article
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Insert
    NES-ZapCV2
    Tags
    ECFP (Enhanced Cyan Fluorescent Protein), Nuclear…
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222950-plasmid-map-sequence-id-450583

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Showing: 8201 - 8220 of 19311 results