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Showing: 8261 - 8280 of 9023 results

  1. lentiGuide-Hygro-dTomato

    Plasmid
    #99376
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Kristen Brennand
    Article
    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6
    Inserts
    S. pyogenes sgRNA cassette
    Hygro-P2A-dTomato
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a and hU6
    Available Since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    99376-plasmid-map-sequence-id-191027

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  3. pLSB-KAN

    Plasmid
    #166700
    Purpose
    Combined sgRNA/Cas9 pLSB vector with kanMX6 (G418) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Article
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Expression
    Yeast
    Promoter
    adh15 promoter and tRNA Ser promoter
    Available Since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166700-plasmid-map-sequence-id-329224

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  4. pROF148

    Plasmid
    #155330
    Purpose
    Binary vector for expression of the PULSE system under the control of AtUbi10 promoter. It contains a plant selection cassette. 
    Depositor
    Matias Zurbriggen
    Article
    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi
    Insert
    LB_Tnos-nptII-Pnos_PAtUbi10-SRDX-NLS-EL222-Tnos_PAtUbi10-E-PIF6-NLS-Tnos_PAtUbi10-PhyB-VP16-NLS-Tnos_RB
    Use
    Synthetic Biology
    Expression
    Plant
    Available Since
    Oct. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    155330-plasmid-map-sequence-id-311083

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  6. pLSB-HYG

    Plasmid
    #166699
    Purpose
    Combined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Article
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Expression
    Yeast
    Promoter
    adh15 promoter and tRNA promoter
    Available Since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166699-plasmid-map-sequence-id-474294

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  8. lentiGuide-Crimson

    Plasmid
    #70683
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Crimson reporter from EF-1a promoter. Lentiviral backbone.
    Depositor
    Daniel Bauer
    Article
    Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature
    Inserts
    S. pyogenes sgRNA cassette
    Crimson Reporter
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    Ef1-a and hU6
    Available Since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    70683-plasmid-map-sequence-id-217910

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  9. iCas

    Plasmid
    #84232
    Purpose
    Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)
    Depositor
    Meng How Tan
    Article
    Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038
    Insert
    Cas9
    Tags
    2A-OFP co-expression and ERT2-ERT2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    84232-plasmid-map-sequence-id-323254

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  10. PC1575

    Plasmid
    #232088
    Purpose
    Mammalian expression plasmid for ubiquibody (uAb) cloning backbone. Includes an Esp3I restriction site directly upstream of GSGSG linker and CHIPΔTPR CDS and a C-terminal IRES-mCherry cassette.
    Depositor
    Pranam Chatterjee
    Article
    Bhat et al Sci Adv. 2025 Jan 24;11(4):eadr8638. doi
    Insert
    uAb with cloning Esp3I restriction site
    Tags
    IRES-mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    232088-plasmid-map-sequence-id-464073

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  11. pAS_4xU6-PylT M15 (UUA) FLAG-Mma PylRS

    Plasmid
    #154774
    Purpose
    plasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and Mma PylRS for ochre suppression in transient or stable, piggybac-mediated, integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi
    Insert
    Mma PylRS (pylS Methanosarcina mazei)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    in PylT: UUA anticodon and G10A, G14U, G16U, C20U…
    Promoter
    EF1
    Available Since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    154774-plasmid-map-sequence-id-310423

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  14. pHtrA-TEV-His12

    Plasmid
    #137036
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi HtrA with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66500.2 (BB_0104 Borrelia burgdorferi B31)
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available Since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137036-plasmid-map-sequence-id-275699

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  15. pLSB-NAT

    Plasmid
    #166698
    Purpose
    Combined sgRNA/Cas9 pLSB vector with natMX6 (cloNAT) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Article
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Expression
    Yeast
    Promoter
    adh15 promoter and tRNA promoter
    Available Since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166698-plasmid-map-sequence-id-329407

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  16. lentiGuide-Hygro-iRFP670

    Plasmid
    #99377
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A iRFP670 from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Kristen Brennand
    Article
    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6
    Inserts
    S. pyogenes sgRNA cassette
    Hygro-P2A-iRFP670
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a and hU6
    Available Since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    99377-plasmid-map-sequence-id-191028

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  19. pAS_4xEcoLeuT(CUA)_AnapRS

    Plasmid
    #140019
    Purpose
    Plasmid with 4xEcoLeuT(CUA) cassette and E. coli AnapRS for amber suppression and incorporation of the fluorescent ncAA Anap; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006
    Insert
    EcoLeuRS (AnapRS)
    Expression
    Mammalian
    Mutation
    L38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…
    Promoter
    EF1
    Available Since
    May 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140019-plasmid-map-sequence-id-297114

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  20. PB-CAG-dCasRx-RBM25-IRES-BleoR

    Plasmid
    #221002
    Purpose
    Stable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.
    Depositor
    Benjamin Blencowe, Mikko Taipale
    Article
    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e
    Insert
    dCasRx-RBM25-IRES-BleoR
    Use
    CRISPR
    Tags
    NLS and NLS-HA
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    221002-plasmid-map-sequence-id-436162

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Showing: 8261 - 8280 of 9023 results