We narrowed to 16,583 results for: EMB

1. mCherry-p62-Silent_Mutation_WT

   Plasmid

   #187986

   Purpose

   human p62 with silent mutation for sip62 #5 (Thermo Scientific). Internal reference: SMC516

   Depositor

   Sascha Martens

   Article

   Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

   Insert

   p62 (NUP62 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   silent mutation for resistance to sip62 #5

   Promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-mouse vinculin full length (889)

   Plasmid

   #67935

   Purpose

   Mammalian expression of mouse vinculin with GFP fusion

   Depositor

   Alpha Yap

   Article

   Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

   Insert

   Vinculin (Vcl Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   L159P in vinculin

   Promoter

   CMV

   Available since

   Nov. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-E2F4

   Plasmid

   #10914

   Depositor

   Bob Weinberg

   Article

   Sardet et al Proc Natl Acad Sci U S A. 1995 Mar 14. 9

   Insert

   E2F4 (E2F4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 11, 2005

   Availability

   Academic Institutions and Nonprofits only

4. pDULE-ABK

   Plasmid

   #49086

   Purpose

   A pyrrolysyl tRNA/tRNA sythetase pair evolved to efficiently incorporate an aliphatic diazirine amino acid into proteins in E. coli and mammalian cells for crosslinking experiments

   Depositor

   Peter Schultz

   Article

   Ai et al Chembiochem. 2011 Aug 16;12(12):1854-7.

   Inserts

   tRNA synthetase
   tRNA for a photocrosslinking amino acid
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   L274M, C313A, Y349F

   Promoter

   GlnRS and lpp

   Available since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pK.myc-MYPT1

   Plasmid

   #24101

   Depositor

   Masumi Eto

   Article

   Eto et al Cell Motil Cytoskeleton. 2005 Oct . 62(2

   Insert

   Myosin-targeting subunit-1 (PPP1R12A Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   S50G, P415Q

   Promoter

   Available since

   March 19, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMVHA E2F2

   Plasmid

   #24226

   Purpose

   Mammalian expression of human E2F2 with HA tag

   Depositor

   Kristian Helin

   Article

   Lukas et al Mol Cell Biol. 1996 Mar . 16(3):1047-57.

   Insert

   E2F2 (E2F2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOPTG-Tau-RING

   Plasmid

   #233691

   Purpose

   Expression of Tau-RING protein in bacteria

   Depositor

   Leo James

   Article

   Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

   Insert

   pOPTG
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBITE-EGFRvIII-F263-OKT3

   Plasmid

   #190680

   Purpose

   Encodes an EGFRvIII-CD3 BITE protein (F263 clone)

   Depositor

   Scott McComb

   Article

   Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

   Insert

   Anti-EGFRvIII-scFv-F263-modularized-OKT3-HisTag
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. p5E-mmCdh5pro (JDW 1239)

   Plasmid

   #224478

   Purpose

   Gateway compatible 5' entry clone containing the Murine Cdh5 promoter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   Cdh5 promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. BFP-Dcp1a

    Plasmid

    #153973

    Purpose

    Fluorescent protein fused to mRNA decapping enzyme 1a

    Depositor

    Gia Voeltz

    Article

    Lee et al Science. 2020 Jan 31;367(6477). pii: 367

    Insert

    mRNA decapping enzyme 1a (DCP1A Human)
    

    Use

    Tags

    mTagBFP-c1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. PDZD7-TwinStrepII

    Plasmid

    #83198

    Purpose

    Expression of tagged protein in mammalian cells

    Depositor

    Peter Barr-Gillespie

    Article

    Morgan et al Elife. 2016 Aug 15;5:e18312. doi: 10.755

    Insert

    PDZD7 (Pdzd7 Mouse)
    

    Use

    Tags

    Twin Strep Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCherry-FKBP-PI4KAC1001(D1957A)

    Plasmid

    #139312

    Purpose

    Recruitable PI4KA kinase-dead catalytic fragment

    Depositor

    Gerry Hammond

    Article

    Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    mCherry: FKBP1A(3-108): GGSA]4GG:PI4KA(1102-2103) (PI4KA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D1957A, G1986D

    Promoter

    Available since

    April 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-SARS-CoV-2-M

    Plasmid

    #215813

    Purpose

    Expresses SARS-CoV-2 Membrane protein in mammalian cells

    Depositor

    Thomas Gallagher

    Article

    Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

    Insert

    SARS-CoV-2 Membrane (M )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-EKAR4

    Plasmid

    #174437

    Purpose

    Improved cyan/yellow FRET-based ERK kinase activity reporter.

    Depositor

    Jin Zhang

    Article

    Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

    Insert

    EKAR4
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-ChroME-mRuby2-ST

    Plasmid

    #108902

    Purpose

    Cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic excitation

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    ChroME-mRuby2-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAGH42

    Plasmid

    #107249

    Purpose

    Vipp1-mGFPmut3 (or vipp1-gfp) construct with spectinomycin resistance cassette downstream used to replace the native copy of Vipp1 in Synechocystis (sll0617).

    Depositor

    Erin O'Shea

    Article

    Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111

    Insert

    vipp1-gfp
    

    Use

    Suicide vector for destined for double homologous…

    Tags

    mGFPmut3

    Expression

    Bacterial

    Mutation

    Promoter

    native promoter of sll0617

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Rtn4a-mNeonGreen

    Plasmid

    #169032

    Purpose

    Express human Rtn4a

    Depositor

    Gia Voeltz

    Article

    Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

    Insert

    Rtn4a (RTN4 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET15-His-∆NGlpG

    Plasmid

    #155131

    Purpose

    Expresses N-terminally His-tagged E. coli GlpG (residues 87-276) from pET15b

    Depositor

    Sinisa Urban

    Article

    Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

    Insert

    GlpG (glpG Escherichia coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    insert codes for GlpG residues 87-276

    Promoter

    T7

    Available since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE-3FLAG-RAB5A

    Plasmid

    #176488

    Purpose

    For lentiviral transduction of 3FLAG-RAB5A

    Depositor

    Wade Harper

    Article

    Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

    Insert

    RAB5A (RAB5A Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-Lyn-dInPAkt

    Plasmid

    #181834

    Purpose

    Genetically encoded single-color sensor for monitoring plasma membrane PI(3,4,5)P2 dynamics in living cells. Based on dimerization-dependent red fluorescent protein (ddRFP).

    Depositor

    Jin Zhang

    Article

    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

    Insert

    Lyn-dInPAkt
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase, dd…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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