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pcDNA3.1+ Flag-RAP2A

Plasmid

#128099

Purpose

Expresses RAP2A in mammalian cells in a G418-selectable plasmid

Depositor

Chi Van Dang

Article

Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

Insert

RAP2A (RAP2A Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-H2B-tdTomato-SRT

Plasmid

#203393

Purpose

AAV transfer plasmid containing H2B fused piggyBac self-reporting transposon with tdTomato marker for mammalian calling cards

Depositor

Joseph Dougherty, Rob Mitra

Article

Yen et al Curr Protoc. 2023 Sep;3(9):e883. doi: 10

Insert

H2B fused piggyBac tdTomato self-reporting transposon

Use

AAV

Tags

3X SV40 and H2B

Expression

Mammalian

Promoter

Ef1a

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast FLVCR2

Plasmid

#218537

Purpose

human FLVCR2 cDNA

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

FLVCR2 (FLVCR2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast FLVCR1b

Plasmid

#218535

Purpose

human FLVCR1b cDNA

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

FLVCR1 (FLVCR1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Alternative isoform

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast FLVCR1/FLVCR1a

Plasmid

#218533

Purpose

human FLVCR1/FLVCR1a cDNA

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

FLVCR1 (FLVCR1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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CD3-2A_pMI-LO

Plasmid

#153418

Purpose

Retroviral (MSCV) expression of all four WT CD3 subunits, co-expressed with LSSmOrange

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

murine CD3 delta-gamma-epsilon-zeta subunits (Cd3d Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC25A26

Plasmid

#132259

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A26 (SLC25A26 Human)

Expression

Mammalian

Available Since

Nov. 19, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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Sec61β-Flag-APEX2-SOPP3

Plasmid

#229072

Purpose

Expresse APEX2-SOPP3 in ER in mammalian cell

Depositor

Weijun Pan

Article

Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

Insert

Express APEX2-SOPP3 in the ER (APEX2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS 3xFLAG Mbnl2

Plasmid

#217032

Purpose

Overexpression of Mbnl2 in mammalian cells

Depositor

Chaolin Zhang

Article

Weyn-Vanhentenryck et al Nat Commun. 2018 Jun 6;9(1):2189. doi: 1

Insert

Mbnl2 (Mbnl2 Mouse)

Expression

Mammalian

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC5A2

Plasmid

#132241

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC5A2 (SLC5A2 Human)

Expression

Mammalian

Available Since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC6A14

Plasmid

#131865

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC6A14 (SLC6A14 Human)

Expression

Mammalian

Available Since

Oct. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC2A2

Plasmid

#132126

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC2A2 (SLC2A2 Human)

Expression

Mammalian

Available Since

Dec. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pAS_4xG1 PylT FLAG-G1 PylRS Y125A

Plasmid

#154769

Purpose

plasmid with 4xG1 PylT cassette and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)

Tags

FLAG

Expression

Mammalian

Mutation

Y125A

Promoter

EF1

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pLVNP3.0-PEmaxRH

Plasmid

#206884

Purpose

Expresses FLAG-tagged PEmax (lacking the RNAseH domain) fused to Gag through a linker sequence

Depositor

Jacob Giehm Mikkelsen

Article

Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

Insert

PEMax Delta RNAseH

Tags

FLAG

Promoter

CMV

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLVNP3.0-ABE8e

Plasmid

#206882

Purpose

Expresses FLAG-tagged ABE8e fused to Gag through a linker sequence

Depositor

Jacob Giehm Mikkelsen

Article

Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

Insert

ABE8e

Use

CRISPR and Lentiviral

Tags

FLAG

Promoter

CMV

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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PGK mScarlet-LC3B

Plasmid

#200083

Purpose

Mammalian expression of fluorescently-tagged marker for autophagic vesicles

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

Map1lc3b (Map1lc3b Rat)

Tags

mScarlet

Expression

Mammalian

Promoter

PGK

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-LTK-V5/HIS

Plasmid

#200116

Purpose

Expression of human LTK receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human LTK receptor tyrosine kinase (LTK Human)

Tags

V5/His

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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AID∆C-Cas9n-UGI-NLS

Plasmid

#198890

Purpose

Express AID(1-188) BE3-based base editor

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

Actication induced deaminase (AICDA Human)

Tags

NLS

Expression

Mammalian

Mutation

Deletion of amino acids 189-198

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-CERT(PHD)

Plasmid

#221746

Purpose

Stable expression of GFP-CERT(PHD)in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

CERT (CERT1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

amino acids 1–116

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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hSyn-eGFP-DDHD2

Plasmid

#232463

Purpose

Expresses GFP tagged DDHD2 lipase in neurons

Depositor

Timothy Ryan

Article

Kumar et al Nat Metab. 2025 Jul;7(7):1392-1403. doi:

Insert

DDHD2 (DDHD2 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Promoter

hSyn

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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