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Showing: 2051 - 2100 of 2321 results

  1. PC1575

    Plasmid
    #232088
    Purpose
    Mammalian expression plasmid for ubiquibody (uAb) cloning backbone. Includes an Esp3I restriction site directly upstream of GSGSG linker and CHIPΔTPR CDS and a C-terminal IRES-mCherry cassette.
    Depositor
    Pranam Chatterjee
    Article
    Bhat et al bioRxiv [Preprint]. 2024 Jul 22:2023.06.
    Insert
    uAb with cloning Esp3I restriction site
    Use
    Tags
    IRES-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    232088-plasmid-map-sequence-id-464073

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  24. pDGB3_alpha1 nptII-DISTC02-CBS-FRT-Rep-RepA-tNos-DISTC19-CBS-miniDFR-FLP-tNos-PROXC02-pNos-CUP2-Gal4AD-tNos-SF (GB4697)

    Plasmid
    #225440
    Purpose
    Module for copper and flippase-regulated expression of BeYDV Rep/RepA, including the TU for copper-regulated FLP, the TU for constitutive expression of CUP2-GAL4, and the nptII cassette.
    Depositor
    Diego Orzaez
    Insert
    nptII-DISTC02-CBS-FRT:Rep/RepA:tNos-DISTC19-CBS:miniDFR:FLP:tNos-PROXC02-pNos:CUP2:Gal4AD:tNos-SF
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225440-plasmid-map-sequence-id-447732

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  32. YW347_Ptbb2UTR-loxP-RFP-miniMOS

    Plasmid
    #196064
    Purpose
    a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of myo2p::TagRFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and tagRFP cassettes.
    Depositor
    Wenxing Yang
    Article
    Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77
    Inserts
    myo2p driven tagRFP
    NeoR
    Minimal Mos1
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    myo-2
    Available since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    196064-plasmid-map-sequence-id-382980

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  33. pTS2361_Tier3(SB)-rtTA-YPet_PuroR

    Plasmid
    #169657
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRPBSA
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169657-plasmid-map-sequence-id-365632

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  34. pTS2363_Tier3(PB)-rtTA-YPet_PuroR

    Plasmid
    #169659
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRPBSA
    Available since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    169659-plasmid-map-sequence-id-364557

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  35. pBY011-AMN1ko

    Plasmid
    #183099
    Purpose
    S. cerevisiae Sigma1278b AMN1 gene knock out plasmid with KanMX selection marker.
    Depositor
    Gabor Balazsi
    Article
    Guinn et al Commun Biol. 2022 Mar 31;5(1):292. doi:
    Insert
    AMN1 (left homology arm) - KanMX - AMN1 (right homology arm) (AMN1 Budding Yeast)
    Use
    Tags
    KanMX
    Expression
    Bacterial and Yeast
    Mutation
    only includes external sequences (504 bp each) as…
    Promoter
    URA3, TEF1
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183099-plasmid-map-sequence-id-361230

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  37. VHACg/pDONR221

    Plasmid
    #172094
    Purpose
    Genomic fragment containing the upstream region and coding region of DET3/VHA-C cloned into pDONR221
    Depositor
    George Haughn
    Article
    Shi et al Plant Physiol. 2019 Nov;181(3):901-915.
    Insert
    VHA-C genomic region including 530bp upstream of the ATG, exons, introns, and no stop codon (DET3 Mustard Weed)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    530bp upstream of VHA-C ATG
    Available since
    Oct. 20, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    172094-plasmid-map-sequence-id-345740

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  44. FLAG-LoxP-GFP-LoxP-SNAP-TERT

    Plasmid
    #71391
    Purpose
    Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes GFP marker flanked by LoxP sites for selection.
    Depositor
    Thomas Cech
    Article
    Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:
    Insert
    TERT (TERT Human)
    Use
    Hr donor
    Tags
    FLAG-SNAP
    Expression
    Mutation
    Promoter
    Endogenous TERT promotor
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    71391-plasmid-map-sequence-id-128452

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  45. pSicoR-Ef1a-mCh-Puro

    Plasmid
    #31845
    Purpose
    Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression. Includes puromycin selection.
    Depositor
    Bruce Conklin
    Article
    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 for shRNA; Ef1alpha for mCherry-puromycin
    Available since
    Jan. 3, 2012
    Availability
    Academic Institutions and Nonprofits only
    31845-plasmid-map-sequence-id-194345

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  46. pAAV_GR-RE_MLPmin_BC1092-luc2

    Plasmid
    #227112
    Purpose
    Glucocorticoid receptor response element for luciferase and barcode assays; barcode BC1092
    Depositor
    Michael Wehr
    Article
    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523
    Insert
    12x clustered GR response element linked to MLP minimal promoter driving barcode 1092 and a luciferase reporter gene
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227112-plasmid-map-sequence-id-456025

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  48. pUT_mNF-Cas13d-Triplex

    Plasmid
    #227709
    Purpose
    LPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Include a 3' UTR Triplex motif as a template backbone for MONARCH 2.0 system.
    Depositor
    Gabor Balazsi
    Article
    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-
    Insert
    mCherry-P2A-RfxCas13d-Triplex
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-d2i
    Available since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    227709-plasmid-map-sequence-id-450930

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  49. MmEos3.2

    Plasmid
    #203754
    Purpose
    Encodes the membrane anchor of Src including the first 15 resideues of the N terminus, with 1 myristoylation and short polybasic sequence, with mEos3.2 fused to the C terminus. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    Src15 n-term (SRC Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    203754-plasmid-map-sequence-id-403344

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Showing: 2051 - 2100 of 2321 results