175,318 results

Showing: 8421 - 8440 of 175318 results

Results per page:

Sort results by:

pLV hUbC-dCas9 KRAB-T2A-GFP

Plasmid

#67620

Purpose

Co-expressed human optimized S. pyogenese dCas9 fused to KRAB repressor domain and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized dead Cas9 KRAB T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Mutation

D10A and H840A

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.22-VEGF-HRE::dLUC

Plasmid

#128096

Purpose

Hypoxia response element real-time luciferase reporter

Depositor

Chi Van Dang

Article

Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

Insert

Hypoxia response element (x5) from VEGF promoter (VEGFA Human)

Use

Luciferase

Tags

drives expression of destabilized luciferase (2CP…

Expression

Mammalian

Promoter

inserted VEGF hypoxia response elements (HRE) (x5)

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLGB31

Plasmid

#126621

Purpose

Suicide vector for allelic replacement in Bacteroides, inulin selection and rhamnose-inducible ss-Bfe1 counterselection

Depositor

Laurie Comstock

Article

Garcia-Bayona et al mBio. 2019 Aug 13;10(4). pii: mBio.01762

Type

Empty backbone

Use

Allellic exchange in bacteria

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS-Akt-FoxO3a-KTR-EGFP

Plasmid

#125131

Purpose

Fluorescent reporter for Akt activity

Depositor

Kazuhiro Aoki

Article

Maryu et al Cell Struct Funct. 2016 Jul 22;41(2):81-

Insert

FOXO2 (FOXO3 Human)

Tags

Enhanced green fluorescent protein (EGFP)

Expression

Mammalian

Mutation

FoxO3A 1-402 amino acid

Promoter

CAG promoter

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMGS54 (AID-P2A-Hygromycin)

Plasmid

#126583

Purpose

An AID-P2A-HygR cassette to tag the protein of interests with auxin-inducible degron at the c-terminal

Depositor

Michael Guertin

Article

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

AID-P2A-HygR

Use

Other

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
epB_UbC_CymRV5-nls-GFP_DEx2

Plasmid

#119906

Purpose

ePiggyBac mammalian expression plasmid for CymR-GFP fusion

Depositor

Orion Weiner

Article

Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

Insert

CymRV5-nls-GFP

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
plenti-ATF4

Plasmid

#125238

Purpose

ATF4 expression vector

Depositor

Rozita Yarmand

Article

Bagheri-Yarmand et al J Biol Chem. 2015 May 1;290(18):11749-61

Insert

ATF4 (ATF4 Human)

Use

Lentiviral

Tags

Flag

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE60-EFTs-His

Plasmid

#124128

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Elongation factor Ts (tsf Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE30-His-AsnRS

Plasmid

#124105

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Asparaginyl-tRNA ligase (asnS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a-AspRS-His

Plasmid

#124106

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Aspartate-tRNA ligase (aspS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a-ValRS-His

Plasmid

#124122

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Valyl-tRNA ligase (valS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-BRD4ΔN-mCherry-sspB

Plasmid

#121968

Purpose

Expresses fusion of disordered protein BRD4(462-1362), fluorescent protein mCherry, and sspB which upon light activation binds to iLID.

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

BRD4 (BRD4 Human)

Use

Lentiviral

Tags

mCherry-sspB

Expression

Mammalian

Mutation

Deleted amino acids 1-461

Promoter

SFFV

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29b-H6_Streptavidin_sfGFP

Plasmid

#124296

Purpose

His6-tagged Streptavidin GFP fusion protein

Depositor

Mark Arbing

Insert

His6 tag - TEV protease site - T7 tag - Streptavidin (15-159) - superfolder GFP

Tags

His6 and T7 tags

Expression

Bacterial

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK345 (Hygro-P2A-mAID-mClover)

Plasmid

#121179

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Hygro-P2A-mAID-mClover

Use

CRISPR

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLH-sgRNA-Sirius-8XMS2

Plasmid

#121939

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-8XMS2

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

human U6 promoter

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-HuR

Plasmid

#121162

Purpose

Mammalian expression of GFP tagged HuR

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

HuR (ELAVL1 )

Expression

Mammalian

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-TRE-DIO-FLPo

Plasmid

#118027

Purpose

AAV vector for sparse and bright labeling in a cell-type specific manner

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

Insert

TRE-DIO-FLPo

Use

AAV

Promoter

TRE

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti6-CENP-A-mCherry

Plasmid

#89767

Purpose

Lentivirus destination vector for stable expression of mCherry-tagged kinetochore marker CENP-A

Depositor

Torsten Wittmann

Article

Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

Insert

CENP-A (CENPA Human)

Use

Lentiviral

Tags

3xHA and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBH545_Tet-Cas14a1_Locus

Plasmid

#112501

Purpose

Expresses Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14a1

Use

CRISPR

Expression

Bacterial

Promoter

Tet

Available Since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM1 Raptor GFP

Plasmid

#112744

Purpose

express Raptor-GFP

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Raptor-GFP (RPTOR Human)

Use

Lentiviral

Tags

eGFP

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge