165,537 results

1. pEff

   Plasmid

   #97423

   Purpose

   Transient expression of recombinant proteins in plants. The plasmid contains gfp, which is replaced by the gene of interest.

   Depositor

   Nikolai Ravin

   Article

   Mardanova et al Front Plant Sci. 2017 Feb 28;8:247. doi:

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   July 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV_2pabPAC

   Plasmid

   #158969

   Purpose

   2P-activatable bPAC for AAV

   Depositor

   Michiyuki Matsuda

   Article

   Kinjo et al ACS Chem Biol. 2020 Oct 19. doi: 10.1021

   Insert

   2pabPAC
   

   Use

   AAV

   Promoter

   CMV

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. TARS_pNIC-Bio3

   Plasmid

   #153057

   Purpose

   Expression construct for producing Avi tagged Threonyl-tRNA synthetase

   Depositor

   Susanne Gräslund

   Insert

   TARS (TARS1 Human)
   

   Tags

   Avi and His6-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry-LSS-SGFP2

   Plasmid

   #112943

   Purpose

   Produces a fusion between LSS-GFP2 and mCherry. It can be used as a positive control for FRET

   Depositor

   Dorus Gadella

   Insert

   LSS-GFP2 mCherry fusion
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5-myc-Cdc42-wt

   Plasmid

   #12972

   Depositor

   Gary Bokoch

   Insert

   Cdc42 (CDC42 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAK005

   Plasmid

   #233642

   Purpose

   For bacterial expression of eSpCas9(1.1) c-terminally tagged with VitelloTagB.

   Depositor

   Zak Swartz

   Article

   Clarke et al Development. 2024 Oct 15;151(20):dev2028

   Insert

   eSpCas9(1.1) with c-terminal VitelloTagB
   

   Tags

   6-His

   Expression

   Mammalian

   Available Since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTRANS_253

   Plasmid

   #91125

   Purpose

   Transformation, Type: T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: WDV

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   35S

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)

   Plasmid

   #140003

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. TEV Protease-mCherry

   Plasmid

   #243793

   Purpose

   Encodes for mammalian expression of TEV protease input. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.

   Depositor

   Cole DeForest

   Article

   Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

   Insert

   TEV protease
   

   Expression

   Mammalian

   Available Since

   Oct. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. TEV-HRV-3C Proteases-mCherry

    Plasmid

    #243795

    Purpose

    Encodes for mammalian expression of TEV and HRV-3C protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.

    Depositor

    Cole DeForest

    Article

    Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

    Insert

    TEV and HRV-3C proteases
    

    Expression

    Mammalian

    Available Since

    Oct. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti6.3-CD300C-V5

    Plasmid

    #124089

    Purpose

    CD300C-V5 tagged expression vector

    Depositor

    J. Chad Brenner

    Article

    Mann et al Oral Oncol. 2023 Nov;146:106562. doi: 10

    Insert

    CD300C (CD300C Human)
    

    Use

    Lentiviral

    Tags

    V5

    Available Since

    Oct. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. L4802 VEGFR2 NTEVp chain (WT NTEVp) in PolyTX-mNeonGreen

    Plasmid

    #244173

    Purpose

    Constitutive expression of a MESA NTEVp chain encoding (N to C): VEGFR2SS-3xFLAG-VEGFR2ECD-VEGFR2TMD-NTEVp(WT)

    Depositor

    Joshua Leonard

    Article

    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

    Insert

    MESA NTEVp chain with human VEGFR2 SS, ECD and TMD, and NTEVp (WT) (KDR Human, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    VEGFR2 signal peptide - 3xFLAG

    Expression

    Mammalian

    Promoter

    hEF1a

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. Split PS Intein tTA

    Plasmid

    #242028

    Purpose

    Blue-light-controlled nuclear translocation of split tTA transactivator from the cytoplasm. Split version.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

    Insert

    Split PS Intein tTA
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAttBFHyg2

    Plasmid

    #244927

    Purpose

    attB Hyg Resistant plasmid for RMI targeting an attP site

    Depositor

    Michael Nonet

    Article

    Nonet et al bioRxiv 2024.03.01.583017

    Type

    Empty backbone

    Use

    Phic31 recombinase mediated insertion vector

    Expression

    Worm

    Promoter

    none

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. QBEmax

    Plasmid

    #238001

    Purpose

    Expresses the QBEmax base editor in mammalian cells

    Depositor

    Kevin Zhao

    Article

    Hu et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    QBEmax base editor
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. IRF3-V5

    Plasmid

    #169775

    Purpose

    Express IRF3 in mammalian cells

    Depositor

    Sung-Liang Yu

    Article

    Chang et al Commun Biol. 2021 Jun 2;4(1):663. doi: 1

    Insert

    Homo sapiens interferon regulatory factor 3 (IRF3), transcript variant 1 (IRF3 Human)
    

    Tags

    V5 tag

    Expression

    Bacterial and Mammalian

    Available Since

    July 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Tad-CBE3.6

    Plasmid

    #235058

    Purpose

    Expresses Tad-CBE3.6 in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE3.6-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, SV40NLS and bpNLS

    Expression

    Mammalian

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET29b-Rhobin2

    Plasmid

    #239018

    Purpose

    Bacterial overexpression of de novo Rhodamine binder 2 (Rhobin2) protein

    Depositor

    Bo Huang, William DeGrado

    Article

    Chen et al bioRxiv 2025.06.24.661379

    Insert

    RhoBin2
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMEDS

    Plasmid

    #236568

    Purpose

    Introduces a multiple expression and dual selection (MEDS) system to use alongside CRISPR for applications such as gene editing.

    Depositor

    Shannon Moonah

    Article

    Sharma et al PLoS One. 2025 May 6;20(5):e0321881. doi

    Inserts

    CD/UPRT (negative selection)
    NPT II (positive selection)
    

    Tags

    3x Myc and FLAG

    Expression

    Mammalian

    Available Since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLCKO-CMV-TMEM106B-P2A-Blasticidin

    Plasmid

    #237478

    Purpose

    Encodes full length human TMEM106B protein, along with blasticidin selection marker.

    Depositor

    Dirk Daelemans

    Article

    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.

    Insert

    TMEM106B (TMEM106B Human)
    

    Use

    Lentiviral

    Tags

    P2A-Blasticidin

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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