159,557 results

1. Fuw-dCas9-Tet1CD-P2A-BFP

   Plasmid

   #108245

   Purpose

   Lentiviral vector to express dCas9-Tet1CD-P2A-tagBFP

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

   Insert

   dCas9-Tet1CD-HA-P2A-BFP
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   dCas9-D10A, dCas9-H840A

   Promoter

   Ubc

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. SERPINE2-bio-His

   Plasmid

   #53416

   Purpose

   Expresses full-length Glia-derived nexin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   SERPINE2 (SERPINE2 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 8522-M01-663

   Plasmid

   #225660

   Purpose

   Lentiviral expression of a cancer stem cell reporter that responds to presence of Sox2/Oct4 or their paralogs (green configuration)

   Depositor

   Dominic Esposito

   Article

   Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

   Insert

   dsCopGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SORE6-mCMVp

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. paavCAG-post-mGRASP-2A-dTomato

   Plasmid

   #34912

   Purpose

   post

   Depositor

   Jinhyun Kim

   Article

   Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

   Insert

   postsynaptic mGRASP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Nov. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG-Mkk7-JNK2 fusion-pcw107

   Plasmid

   #64618

   Purpose

   Mkk7-JNK2 fusion - barcoded activating construct of JNK pathway

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   Map2k7 linker MAPK9
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   mouse-human fusion

   Promoter

   PGK

   Available since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pCR4-myco

   Plasmid

   #36872

   Depositor

   Mario Chin

   Insert

   myco
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV-GFP-CaM-uTEVp

   Plasmid

   #163028

   Purpose

   FLiCRE protease for HEK293T expression

   Depositor

   Alice Ting

   Article

   Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

   Insert

   GFP-CaM-uTEVp
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pH2B-Electra2

   Plasmid

   #179479

   Purpose

   Nuclear labeling with blue fluorescent protein Electra2 fused to H2B

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   H2B-Electra2
   

   Use

   Tags

   Electra2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEVOL-pylT-AznLRS

   Plasmid

   #172482

   Purpose

   For E.Coli expression, 306L309L348I384F411W

   Depositor

   Wenshe Liu

   Article

   Wang et al Angew Chem Int Ed Engl. 2017 Feb 1;56(6)

   Insert

   AznLRS and pylTcua
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   July 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GLuc-FLAG-CB1

    Plasmid

    #158066

    Purpose

    Membrane expression detection of Cannabinoid receptor 1

    Depositor

    Adolfo Rivero-Muller

    Article

    Rodriguez-Rodriguez et al Sci Rep. 2020 Oct 23;10(1):18191. doi: 1

    Insert

    CB1 (CNR1 Human)
    

    Use

    AAV

    Tags

    Gaussia Luciferase

    Expression

    Mammalian

    Mutation

    contains 4Kb of 3' UTR

    Promoter

    pCAG

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. iF59 PB-TO-oNgn2-puro

    Plasmid

    #204734

    Purpose

    Improved Dox-inducible neurogenin-2; Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase.

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Optimized neurogenin-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEF-BFP

    Plasmid

    #138272

    Purpose

    Expression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editor

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

    Insert

    BFP H66 PAM
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    H66, PAM motif

    Promoter

    Ef1a

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TFORF0060

    Plasmid

    #141501

    Purpose

    Lentiviral vector for overexpressing the CTBP2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CTBP2 (CTBP2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRRL-MHCK7-GCaMP6

    Plasmid

    #65042

    Purpose

    Lentiviral vector expressing the calcium indicator GCaMP6 driven by the muscle-specific promoter MHCK7

    Depositor

    Nenad Bursac

    Article

    Madden et al eLife. 2015 Jan 9;4:e04885. doi: 10.7554

    Insert

    GCaMP6
    

    Use

    Lentiviral

    Tags

    6xHis, T7 tag, and Xpress epitope

    Expression

    Mammalian

    Mutation

    Promoter

    MHCK7

    Available since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG36

    Plasmid

    #35126

    Depositor

    John McCusker

    Article

    Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

    Insert

    nat1
    

    Use

    Yeast replicating

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-iCas9

    Plasmid

    #160048

    Purpose

    inducible expression of Cas9

    Depositor

    Xiaojun Lian

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCRISPaint-mNeon

    Plasmid

    #174092

    Purpose

    CRISPaint gene tagging

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    mNeonGreen
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKH3-TRIM28

    Plasmid

    #45569

    Depositor

    Fanxiu Zhu

    Article

    Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

    Insert

    TRIM28 (TRIM28 Human)
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 29, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pEGFPC1-hVAP-B

    Plasmid

    #104448

    Purpose

    Expression of human VAP-B fused to GFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

    Insert

    vesicle-associated membrane protein-associated protein B (VAPB Human)
    

    Use

    Tags

    EGFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP-N1-FUS/TLS-FLAGC

    Plasmid

    #60362

    Purpose

    Plasmid for expression of FLAG-GFP tagged human FUS/TLS (C-terminal tag). Confers resistance to G418.

    Depositor

    Patrick Calsou, Sébastien Britton

    Article

    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge