We narrowed to 11,803 results for: nsf

1. pAAV Syn ChR E90R-D156N-T159C 2A tDimer

   Plasmid

   #52494

   Purpose

   Historical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)

   Depositor

   Thomas Oertner

   Article

   Wietek et al Science. 2014 Mar 27.

   Inserts

   Channelrhodopsin-2
   red fluorescent protein
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   E90R, D156N, T159C

   Promoter

   human synapsin-1 and ribosomal skip sequence

   Available Since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-EF1a-FRT-JEDI-1P-Kv-WPRE

   Plasmid

   #202617

   Purpose

   Genetically encoded voltage indicator (GEVI) JEDI-1P flanked by FRT in AAV production vector expressed under the mammalian promoter (EF1a), FLP recombinase-dependent

   Depositor

   Francois St-Pierre

   Article

   Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

   Insert

   FRT-JEDI-1P-Kv
   

   Use

   AAV; Flp/frt

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-CMV-SspB-EGFP-VP16-P2A-FLAG-Gal4DBD-CapN-SsrA-CapC-HA

   Plasmid

   #213536

   Purpose

   Expresses the split transcription factors in mouse liver

   Depositor

   Wenjing Wang

   Article

   Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

   Insert

   SspB-EGFP-VP16-P2A-FLAG-Gal4DBD-CapN-SsrA-CapC-HA-HA
   

   Use

   AAV

   Tags

   EGFP and HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-CaMKII-ASAP2s

   Plasmid

   #101275

   Purpose

   Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for expression in excitatory glutamatergic neurons using the promoter CaMKII

   Depositor

   Francois St-Pierre

   Article

   Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

   Insert

   ASAP2s
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAMKIIa

   Available Since

   Nov. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AAV CMV-driven AcrIIA4-scaffold (2xBsmBI sites)

   Plasmid

   #120297

   Purpose

   AAV vector for expression of AcrIIA4 (no miR binding sites, control vector)

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   AcrIIA4
   

   Use

   AAV and CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1(+) M-Ras(WT)

   Plasmid

   #224287

   Purpose

   Cell transfection and expression of M-Ras

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   M-Ras (MRAS Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-Ef1a-DO-ChETA-TdTomato-WPRE-pA

   Plasmid

   #37756

   Purpose

   Expresses ChETA-TdTomato in cells lacking Cre (Cre-Off), for optogenetic excitation

   Depositor

   Bernardo Sabatini

   Article

   Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

   Insert

   ChETA-TdTomato
   

   Use

   AAV and Cre/Lox; Cre-off

   Tags

   TdTomato

   Promoter

   EF1a

   Available Since

   July 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p3XFlag-Mettl4

   Plasmid

   #86665

   Purpose

   Expresses murine Mettl4 containing N-terminal 3XFlag tag

   Depositor

   Douglas Feldman

   Article

   Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

   Insert

   Mettl4 (Mettl4 Mouse)
   

   Tags

   3xFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-DIO-mDLX-TVA-2A-N2cG

   Plasmid

   #172365

   Purpose

   Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from cre-expressing inhibitory neurons

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   TVA + B19-N2cG
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   mDLX

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEJS2603_AAV-Nme2-ABE8e-i1-U6-Rosa26

    Plasmid

    #201685

    Purpose

    All-in-one AAV plasmid expressing Nme2-ABE8e-i1 and U6 driven sgRNA targeting the mouse Rosa26 gene

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

    Insert

    Nme2-ABE8e-i1
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    U1a

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-FDIO-IGK-dAPEX2-KDEL

    Plasmid

    #117184

    Purpose

    Plasmid name in publication: pAAV-FDIO-ER-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    IGK-dAPEX2-KDEL
    

    Use

    AAV; Flp/frt

    Expression

    Mammalian

    Mutation

    W41F and A134P on soybean APX

    Promoter

    EF1a

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MSP3-bio

    Plasmid

    #47731

    Purpose

    Expresses enzymatically monobiotinylated full-length MSP3 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised MSP3
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-CAG-soCoChR-GFP

    Plasmid

    #107710

    Purpose

    A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. CAG promoter

    Depositor

    Edward Boyden

    Article

    Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

    Insert

    soCoChR-GFP
    

    Use

    AAV

    Tags

    EGFP and KA2

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-FDIO-LACTB-dAPEX2

    Plasmid

    #117181

    Purpose

    Plasmid name in publication: pAAV-FDIO-IMS-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    LACTB-dAPEX2
    

    Use

    AAV; Flp/frt

    Expression

    Mammalian

    Mutation

    W41F and A134P on soybean APX

    Promoter

    EF1a

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MSP2-bio

    Plasmid

    #47710

    Purpose

    Expresses enzymatically monobiotinylated full-length MSP2 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised MSP2
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-EF1a-Flex-PAK1-AID-ires-EGFP

    Plasmid

    #83489

    Purpose

    cre-dependent PAK1-AID under EF1a promoter

    Depositor

    Dong Kong

    Article

    Kong et al Neuron. 2016 Jun 16. pii: S0896-6273(16)

    Insert

    PAK1-AID-ires-EGFP
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLV-REPORT(PGK)

    Plasmid

    #172328

    Purpose

    LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an PGK promoter driving nuclear d2eGFP E2A Hygromycin

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    d2eGFP
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3x SV40 NLS

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TS-PE2-N

    Plasmid

    #176649

    Purpose

    Expresses N-terminus of PE2 in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

    Inserts

    N-terminus of PE2
    Artificial SD
    

    Use

    AAV

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV and None

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-CaMKII-pAceR-Kv2.1PR

    Plasmid

    #195531

    Purpose

    Red fluorescent, positive response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    pAceR-Kv2.1 proximal restriction sequence
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    VARNAM 78E, 81D, 92N; SI linker

    Promoter

    CaMKII

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAT-mCherry2-G

    Plasmid

    #164169

    Purpose

    Expresses mCherry2 under the control of TetO promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal, pAAV-TRE backbone

    Depositor

    Isei Tanida

    Article

    Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

    Insert

    mCherry2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    tetO

    Available Since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge