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pLentiRNACRISPR_003 - hU6-DR_BsmBI-EFS-PspCas13b-NLS-2A-Puro-WPRE

Plasmid

#138145

Purpose

Expresses PspCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI.

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

PspCas13b

Use

Lentiviral

Tags

NLS

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-Skp2

Plasmid

#89142

Purpose

mammalian expression of human Skp2 with N-terminal FLAG tag

Depositor

Elizabeth Wilson

Article

Su et al Mol Cell Endocrinol. 2017 Jan 5;439:1-9.

Insert

Skp2 (SKP2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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pYFAC-CH2-ubi-M-pyrG

Plasmid

#221132

Purpose

Expression of heterologous genes under alcohol induction optimised with ubi-M-pyrG marker. Promoters PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction sites: PacI, NotI, AsiSI, AscI.

Depositor

Yit Heng Chooi

Article

Roux et al Microb Biotechnol. 2024 Sep;17(9):e70010

Type

Empty backbone

Use

Synthetic Biology; Expression in aspergillus and …

Promoter

PalcA-T1-PalcS/M-T2-PaldA expression cassette (al…

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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AALN-BE4max

Plasmid

#138161

Purpose

C-to-T base editor

Depositor

David Liu

Article

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

rAPOBEC1(AALN)-nSpCas9-UGI-UGI

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 R33A + K34A + H122L + D124N; with…

Promoter

CMV

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-Paxillin

Plasmid

#176107

Purpose

Focal Adhesion marker

Depositor

Dorus Gadella

Article

Arts et al Front Immunol. 2021 May 18;12:667213. do

Insert

Paxillin (PXN Human)

Use

Lentiviral

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pSC50

Plasmid

#104824

Purpose

CRISPR/Cas9 2xplex gRNA targeting Glyma.08g081600, Glyma.05g126600 (Hen1ab). Also expresses Cas9 from Gmubi promoter.

Depositor

Robert Stupar

Article

Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

Insert

Glyma.08g081600, Glyma.05g126600

Use

CRISPR

Expression

Plant

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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xCas9

Plasmid

#138565

Purpose

Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

xCas9

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Promoter

EFS

Available Since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pNICKclos2.0

Plasmid

#73228

Purpose

Genome editing for gene xylR (cbei-2385) in clostridium beijerinckii NCIMB 8052

Depositor

Sheng Yang

Article

Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

Inserts

Cas9 nickase
sgRNA to xylR

Use

E.coli - clostridium shuttle vector

Mutation

D10A

Promoter

Pj23119 and Pthl

Available Since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-SUMO-MjDim1

Plasmid

#189936

Purpose

Expression of Mjdim1, a methyltransferase used in m6A-SAC-seq.

Depositor

Chuan He

Article

Hu et al Nat Biotechnol. 2022 Mar 14. pii: 10.103

Insert

rsmA (MJ_RS00455 Methanocaldococcus jannaschii)

Tags

6x His tag, FLAG tag, SUMO tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pHT-6xR.ads-PxylA-DI

Plasmid

#47386

Purpose

Expresses ads under Pgrac promoter (inducible by IPTG) and expresses dxs and idi under PxylA promoter (inducible by xylose) in bacteria

Depositor

Heng-Phon Too

Article

Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

Inserts

ads
dxs
idi

Tags

6xR and His

Expression

Bacterial

Promoter

Pgrac and PxylA

Available Since

Oct. 10, 2013

Availability

Academic Institutions and Nonprofits only

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EGFP gRNA (BRDN0000561167)

Plasmid

#80034

Purpose

3rd generation lentiviral gRNA plasmid targeting EGFP

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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gCH29 (crCD81-1)

Plasmid

#217344

Purpose

crRNA targeting CD81

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

crCD81-1 (CD81 Human)

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDarmo.CMVT_v1

Plasmid

#133072

Purpose

Entry vector for cloning genes under an enhanced CMVT promoter for expression in mammalian cells. Version 1.0.

Depositor

Kacper Rogala, David Sabatini

Article

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Entry vector

Tags

3C-Flag-TwinStrep and TwinStrep-Flag-3C

Expression

Mammalian

Promoter

CMVT (Enhanced CMV)

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pHLuc (Antares-SEPLuc C1A4E+6)

Plasmid

#141089

Purpose

final optimized bioluminescent reporter for detecting extracellular pH changes in tumor acidosis

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

Antares-T2A-puromycin-IRESv24-SEP-C1A4E+6

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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scaffold-linker-U6

Plasmid

#89639

Purpose

construction of paired sgRNAs driven by two U6 promoters. Vector itself only has one U6 promoter.

Depositor

Wensheng Wei

Article

Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pSL3936 (pDonor(AAVS1)_Pse)

Plasmid

#200903

Purpose

Encodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a SA-T2A-PuroR-T2A-eGFP cargo. Total transposon size = 2,024 bp.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

Mini Tn7016 (AAVS1 NGS)

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Target-AIDmax

Plasmid

#139107

Purpose

Mammalian Target-AIDmax expression vector (pSI-626)

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Bi_partite_NLS-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAB1679 CMV-HIVNES-GS-Cas13bt3

Plasmid

#176317

Purpose

CMV-HIVNES-GS-Cas13bt3 (human codon optimized Cas13bt3 expression)

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

Insert

human codon optimized Cas13bt3

Use

CRISPR

Tags

HIV NES

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pF420

Plasmid

#123659

Purpose

Produces cofactor F420 in E. coli

Depositor

Colin Jackson

Article

Bashiri et al Nat Commun. 2019 Apr 5;10(1):1558. doi:

Inserts

CofD
FbiB
FbiD
FbiC

Use

Synthetic Biology

Expression

Bacterial

Promoter

BBa_R0040

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-sgRNA(lib)-puro

Plasmid

#119976

Purpose

Lenti sgRNA cloning backbone with CMV-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Wensheng Wei

Article

Liu et al Nat Biotechnol. 2018 Nov 5. pii: nbt.428

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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