We narrowed to 11,847 results for: NSI

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pGAL4-DBD-HOXA13-44A-IDR

Plasmid

#145237

Purpose

Expresses fusion of GAL4 DNA-binding domain and HOXA13-44A IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXA13-44A-IDR (HOXA13 Human)

Expression

Mammalian

Mutation

A-repeats deleted

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXA13-15A-IDR

Plasmid

#145236

Purpose

Expresses fusion of GAL4 DNA-binding domain and HOXA13-15A IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXA13-15A-IDR (HOXA13 Human)

Expression

Mammalian

Mutation

A-repeats deleted

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HEY2-Adel-IDR

Plasmid

#145234

Purpose

Expresses fusion of GAL4 DNA-binding domain and HEY2-Adel IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HEY2-Adel-IDR (HEY2 Human)

Expression

Mammalian

Mutation

A-repeats deleted

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-BHLHE41-Adel-IDR

Plasmid

#145228

Purpose

Expresses fusion of GAL4 DNA-binding domain and BHLHE41-Adel IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

BHLHE41-Adel-IDR (BHLHE41 Human)

Expression

Mammalian

Mutation

A-repeats deleted

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA8-Flag-LARS(1-720aa)

Plasmid

#139687

Purpose

Expresses N-teminal Flag tagged aa 1- 720 of LARS1

Depositor

Jie Chen

Article

Yoon et al Cell Rep. 2016 Aug 9;16(6):1510-1517. do

Insert

LARS1 (LARS1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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pSB61 - pL0_V2 (CDS1)

Plasmid

#123183

Purpose

Golden Gate (MoClo; CDS1) compatible Tomato yellow leaf curl virus (TYLCV) V2 silencing suppressor based on NCBI Reference Sequence NC_004005

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

Tomato yellow leaf curl virus (TYLCV) V2 (v2 Synthetic)

Use

Part for plant expression

Expression

Plant

Mutation

No BpiI and BsaI sites

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-FLEX-rc [ArchT-tdTomato]

Plasmid

#123607

Purpose

AAV mediated expression of ArchT-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. tdTomato has codons varied to reduce recombination. Using bGHpA signal.

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Insert

ArchT-tdTomato

Use

AAV

Tags

tdTomato

Expression

Mammalian

Promoter

Syn

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLL5 sgRNA(MS2)-MS2-hA3a D131A

Plasmid

#112131

Purpose

empty sgRNA cloning vector with MS2-humanA3a D131A

Depositor

Fei-Long Meng

Article

Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

Insert

human Apobec3a D131A mutant (APOBEC3A Human)

Use

CRISPR

Expression

Mammalian

Mutation

D131A mutant in hA3a

Promoter

hU6,CBh

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMAL c2x Tral FL-15D

Plasmid

#113010

Purpose

For bacterial expression of MBP fusion of full-length Drosophila Tral with Phos-mimic mutations

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

full length tral (tral Fly)

Expression

Bacterial

Mutation

15 PNG phosphorylation sites mutated to D

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pET-30a(+)-Syk-tSH2-FX

Plasmid

#111272

Purpose

express murine Syk tandem SH2 domains (Ser 8 to Gln 264), with interdomain A (Phe 119 to His 162) substituted by a flexible 20-amino-acid linker (GGS)3GS(GGS)3, in E.coli strain Rosetta 2 (DE3)

Depositor

Carol Post

Article

Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

Insert

murine Syk tandem SH2 domains (Ser 8 to Gln 264), with interdomain A (Phe 119 to His 162) substituted by a flexible 20aa linker (Syk Mouse)

Expression

Bacterial

Mutation

interdomain A (Phe 119 to His 162) substituted by…

Promoter

T7 promoter

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-AuroraB L154A/H250Y

Plasmid

#108489

Purpose

expression of vsv-AuroraB Analog sensitive (L154A/H250Y)

Depositor

Susanne Lens

Article

Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

Insert

AuroraB (AURKB Human)

Tags

VSV

Expression

Mammalian

Mutation

Analog sensitive L154A/H250Y

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pJC20cdc8.A18T

Plasmid

#99360

Purpose

Bacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Ala-18-Thr.

Depositor

Dan Mulvihill

Article

Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

Insert

cdc8 (cdc8 Fission Yeast)

Expression

Bacterial

Mutation

Alanine 18 to Threonine

Promoter

T7

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pJC20cdc8.E31K

Plasmid

#99361

Purpose

Bacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Glu-31-Lys.

Depositor

Dan Mulvihill

Article

Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

Insert

cdc8 (cdc8 Fission Yeast)

Expression

Bacterial

Mutation

Glutamic acid 31 to Lysine

Promoter

T7

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pMCh-1538

Plasmid

#82431

Purpose

Plasmid for expression of NHA-tagged human-Nematostella GW182 11W11A chimera in mammalian cells

Depositor

Marina Chekulaeva

Article

Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

Insert

hs-nvGW182 11W11A chimera

Tags

NHA

Expression

Mammalian

Mutation

aa 1-1169 of siRNA resistant hsTNRC6A (Q8NDV7-2) …

Promoter

CMV

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-G596C

Plasmid

#131712

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

G596C

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-G469S

Plasmid

#131716

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

G469S

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-V600R

Plasmid

#131721

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

V600R

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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PBAN-tetOn-NKX3.1

Plasmid

#238472

Purpose

Doxycycline inducible expression of the NKX3.1 transcription factor for mural cell differentiation.

Depositor

Juan Melero-Martin

Article

Lee et al Nat Commun. 2024 Sep 30;15(1):8392. doi:

Insert

NKX3.1 (NKX3-1 Human)

Expression

Mammalian

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1a_Flag_TP53_WT

Plasmid

#183112

Purpose

Expresses flag-tagged p53 in mammalian cells

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

TP53 (TP53 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

See Depositor Comments Below

Promoter

EF1a

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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PCR4-Shh::lacZ-H11

Plasmid

#139098

Purpose

enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)

Depositor

Len Pennacchio

Article

Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

Shh

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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