We narrowed to 11,712 results for: NSI

1. pSBbi-FoxO1_1R_10A_3D

   Plasmid

   #106278

   Purpose

   Fluorescent fusion protein for FoxO1

   Depositor

   Laura Heiser

   Article

   Gross et al Cell Syst. 2019 Dec 18;9(6):580-588.e4.

   Inserts

   Foxo1a (Foxo1 Mouse)
   NLS-mCherry-NLS-P2A-Puromycin
   

   Tags

   Clover fluorescent protein

   Expression

   Mammalian

   Mutation

   Deleted amino acids 401-636, S209A, H212R, S215A,…

   Promoter

   EF1a/RPBSA

   Available Since

   July 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSET-iSeroSnFR

   Plasmid

   #128483

   Purpose

   Fluorescent reporter for serotonin (bacterial expression)

   Depositor

   Lin Tian

   Article

   Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

   Insert

   iSeroSnFR
   

   Tags

   His, T7, and cpsfGFP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223-ZNF423

   Plasmid

   #88083

   Purpose

   Donor Vector containing ZNF423 transcription factor, part of the Human TFome Collection

   Depositor

   Marc Vidal

   Article

   Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

   Insert

   ZNF423 (ZNF423 Human)
   

   Use

   Gateway donor vector

   Mutation

   Last nucleotide of stop codon removed to allow fo…

   Available Since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR207 SARS-CoV-2 NSP3_nostop

   Plasmid

   #149306

   Purpose

   Gateway-compatible Entry vector, with insert of NSP3 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP3 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pnCS_SEPT7_SEPT9_i3-TEV-Strep

   Plasmid

   #174501

   Purpose

   bacterial co-expression of human SEPT7 and of human SEPT9_i3

   Depositor

   Manos Mavrakis

   Article

   Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

   Inserts

   SEPTIN7 (SEPTIN7 Human)
   SEPTIN9 (SEPTIN9 Human)
   

   Tags

   TEV-Strep

   Expression

   Bacterial

   Available Since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMAL-Abl-PRM 4R

   Plasmid

   #112087

   Purpose

   Bacterial expression plasmid containing His and MBP tags for 4 PRM motif repeats of Human Abl.

   Depositor

   Michael Rosen

   Article

   Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

   Insert

   Abl-PRM-4R (ABL1 Human)
   

   Tags

   His-6 and MBP

   Expression

   Bacterial

   Mutation

   construct contains only the PRM motif of Abl1

   Promoter

   Lac

   Available Since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Syn-miRFP

   Plasmid

   #108416

   Purpose

   Monomeric near-infrared fluorescent protein miRFP in mammalian-expressing AAV production plasmid

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   miRFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-nEF-Con/Fon-NpHR3.3-EYFP

   Plasmid

   #137152

   Purpose

   Intersectional viral expression of NpHR3.3-EYFP in cells expressing both Cre AND Flp

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Fon-NpHR3.3-EYFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   W179F

   Promoter

   nEF

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOmnibac_ZZ_linker_K963_GFP_SNAP

   Plasmid

   #159727

   Purpose

   Expresses kinesin-1 (amino acids 1-963) in Sf9 cells with a C-terminal GFP and SNAP tag

   Depositor

   Ahmet Yildiz

   Article

   Ferro et al Science. 2022 Jan 21;375(6578):326-331.

   Insert

   KIF5B (full length, 1-963 amino acids), K963 (KIF5B Human)
   

   Tags

   GFP and SNAP tags and ZZ affinity tag

   Expression

   Insect

   Available Since

   Jan. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. TERT - R865C

    Plasmid

    #213926

    Purpose

    Expresses Mutant TERT R865C

    Depositor

    Coleman Lindsley

    Article

    Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

    Insert

    Mutant TERT R865C (TERT Human)
    

    Use

    Lentiviral

    Tags

    6x His Tag

    Expression

    Mammalian

    Mutation

    Arginine 865 changed to Cysteine

    Promoter

    Tet-responsive promoter PTight, consisting of sev…

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CaMKII-Archon1-EGFP

    Plasmid

    #108417

    Purpose

    AAV production plasmid encoding for Archon1 fluorescent voltage reporter

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Archon1-EGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CaMKII

    Available Since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSPL3-ABCC8-9-10

    Plasmid

    #135918

    Purpose

    Encodes ABCC8 exons 9 and 10 wild type for the analysis of splicing variants

    Depositor

    Diana Valverde Pérez

    Article

    Lago-Docampo et al Sci Rep. 2020 Sep 15;10(1):15135. doi: 1

    Insert

    ATP-binding Cassette subfamily C member 8 (ABCC8 Human)
    

    Use

    Exon trapping

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-ADAR2-2a-Fezf2 sesRNA-2a-tTA2-WPRE

    Plasmid

    #239027

    Purpose

    Expression of ADAR2, sesRNA in mammalian cells with hSyn promoter, with tTA2 as efRNA and ADAR2 overexpression.

    Depositor

    Josh Huang

    Article

    Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

    Insert

    sesRNA for mouse Fezf2 (Fezf2 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNOC_dfnCas12a-KRAB_crRNA NC

    Plasmid

    #176262

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and spacer sequence is replaced by the type IIS restriction site for endonucleas

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    Kruppel associated box domain and luciferase

    Mutation

    D917A and E1006A mutations to inactivate the endo…

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-nEF-Con/Foff 2.0-ChRmine-oScarlet

    Plasmid

    #137161

    Purpose

    Intersectional viral expression of ChRmine-p2a-oScarlet in cells expressing Cre AND NOT Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Foff 2.0-ChRmine-p2a-oScarlet
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Mutation

    oScarlet E95D

    Promoter

    nEF

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFP5299 ss-FIREmate-HA- KDEL-IVS-IRES- ManII-SBP- mApple-FIREtag

    Plasmid

    #216695

    Purpose

    Synchronize anterograde and retrograde trafficking of ManII between ER and Golgi (RUCH system)

    Depositor

    Franck Perez

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Inserts

    FIREmate-KDEL
    ManII-SBP-mApple-FIREtag (Man2a1 Mouse)
    

    Tags

    FIREtag, Streptavidin Binding Protein (SBP), and …

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-puro/RAF1-S259A

    Plasmid

    #131727

    Purpose

    Mammalian Expression of RAF1

    Depositor

    Jacques De Grève

    Article

    Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

    Insert

    RAF1 (RAF1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    S259A

    Promoter

    CMV

    Available Since

    May 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNOC_dCas9-KRAB_sgRNA Td2

    Plasmid

    #176264

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged to the KRAB domain and Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    dead spCas9
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    Kruppel associated box domain and luciferase

    Mutation

    H840A and D10A mutations on spCas9 to inactivate …

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR-TBP(53Q)-IDR-mCherry-Cry2-NLS

    Plasmid

    #145270

    Purpose

    Mammalian Expression of Nuclear Opto TBP(53Q) IDR with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    TBP(53Q)-IDR (TBP Human)
    

    Expression

    Mammalian

    Mutation

    Q-repeat expanded

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. dCas9-NUP98a_IDR-EGFP

    Plasmid

    #232766

    Purpose

    Expresses dCas9-NUP98a_IDR-EGFP

    Depositor

    Isaac Hilton

    Article

    Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

    Insert

    dCas9-NUP98a_IDR-EGFP (NUP98 Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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