We narrowed to 10,356 results for: plasmids 101

1. HNF1B_pLX307

   Plasmid

   #98342

   Purpose

   Lentiviral expression of HNF1B

   Depositor

   Sefi Rosenbluh, William Hahn

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   HNF1B (HNF1B Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOTTC1124 - pAAV MeCP2 SpCas9(D10A)

   Plasmid

   #112719

   Purpose

   An AAV vector that expresses SpCas9 nickase under a neuronal cell promoter

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   SpCas9
   

   Use

   AAV

   Tags

   Expression

   Mutation

   D10A

   Promoter

   Mecp2

   Available Since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAG-DIO-PPO-Venus

   Plasmid

   #139504

   Purpose

   AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.

   Depositor

   Michael Bruchas

   Article

   Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

   Has Service

   AAV5

   Insert

   parapinopsin
   

   Use

   AAV

   Tags

   Venus tag following 3x Ala linker

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOTTC468 - pAAV EF1a DIO hChR2(H134R)-iRFP

   Plasmid

   #47633

   Purpose

   An AAV vector that expresses channel rhodopsin 2 (H134R) (fused to iRFP) in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   hChR2 (H134R)
   

   Use

   AAV and Cre/Lox

   Tags

   iRFP

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   EF1a

   Available Since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. Tet-O-FUW-GLI1-P2A-mCherry

   Plasmid

   #203858

   Purpose

   Tet-inducible lentiviral plasmid expressing GLI1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

   Depositor

   Andrea Califano

   Article

   Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

   Insert

   GLI1 (GLI1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Tet-O-FUW-MAFB-P2A-mCherry

   Plasmid

   #203866

   Purpose

   Tet-inducible lentiviral plasmid expressing MAFB-P2A-mCherry, with ORF specific barcode close to 3'LTR site

   Depositor

   Andrea Califano

   Article

   Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

   Insert

   MAFB (MAFB Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLZRS-ER-Rac1-Q61L-shortlinker

   Plasmid

   #128584

   Purpose

   For mammalian cell expression of Q61L mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHT

   Depositor

   Julian Downward

   Article

   Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

   Insert

   Rac1 (Rac1 Mouse)
   

   Use

   Retroviral

   Tags

   Modified hormone binding domain of ER alpha

   Expression

   Mammalian

   Mutation

   Changed Glutamine61 to Leucine in the RAC1 moiety.

   Promoter

   Available Since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLZRS-ER-Rac1-P29S-shortlinker

   Plasmid

   #128583

   Purpose

   For mammalian cell expression of P29S mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHT

   Depositor

   Julian Downward

   Article

   Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

   Insert

   Rac1 (Rac1 Mouse)
   

   Use

   Retroviral

   Tags

   Modified hormone binding domain of ER alpha

   Expression

   Mammalian

   Mutation

   Changed Proline29 to Serine in the RAC1 moiety.

   Promoter

   Available Since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXs-Sox2-17

   Plasmid

   #193353

   Purpose

   Retroviral vector expressing mouse Sox2-17 chimeric transcription factor (super-Sox)

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   Sox2-17 (Sox2 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Sox2-17 is engineered highly cooperative chimeric…

   Promoter

   5'LTR

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Tet-O-FUW-KLF4-P2A-mCherry

    Plasmid

    #203855

    Purpose

    Tet-inducible lentiviral plasmid expressing KLF4-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    KLF4 (KLF4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Tet-O-FUW-ID2-P2A-mCherry

    Plasmid

    #203865

    Purpose

    Tet-inducible lentiviral plasmid expressing ID2-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    ID2 (ID2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSBbi-moxGFP-P2A-T2A-cMyc-Puro

    Plasmid

    #176892

    Purpose

    Sleeping Beauty transposon-based vector for mammalian polycistronic expression of moxGFP, c-Myc and an additional gene of interest

    Depositor

    Jorge Luis Arias-Arias

    Article

    Acon et al iScience. 2021 Nov 8;24(12):103407. doi:

    Insert

    moxGFP-P2A-T2A-c-Myc
    

    Use

    Transposon

    Tags

    Myc and moxGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. MIGR1-Cdc42-1

    Plasmid

    #179517

    Purpose

    Retroviral expression of Cdc42 splice variant 1

    Depositor

    Bo Porse

    Article

    Rehn et al Cell Rep. 2022 May 10;39(6):110793. doi:

    Insert

    Cdc42 splice variant 1 cDNA (CDC42 Human)
    

    Use

    Retroviral

    Tags

    IRES-EGFP

    Expression

    Mutation

    Promoter

    LTR

    Available Since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOTTC1440 - pAAV CMV-IE Nuc-iRFP-2A-Flpo

    Plasmid

    #112684

    Purpose

    An AAV vector that expresses a nuclear-localized iRFP713 reporter and optimized Flp recombinase

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    iRFP713
    

    Use

    AAV

    Tags

    2A-Flpo and Nuclear localization signal

    Expression

    Mutation

    Promoter

    CMV-IE

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Tet-O-FUW-NR3C1-P2A-mCherry

    Plasmid

    #203854

    Purpose

    Tet-inducible lentiviral plasmid expressing NR3C1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    NR3C1 (NR3C1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL3-Basic-ACSL3-sfGFP(C) HDR template

    Plasmid

    #129413

    Purpose

    HDR tempalte for tagging of endogenous human ACSL3 C-terminus with sfGFP

    Depositor

    Elina Ikonen

    Article

    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

    Insert

    ACSL3 HDR template (ACSL3 Human)
    

    Use

    CRISPR and TALEN; Endogenous tagging hdr template

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. MIGR1-Cdc42-2

    Plasmid

    #179518

    Purpose

    Retroviral expression of Cdc42 splice variant 2

    Depositor

    Bo Porse

    Article

    Rehn et al Cell Rep. 2022 May 10;39(6):110793. doi:

    Insert

    Cdc42 splice variant 2 cDNA (CDC42 Human)
    

    Use

    Retroviral

    Tags

    IRES-EGFP

    Expression

    Mutation

    Promoter

    LTR

    Available Since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti-LucAL

    Plasmid

    #174049

    Purpose

    Expresses mALG8 (ITYTWTRL) and mLAMA4 (VGFNFRTL) antigens as a fusion to luciferase and Cre recombinase

    Depositor

    Tyler Jacks

    Article

    Burger et al Cell. 2021 Sep 16;184(19):4996-5014.e26.

    Insert

    LucAL
    

    Use

    Cre/Lox, Lentiviral, and Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    ALG8 alanine 506 to threonine; LAMA4 glycine 1254…

    Promoter

    Human Ubiquitin C

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA3 ATP5O(5’UTR)-SL-FF

    Plasmid

    #85491

    Purpose

    Firefly luciferase under the control of ATP5O 5'UTR followed by a stem-loop

    Depositor

    Ivan Topisirovic

    Article

    Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

    Insert

    5'UTR of ATP5O followed by stem loop (ATP5PO Human)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ERK1C202

    Plasmid

    #164647

    Purpose

    Bacterial expression plasmid for His6-ERK1C202

    Depositor

    Ben Davis

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    ERK1C202 (MAPK1 Human)
    

    Use

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    K71R/C82S/C144S/C178S/C183S/T202C/C271S

    Promoter

    T7

    Available Since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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