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We narrowed to 21,502 results for: SPR

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Showing: 1681 - 1720 of 21502 results

  14. lentiCRISPRv2_PROX1_1+4

    Plasmid
    #121177
    Purpose
    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
    Depositor
    Timo Otonkoski
    Insert
    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    Use
    CRISPR and Lentiviral
    Tags
    P2A-puro
    Expression
    Mammalian
    Available Since
    March 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    121177-plasmid-map-sequence-id-237896

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  15. LentiCRISPR_Hs_MBD3_ex2

    Plasmid
    #62623
    Purpose
    Encodes gRNA which targets human MBD3
    Depositor
    Paul Wade
    Insert
    MBD3 gRNA (MBD3 Human)
    Use
    CRISPR and Lentiviral
    Promoter
    EFS
    Available Since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  16. LentiCRISPR_Hs_MBD3_ex3

    Plasmid
    #62624
    Purpose
    Encodes gRNA which targets human MBD3
    Depositor
    Paul Wade
    Insert
    MBD3 gRNA (MBD3 Human)
    Use
    CRISPR and Lentiviral
    Promoter
    EFS
    Available Since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  17. pLentiRNACRISPR_002 - hU6-DR_BsmBI-EFS-PguCas13b-NES-2A-Puro-WPRE

    Plasmid
    #138144
    Purpose
    Expresses PguCas13b in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    PguCas13b
    Use
    Lentiviral
    Tags
    NES
    Available Since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138144-plasmid-map-sequence-id-272529

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  22. pCDNA3-Flag-SPRTN-Y117C

    Plasmid
    #110216
    Purpose
    Expression in mammalian cells of of full-length SPRTN protein carrying Y117C mutation, a catalytically-impaired mutant, and Flag-tag at N-terminus.
    Depositor
    Ivana Marinovic-Terzic, Kristijan Ramadan
    Article
    Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi
    Insert
    SPRTN (SPRTN Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Y117C, P296L (see depositor comments below)
    Available Since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    110216-plasmid-map-sequence-id-210941

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  23. R777-E328 Hs.SPRY1-nostop

    Plasmid
    #70612
    Purpose
    Gateway ORF clone of human SPRY1 [NM_001258038.1] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SPRY1 (SPRY1 Human)
    Use
    Gateway Cloning
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70612-plasmid-map-sequence-id-126061

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  24. R777-E334 Hs.SPRY4-nostop

    Plasmid
    #70617
    Purpose
    Gateway ORF clone of human SPRY4 [NM_001127496.1] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SPRY4 (SPRY4 Human)
    Use
    Gateway Cloning
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70617-plasmid-map-sequence-id-133434

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  25. lentiCRISPR - Amplicon, JAK2 sgRNA 2

    Plasmid
    #70660
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic JAK2 amplicon-targeting sgRNA element from U6 promoter. Lentiviral backbone
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against JAK2 amplicon found in AML-derived HEL cell line (JAK2 )
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  29. lentiCRISPR-V2-PGK-Zeo

    Plasmid
    #240074
    Purpose
    Version of lentiCRISPR-V2 with EF1 promoter replaced with mouse PGK, zeocin resistance
    Depositor
    Raj Chari
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    March 18, 2026
    Availability
    Academic Institutions and Nonprofits only
    240074-plasmid-map-sequence-id-477091

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  30. lentiCRISPR-V2-PGK-GFP

    Plasmid
    #240075
    Purpose
    Version of lentiCRISPR-V2 with EF1 promoter replaced with mouse PGK, GFP
    Depositor
    Raj Chari
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    March 18, 2026
    Availability
    Academic Institutions and Nonprofits only
    240075-plasmid-map-sequence-id-481728

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  34. pHelper-CRISPRi-sgRNA

    Plasmid
    #221135
    Purpose
    Helper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.
    Depositor
    Craig Roy
    Article
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    sgRNA-scaffold with dCas9 handle
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    J23119(SpeI)
    Available Since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221135-plasmid-map-sequence-id-436077

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Showing: 1681 - 1720 of 21502 results