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Plasmid#195198Purposeall-optical silencing methodDepositorInserteHcKCR1 3.0-oScarlet-Kv2.1
UseAAVTagsExpressionMutationPromoterCaMKIIaAvailable sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
Syn-ATP
Plasmid#51819PurposeOptical reporter of presynaptic ATPDepositorInsertChimeric Synaptophysin-mCherry-Luciferase
UseTagsExpressionMammalianMutationWithin WT luciferase gene, changed Threonine 214 …PromoterCMVAvailable sinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mEGFP-CaMKIIa (T286A)
Plasmid#127390PurposeFluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286A)DepositorInsertcalcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
UseTagsmEGFPExpressionMammalianMutationchanged Threonine 286 to AlaninePromoterpCAGAvailable sinceJan. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDule-Tet2.0
Plasmid#85496PurposePlasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertTetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
UseTagsNoneExpressionBacterialMutationY32G L65Q F108S Q109D D158S L162NPromoterlpp (constitutive)Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro_CMV_NES-Caprola_on-mEGFP
Plasmid#194694PurposeCMV driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression through lentivirus transductionDepositorInsertCaprola_on-mEGFP
UseLentiviralTagsmEGFPExpressionMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PA-mCherry1-Mito-7
Plasmid#57189PurposeLocalization: Mitochondria, Excitation: 400 / 504, Emission: 515 / 517DepositorInsertMito (COX8A Human)
UseTagsPA-mCherry1ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Caprola_on-mEGFP
Plasmid#194686PurposeCMV driven expression of the consitutively active calcium recorder positive control Caprola_on in mammalian cell lines fused to mEGFPDepositorInsertCaprola_on-mEGFP
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
D28 Cortactin GFP
Plasmid#26723DepositorInsertCortactin (Cttn Mouse)
UseTagsEGFPExpressionMammalianMutationDeletion removed 28 amino acids (aa# 334-361) enc…PromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV2_hSyn_NES-Caprola_04-mEGFP_WRPE-SV40
Plasmid#194688PurposehSyn1 driven expression of the calcium recorder Caprola_04 fused to mEGFP for neuronal expression through AAV transductionDepositorInsertCaprola_04-mEGFP
UseAAVTagsmEGFPExpressionMutationPromoterhSyn1Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mEGFP-CaMKIIa (T305D/T306D)
Plasmid#127611PurposeFluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T305D/T306D)DepositorInsertcalcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
UseTagsmEGFPExpressionMammalianMutationchanged Threonine 305/306 to AlaninePromoterpCAGAvailable sinceJan. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
PA-mCherry1-LaminB1-10
Plasmid#57186PurposeLocalization: Nuclear Envelope, Excitation: 400 / 504, Emission: 515 / 517DepositorInsertLaminB1 (LMNB1 Human)
UseTagsPA-mCherry1ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
DF-UAS-sspa-GCaMP6f
Plasmid#67569PurposeZebrafish expression of a short photoactivatable fluorescent calcium reporterDepositorInsertsspa-GCaMP6f
UseZebrafish transgenesis (see :a spinal opsin contr…TagsExpressionMutationK65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…PromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3-B-pCR
Plasmid#46369DepositorInsertcalretinin promoter (CALB2 Human)
UseLuciferaseTagsluciferaseExpressionMutationpromoterPromoterAvailable sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
DF-UAS-spa-GCaMP6f
Plasmid#67568PurposeZebrafish expression of a photoactivatable fluorescent calcium reporterDepositorInsertspa-GCaMP6f
UseZebrafish transgenesis (see :a spinal opsin contr…TagsHIS-tagExpressionMutationK65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…PromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV2_hSyn_NES-Caprola_05-mEGFP_WRPE-SV40
Plasmid#194689PurposehSyn1 driven expression of the calcium recorder Caprola_05 fused to mEGFP for neuronal expression through AAV transductionDepositorInsertCaprola_05-mEGFP
UseAAVTagsmEGFPExpressionMutationPromoterhSyn1Available sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTol2_elavl3(HuC)_NES-Caprola_on-mEGFP
Plasmid#194697PurposeHuC driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression in zebrafishDepositorInsertCaprola_on-mEGFP
UseZebrafish expressionTagsmEGFPExpressionMutationPromoterelavl3(HuC)Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTol2_elavl3(HuC)_NES-Caprola_off-mEGFP
Plasmid#194698PurposeHuC driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression in zebrafishDepositorInsertCaprola_off-mEGFP
UseZebrafish expressionTagsmEGFPExpressionMutationPromoterelavl3(HuC)Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV/CaMKIIa::iLMO2
Plasmid#129481PurposeAAV2 transfer plasmid for iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemical, under control of the glutamatergic-selective CaMKIIa promoterDepositorInsertiLMO2
UseAAVTagsExpressionMammalianMutationPromoterCaMKIIaAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-cFos-XRI-V5
Plasmid#178058PurposeExpression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with V5 tag, under c-fos promoter.DepositorInsertXRI-V5
UseAAVTagsV5-MBPExpressionMammalianMutationPromoterMouse c-fos promoterAvailable sinceOct. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-jGCaMP8f-p10
Plasmid#162388PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8f
UseTags6xHisExpressionInsectMutationQ315LPromoterhsp70Available sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only