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psd44-Lactadherin46

Plasmid

#46830

Purpose

expression of human lactadherin in mammalian cells

Depositor

Agnese Mariotti

Insert

MFGE8 (MFGE8 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK promoter

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pENTR_mCherry

Plasmid

#139554

Purpose

mCherry ORF with stop codon between attL1-attL2 on Gateway entry vector

Depositor

Minoru Ko

Insert

mCherry

Expression

Mammalian

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-T7-KPNA5

Plasmid

#26681

Depositor

Bryce Paschal

Article

Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

Insert

Karyopherin Alpha 5 (KPNA5 Human)

Tags

T7 (MASMTGGQQMG)

Expression

Mammalian

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only

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mouse Oct4-GFP GOF18 transgenic reporter

Plasmid

#60527

Purpose

BAC recombineering generated construct, used as a reporter for Oct4 expression (containes both DE and PE elements)

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

GFPpA

Expression

Mammalian

Promoter

Oct4

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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2C-3XtbGFP-PEST

Plasmid

#69072

Purpose

Expresses 3XturboGFP-NLS-PEST under MERVL_LTR

Depositor

Maria-Elena Torres-Padilla

Article

Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

Insert

turboGFP

Expression

Mammalian

Available Since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.SFFV.GFP

Plasmid

#57827

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
SFFV
P2A-eGFP

Use

CRISPR and Lentiviral

Tags

FLAG

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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TRX1 flag C35S

Plasmid

#21285

Depositor

Wang Min

Article

Liu et al Circ Res. 2002 Jun 28. 90(12):1259-66.

Insert

TRX1 (TXN Human)

Tags

Flag

Expression

Mammalian

Mutation

C35S

Available Since

July 30, 2009

Availability

Academic Institutions and Nonprofits only
pRN114

Plasmid

#127221

Purpose

BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, Wus, AtSTM

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-nEFCas9

Plasmid

#87115

Purpose

SpCas9 expression AAV backbone plasmid under control of nEF promoter

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

nEF-Cas9

Use

AAV and CRISPR

Tags

HA-NLS

Expression

Mammalian

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-2A-TurboGFP-PEST

Plasmid

#67180

Purpose

CRISPaint universal donor for gene expression quantification using destabilized TurboGFP

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

T2A-TurboGFP-PEST

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pESF140_HisSpy_DDB1∆B

Plasmid

#124213

Purpose

Insect cell expression vector for His6, Spy tagged hsDDB1∆B

Depositor

Eric Fischer

Article

Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

Insert

DNA damage-binding protein 1 (DDB1 Human)

Tags

His6-Spy

Expression

Insect

Mutation

internal deletion of BPB domain to aid crystalliz…

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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PGK plasmid

Plasmid

#169744

Purpose

Unregulated "open-loop" PGK promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

eGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

PGK promoter

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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eGFP STAT1 S727E

Plasmid

#12305

Depositor

Alan Perantoni

Article

Timofeeva et al Oncogene. 2006 Dec 7;25(58):7555-64. doi

Insert

STAT1 S727E (STAT1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

S727E

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

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pMyNT-kan

Plasmid

#84693

Purpose

Protein expression in mycobacteria

Depositor

Matthias Wilmanns

Article

Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

Type

Empty backbone

Tags

His6

Expression

Bacterial

Promoter

Acetamidase

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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HA-14-3-3 eta

Plasmid

#116887

Purpose

Expresses HA-tagged human 14-3-3 eta in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Article

Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

Insert

14-3-3 eta (YWHAH Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCW57.1-FOXM1c

Plasmid

#68810

Purpose

Doxycycline inducible lentiviral vector for FOXM1c expression in mammalian cells

Depositor

Adam Karpf

Article

Barger et al Oncotarget. 2015 Jul 16.

Insert

FOXM1c (FOXM1 Human)

Use

Lentiviral; Doxycycline inducible

Tags

FLAG

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-hTERT-HA

Plasmid

#1772

Depositor

Bob Weinberg

Article

Counter et al Proc Natl Acad Sci U S A 1998 Dec 8;95(2

Insert

hTERT (TERT Human)

Use

Retroviral

Tags

2XHA

Expression

Mammalian

Available Since

June 3, 2005

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-ArchT-WPREpA

Plasmid

#84472

Purpose

pAAV-RAM-ArchT

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Insert

ArchT

Use

AAV

Tags

EGFP

Promoter

TRE

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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tdTomato-anti-AlfaTag nanobody

Plasmid

#171819

Purpose

Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

Depositor

Ikuo Wada

Article

Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

Insert

tdTomato-anti-AlfaTag nanobody

Tags

6xHis and SEP tag (6xD)

Expression

Bacterial

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pEYFP-C1-Drp1

Plasmid

#45160

Depositor

Richard Youle

Article

Frank et al Dev Cell. 2001 Oct;1(4):515-25.

Insert

Drp1 (DNM1L Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

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