We narrowed to 16,161 results for: nans

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pOP22-5'UTR-BBa_B0034

Plasmid

#216626

Purpose

NanoLuc reporter plasmid to optimize chloroplast cell-free systems with RBS BBa_B0034 in 5'UTR

Depositor

Henrike Niederholtmeyer

Article

Bohm et al ACS Synth Biol. 2024 Aug 16;13(8):2412-2

Insert

T7p-5'B0034-NanoLuc-3'TMV

Use

Synthetic Biology

Promoter

T7

Available Since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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xlox dNGFR-TERT

Plasmid

#69805

Purpose

MLV retroviral vector expressing the hTERT reverse transcriptase immortalizing gene and a tNGFR marker for tracking

Depositor

David Ott

Article

Andersen et al AIDS Res Hum Retroviruses. 2007 Mar;23(3

Insert

hTERT reverse transcriptase (TERT Human)

Use

Retroviral

Tags

none

Promoter

MLV promoter

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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Rabbit GFP Cav 2.2 pMT2

Plasmid

#58737

Purpose

expression of GFP tagged rabbit Cav2.2 in mammalian cells

Depositor

Annette Dolphin

Article

Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

Insert

Cav2.2 (CACNA1B Rabbit)

Tags

GFP

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-FGF21

Plasmid

#182407

Purpose

Bacterial expression of Vesicle Nucleating peptide-fgf21 fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-fgf21 (FGF21 Human)

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pBSFI-myr-akt-T308A/S473A

Plasmid

#49191

Purpose

cDNA of Akt1 with myristyolation signal and point mutation from T to A at position 308 and S to A at position 473 in cloning vector

Depositor

Peter Vogt

Article

Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

Insert

Akt1 (Akt1 Mouse)

Use

Adaptor plasmid

Tags

HA tag and myristolation signal

Mutation

T308A; S473A

Promoter

T7

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A24

Plasmid

#135507

Purpose

Mammalian expression of myc-tagged HLA-A*024:02

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*24:02 (HLA-A Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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CYFIP1.mCherry

Plasmid

#122051

Purpose

Expresses human CYFIP1 in mammalian cells.

Depositor

Josef Kittler

Article

Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

Insert

CYFIP1 (CYFIP1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-sRGECO

Plasmid

#137125

Purpose

Viral expression of sRGECO

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

sRGECO

Use

AAV

Mutation

E217D

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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plenti-UbcP-HA-CRBN-pGK-HYG

Plasmid

#107378

Purpose

Lentiviral expression of HA-CRBN

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

cereblon (CRBN Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

UbcP

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-TYMS_sgRNA1

Plasmid

#201628

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

TYMS (TYMS Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-BCR-ABL-p190-FLAG

Plasmid

#205620

Purpose

Expression of BCR-ABL oncogene in mammalian cells

Depositor

Pavel Krejčí

Article

Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

Insert

BCR-ABL oncogene, p190 isoform b3a2

Tags

FLAG

Expression

Mammalian

Mutation

T117M- Please see depositor comments

Promoter

CMV

Available Since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-375-Decoy.hPGK.GFP.WPRE

Plasmid

#46617

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-375 Decoy (MIR375, Mir375 Human, Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pAAV-CaMKIIa-DIO eHcKCR1 3.0-oScarlett-KV2.1

Plasmid

#195198

Purpose

all-optical silencing method

Depositor

Karl Deisseroth

Article

Fan et al Cell. 2023 Feb 2;186(3):543-559.e19. doi

Insert

eHcKCR1 3.0-oScarlet-Kv2.1

Use

AAV

Promoter

CaMKIIa

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-mCherry-IRES-Dre

Plasmid

#55633

Purpose

Expresses Dre in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Inserts

mCherry
Dre

Use

AAV

Expression

Mammalian

Promoter

Ef1a and Ef1a/IRES

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pGreenFire_blastocidin

Plasmid

#174103

Purpose

pGreenFire MPRA plasmid for cloning

Depositor

Paul Khavari

Article

Guo et al Nat Genet. 2023 Nov;55(11):1876-1891. do

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-cGAS C396/7A-HA

Plasmid

#130917

Purpose

Expresses human cGAS-HA with C396/397A point mutations; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

cGAS C396/7A

Tags

HA

Expression

Mammalian

Mutation

mutated residues 396 and 397 to alanines

Promoter

CMV

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pSW003-PpsbA-mNeonGreen

Plasmid

#205019

Purpose

Broad host-range bacterial expression vector with constitutive PsbA promoter; mNeonGreen (codon optimized for P. fluorescens)

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

mNeonGreen

Expression

Bacterial

Mutation

mNeonGreen is codon optimized for expression in P…

Promoter

PpsbA

Available Since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-FAS-hChR2(H134R)-mCherry-WPRE-pA

Plasmid

#37090

Purpose

Expresses humanized ChR2(H134R)-mCherry in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic excitation

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

hChr2(H134R)-mCherry

Use

AAV and Cre/Lox; Cre-off

Tags

mCherry

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pAmCyan1-C1-ADCY6

Plasmid

#113905

Purpose

N-terminal Cyan Fluorescent tag in Adenylate Cyclase 6

Depositor

Anjaparavanda Naren

Article

Thomas et al J Biol Chem. 2018 Jun 14. pii: RA118.003

Insert

Adenylate Cyclase 6 (ADCY6 Human)

Tags

Cyan

Expression

Bacterial and Mammalian

Promoter

pCMV

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-M13-TevC-IRES-SP6-Cre

Plasmid

#171620

Purpose

M13-TevC with Cre

Depositor

Hyungbae Kwon

Article

Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

Insert

M13-TevC-IRES-SP6-Cre

Use

AAV and Synthetic Biology

Tags

6x His

Expression

Mammalian

Promoter

hSyn

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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