We narrowed to 18,590 results for: IRE

1. pSPORT1[NheI/glGFP/bglobin3'-UTR/Ins2]

   Plasmid

   #74101

   Purpose

   vector bearing an NheI restriction site for the two-step cloning method in Wang and Szaro, Dev Biol, 2015

   Depositor

   Ben Szaro

   Article

   Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

   Inserts

   Green Lantern Green Fluorescent Protein
   Rabbit beta 1-globin gene 3'UTR
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS-KS-attB2-SA(1)-T2A-LexA::GADfluw-Hsp70

   Plasmid

   #78306

   Purpose

   Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 1

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-nlsLexA::GADfluw-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. BSD-P2A-TagRFP-hSmad1-donor

   Plasmid

   #203662

   Purpose

   Donor plasmid for CRISPR knock-in

   Depositor

   Ali Hemmati Brivanlou

   Article

   Yoney et al Elife. 2018 Oct 12;7:e38279. doi: 10.755

   Insert

   smad1 (SMAD1 Human)
   

   Use

   Tags

   RFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXs_FLAG-MFT

   Plasmid

   #136371

   Purpose

   Expression in mammalian cells

   Depositor

   David Sabatini

   Article

   Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

   Insert

   MFT (SLC25A32 Human)
   

   Use

   Retroviral

   Tags

   3XFlag

   Expression

   Mammalian

   Mutation

   codon-optimized

   Promoter

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGWB21_TaNAM-A1

   Plasmid

   #124572

   Purpose

   Expresses full-length TaNAM-A1 with an N-terminal 10x Myc tag under the 35s promoter.

   Depositor

   Cristobal Uauy

   Article

   Harrington et al BMC Plant Biol. 2019 Sep 18;19(1):407. d

   Insert

   TaNAM-A1
   

   Use

   Tags

   10xMyc

   Expression

   Plant

   Mutation

   Promoter

   35s

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWL502-crRNA

   Plasmid

   #174383

   Purpose

   The plasmids carrying a mini-CRISPR array were used to express crRNAs; article demonstrates use in Haloferax mediterranei

   Depositor

   Jing Han

   Article

   Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

   Insert

   a crRNA targeting the template strand
   

   Use

   CRISPR; Archaeal expression

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PhaR promoter

   Available since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti CRISPR sgSHMT2_1

   Plasmid

   #106308

   Purpose

   Express Cas9 and sgRNA targeting SHMT2

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   sgRNA targeting SHMT2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCETN1-IRFP670

   Plasmid

   #99879

   Purpose

   Expression of CETN1-iRFP670 in mammalian cells

   Depositor

   Marc Tramier

   Article

   Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

   Insert

   CETN1 (CETN1 Human)
   

   Use

   Tags

   iRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-Y-GECO1

   Plasmid

   #55766

   Purpose

   Encoded a yellow fluorescent calcium ion indicator with inverted fluorescence response for expression in mammalian cells

   Depositor

   Robert Campbell

   Article

   Zhao et al Integr Biol (Camb). 2014 May 19.

   Insert

   Y-GECO1.0
   

   Use

   Tags

   6x His tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRL K-ras 3'UTR mutant

    Plasmid

    #14805

    Depositor

    Tyler Jacks

    Article

    Kumar et al Nat Genet. 2007 Apr 1. ():.

    Insert

    K-ras 3'UTR (Kras Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    let-7 miRNA binding site mutations

    Promoter

    Available since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pET21-pelB-LaG-27

    Plasmid

    #172752

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-27
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR- flag-Lag16-Intb2

    Plasmid

    #205203

    Purpose

    This vector encodes of the synCAM (Intb2) and GFP nanobody LAG16

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM Intb2 and Lag16 anti-GFP nanobody
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. GAL4-mNFAT1(2-144)

    Plasmid

    #11108

    Purpose

    Expresses mouse NFAT1 (amino acids 2-144) fused to GAL4 DNA binding domain in mammalian cells

    Depositor

    Anjana Rao

    Article

    Garcia-Rodriguez et al Eur J Immunol. 2000 Aug . 30(8):2432-6.

    Insert

    NFAT1 (Nfatc2 Mouse)
    

    Use

    Tags

    GAL4-DBD

    Expression

    Mammalian

    Mutation

    Contains NFAT1 amino acids 2-144; L78P and P140Q …

    Promoter

    Available since

    April 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOPS0385

    Plasmid

    #133235

    Purpose

    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    constructed with NoP (pOGG113), gusA (pOGG083) and T-pharma (pOGG003)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOPS0386

    Plasmid

    #133236

    Purpose

    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    constructed with NoP (pOGG113), celB (pOGG050) and T-pharma (pOGG003)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    April 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOPS0383

    Plasmid

    #133233

    Purpose

    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    constructed with NoP (pOGG113), sfGFP (pOGG037) and T-pharma (pOGG003)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    April 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRRG304-Ndk

    Plasmid

    #99072

    Purpose

    dsRNA and riboprobe synthesis for Schmidtea mediterranea Ndk (nou darake)

    Depositor

    Phillip Newmark

    Article

    Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

    Insert

    Ndk (nou darake) in situ probe
    

    Use

    T/a cloning vector for dsrna generation and the g…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. NHA-Ago1

    Plasmid

    #21535

    Depositor

    Edward Chan

    Article

    Lian et al RNA. 2009 May . 15(5):804-13.

    Insert

    Ago1 (AGO1 Human)
    

    Use

    Tags

    NHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pLsCas13aGG

    Plasmid

    #131012

    Purpose

    Backbone plasmid for generating CRISPR arrays for LsCas13a. Contains a GFP-dropout cassette and a direct repeat.

    Depositor

    Chase Beisel

    Article

    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

    Inserts

    direct repeat of LsCas13a
    GFP expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCEP4-myc-H2-Ld

    Plasmid

    #135519

    Purpose

    Mammalian expression of myc-tagged H2-Ld

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    H2-Ld
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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